发明申请
- 专利标题: Zinc finger protein derivatives and methods therefor
- 专利标题(中): 锌指蛋白衍生物及其方法
-
申请号: US10941069申请日: 2004-09-14
-
公开(公告)号: US20050084885A1公开(公告)日: 2005-04-21
- 发明人: Carlos Barbas , Joel Gottesfeld , Peter Wright
- 申请人: Carlos Barbas , Joel Gottesfeld , Peter Wright
- 专利权人: THE SCRIPPS RESEARCH INSTITUTE
- 当前专利权人: THE SCRIPPS RESEARCH INSTITUTE
- 主分类号: A61K38/00
- IPC分类号: A61K38/00 ; A61K48/00 ; C07K14/46 ; C07K14/47 ; C12N15/62 ; C12Q1/68 ; G01N33/569 ; G01N33/68 ; C12N9/22
摘要:
Zinc finger proteins of the Cys2His2 type represent a class of malleable DNA binding proteins which may be selected to bind diverse sequences. Typically, zinc finger proteins containing three zinc finger domains, like the murine transcription factor Zif268 and the human transcription factor Spl, bind nine contiguous base pairs (bp). To create a class of proteins which would be generally applicable to target unique sites within complex genomes, the present invention provides a polypeptide linker that fuses two three-finger proteins. Two six-fingered proteins were created and demonstrated to bind 18 contiguous bp of DNA in a sequence specific fashion. Expression of these proteins as fusions to activation or repression domains allows transcription to be specifically up or down modulated within cells. Polydactyl zinc finger proteins are broadly applicable as genome-specific transcriptional switches in gene therapy strategies and the development of novel transgenic plants and animals. Such proteins are useful for inhibiting, activating or enhancing gene expression from a zinc finger-nucleotide binding motif containing promoter or other transcriptional control element, as well as a structural gene or RNA sequence.