发明申请
- 专利标题： Preparation, characterization and quantification of active fractions that promote cell growth and recombinant-protein expression
- 专利标题（中）： 促进细胞生长和重组蛋白表达的活性级分的制备，表征和定量
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申请号： US10637017申请日： 2003-08-07
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公开(公告)号： US20050250177A1公开(公告)日： 2005-11-10
- 发明人: Faustinus Yeboah , Barbara Jardin , Yasuo Konishi , Amine Kamen , Tin Wong , Soo Lee
- 申请人： Faustinus Yeboah , Barbara Jardin , Yasuo Konishi , Amine Kamen , Tin Wong , Soo Lee
- 主分类号： C12N5/00
- IPC分类号： C12N5/00 ; C12N5/02 ; C12N5/06 ; C12P21/06
摘要：
A process for preparing an active animal cell-growth-enhancing fraction of a hydrolysate of plant tissue, animal tissue or microorganism ultrafiltrate material, especially a fraction of a yeastolate ultrafiltrate, comprising forming a precipitate from said fraction with a water-soluble solvent is disclosed. Such solvents as alkanols, alkyl sulfoxides, ketones or alkyl nitriles, especially lower (C1-C5) alkanols, particularly ethanol, can be used. The invention also relates to an active animal cell-growth-enhancing fraction of a hydrolysate of plant tissue, animal tissue or microorganism ultrafiltrate material, especially a fraction of a yeastolate ultrafiltrate, substantially free of aromatic and methyl group bearing compounds which has improved cell-growth-enhancing properties.
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