An information storage medium which contains preload information, a reproducing apparatus and a reproducing method. The information storage medium includes AV data and a markup language document displaying the AV data which is decoded and reproduced and including preload information that allows the reproducing apparatus to read a file to be preloaded and to store it into a memory. The information storage medium contains the markup language document to prevent a video picture from being interrupted when the AV data recorded in the DVD is reproduced and displayed through the markup language document. The reproducing apparatus and reproducing method reproduce information from the information storage medium.