发明申请
- 专利标题: Glycoprotein and process for producing the same
- 专利标题(中): 糖蛋白及其制备方法
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申请号: US10480790申请日: 2002-06-14
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公开(公告)号: US20050064539A1公开(公告)日: 2005-03-24
- 发明人: Yasunori Chiba , Yoshifumi Jigami , Hitoshi Sakuraba , Kazuo Kobayashi , Makoto Takeuchi , Yoriko Takeuchi
- 申请人: Yasunori Chiba , Yoshifumi Jigami , Hitoshi Sakuraba , Kazuo Kobayashi , Makoto Takeuchi , Yoriko Takeuchi
- 优先权: JP2001-180907 20010614
- 国际申请: PCT/JP02/05965 WO 20020614
- 主分类号: C12N15/09
- IPC分类号: C12N15/09 ; A61K36/064 ; A61K38/00 ; A61K38/17 ; A61P9/00 ; A61P9/14 ; A61P13/12 ; A61P17/00 ; A61P19/00 ; A61P27/02 ; A61P43/00 ; C08B37/00 ; C12N9/10 ; C12N9/40 ; C12P21/00 ; C12P21/06 ; C12N1/18
摘要:
A process for producing a lysosomal enzyme having a mannose-6-phosphate-containing acidic sugar chain, wherein the process comprising: culturing in a medium yeast cells obtained by introducing a lysosomal enzyme gene into a sugar chain biosynthetic enzyme gene mutant strain of yeast, collecting a lysosomal enzyme having a phosphate-containing sugar chain from the culture, and then treating the enzyme with α-mannosidase; and pharmaceutical compositions for treatment of human lysosomal enzyme deficiencies produced by the process. The genetic engineering technique using the yeast according to the present invention allows large-amount and high-purity production of a glycoprotein having a phosphate-containing acidic sugar chain which can serve as a labeling marker for transporting into lysosomes in cells of mammals such as human. The glycoprotein having a phosphate-containing acidic sugar chain according to the invention may be utilized as a drug effective in treatment of human lysosomal enzyme deficiencies, etc.
公开/授权文献
- US07579166B2 Glycoprotein and process for producing the same 公开/授权日:2009-08-25