The present invention discloses a handler of a USB memory device, comprising a tray horizontally moving by a tray loader/unloader, a loading/unloading picking means transferring a case of a plurality of USB memory devices, a shuttle means horizontally moving by a shuttle transfer in a state where the upper surface of a plurality of mounted USB memory devices is supported by a plunger, and a test board mounted by a test socket comprising a plurality of USB ports enabling electrical connection in such a way that the projected USB connector is inserted into the shuttle means horizontally moving by the shuttle transfer.