发明申请
US20050220767A1 Production of recombinant respiratory syncytial viruses expressing immune modulatory molecules
审中-公开
表达免疫调节分子的重组呼吸道合胞病毒的生产
- 专利标题: Production of recombinant respiratory syncytial viruses expressing immune modulatory molecules
- 专利标题(中): 表达免疫调节分子的重组呼吸道合胞病毒的生产
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申请号: US10917984申请日: 2004-08-11
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公开(公告)号: US20050220767A1公开(公告)日: 2005-10-06
- 发明人: Peter Collins , Alexander Bukreyev , Brian Murphy , Stephen Whitehead
- 申请人: Peter Collins , Alexander Bukreyev , Brian Murphy , Stephen Whitehead
- 主分类号: C12N15/09
- IPC分类号: C12N15/09 ; A61K9/12 ; A61K35/76 ; A61K39/00 ; A61K39/155 ; A61K48/00 ; A61P11/00 ; A61P11/06 ; A61P31/14 ; A61P37/04 ; C07K14/54 ; C12N7/00 ; C12N7/04 ; C12N15/86 ; A61K39/12
摘要:
Recombinant respiratory syncytial virus (RSV) are provided which express one or more immune modulatory molecules. The recombinant virus is modified by addition or substitution of a polynucleotide sequence encoding the immune modulatory molecule, which is preferably a cytokine. Introduction of the cytokine increase, decrease, or otherwise enhances aspects of viral biology and/or host immune responses to RSV to facilitate vaccine use of the virus. Cytokines for use within the invention include but are not limited to interleukin 2 (IL-2), interleukin 4 (IL-4), interleukin 5 (IL-5), interleukin 6 (IL-6), or interleukin 18 (IL-18), tumor necrosis factor (TNF) alpha, interferon gamma (IFN), and granulocyte-macrophage colony stimulating factor (GM-CSF). The polynucleotide or immune modulatory molecule is preferably added or substituted into the recombinant viral genome or antigenome, typically at an intergenic or other non-coding site, as a separate gene but may be otherwise expressed, for example as a fusion protein.
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