The present disclosure provides a three-dimensional display device, a driving method and manufacturing method therefor. The three-dimensional display device comprises: a display panel having a plurality of pixels sequentially arranged in row direction and column direction; a light splitting assembly located on the display side of the display panel and having a plurality of light splitting structures, the plurality of light splitting structures being arranged parallel to each other and sequentially arranged in row direction, wherein pixels covered by at least one light splitting structure are divided into at least one pixel repeating unit, and the at least one pixel repeating unit is sequentially arranged along an extension direction of the light splitting structure; light emitted by pixels at the same position in all of the pixel repeating units forms a viewpoint through respective corresponding light splitting structures; the width of each light splitting structure in row direction is D; each pixel repeating unit comprises at least two rows of pixels, and each row of pixels comprises N1 pixels, and aa; wherein L is the vertical distance from a human eye observation point to a light splitting structure, h is the vertical distance from the pixel to the light splitting structure, and Px is the size of the pixel in row direction.