发明申请
- 专利标题: Process for preparing purified active monomer of bone-derived factor
- 专利标题(中): 骨源性因子纯化活性单体的制备方法
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申请号: US10734583申请日: 2003-12-15
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公开(公告)号: US20050026247A1公开(公告)日: 2005-02-03
- 发明人: Hidetoshi Andou , Jun Honda , Shunjiro Sugimoto , Gertrud Hotten , Rolf Bechtold , Jens Pohl
- 申请人: Hidetoshi Andou , Jun Honda , Shunjiro Sugimoto , Gertrud Hotten , Rolf Bechtold , Jens Pohl
- 申请人地址: DE Heidelberg 69115
- 专利权人: Biopharma Gesellschaft zur Biotechnologischen Entwicklung von Pharmaka GmbH
- 当前专利权人: Biopharma Gesellschaft zur Biotechnologischen Entwicklung von Pharmaka GmbH
- 当前专利权人地址: DE Heidelberg 69115
- 优先权: JP8/355812 19961225; JP10/141379 19980522; EP99115613.4 19990806
- 主分类号: A61K38/00
- IPC分类号: A61K38/00 ; C07K14/475 ; C07K14/51 ; C07K14/575 ; C12N1/21 ; C07H21/04
摘要:
A process for preparing a purified refolded monomer or dimer of a bone-derived factor, which comprises subjecting an inclusion body of a bone-derived factor produced by genetic engineering to the following steps a) to c) in sequence: a) introducing a polynucleotide encoding a bone morphogenetic factor into a bacterium, expressing said bone morphogenetic factor in the form of an inclusion body, recovering said inclusion body and treating it with a denaturing agent to obtain a solubilized monomer, b) treating the solubilized monomer without purification directly with a refolding solution to obtain a refolded monomeric bone morphogenetic factor, c) subjecting the refolded monomeric bone morphogenetic factor to purification.