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    Preparation, characterization and quantification of active fractions that promote cell growth and recombinant-protein expression
    1.
    发明申请
    Preparation, characterization and quantification of active fractions that promote cell growth and recombinant-protein expression 审中-公开
    促进细胞生长和重组蛋白表达的活性级分的制备，表征和定量

    公开(公告)号：US20050250177A1

    公开(公告)日：2005-11-10

    申请号：US10637017

    申请日：2003-08-07

    申请人： Faustinus Yeboah ,  Barbara Jardin ,  Yasuo Konishi ,  Amine Kamen ,  Tin Wong ,  Soo Lee

    发明人： Faustinus Yeboah ,  Barbara Jardin ,  Yasuo Konishi ,  Amine Kamen ,  Tin Wong ,  Soo Lee

    IPC分类号： C12N5/00 ,  C12N5/02 ,  C12N5/06 ,  C12P21/06

    CPC分类号： C12N5/0018 ,  C12N2500/70 ,  C12N2500/74 ,  C12N2500/92

    摘要： A process for preparing an active animal cell-growth-enhancing fraction of a hydrolysate of plant tissue, animal tissue or microorganism ultrafiltrate material, especially a fraction of a yeastolate ultrafiltrate, comprising forming a precipitate from said fraction with a water-soluble solvent is disclosed. Such solvents as alkanols, alkyl sulfoxides, ketones or alkyl nitriles, especially lower (C1-C5) alkanols, particularly ethanol, can be used. The invention also relates to an active animal cell-growth-enhancing fraction of a hydrolysate of plant tissue, animal tissue or microorganism ultrafiltrate material, especially a fraction of a yeastolate ultrafiltrate, substantially free of aromatic and methyl group bearing compounds which has improved cell-growth-enhancing properties.

    摘要翻译： 公开了一种用于制备植物组织，动物组织或微生物超滤液物质的水解产物的活性动物细胞生长增强部分的方法，特别是酵母醇酯超级滤液的一部分，包括用水溶性溶剂从所述级分形成沉淀物 。 可以使用诸如链烷醇，烷基亚砜，酮或烷基腈，特别是较低（C 1 -C 5 -C 5）烷醇，特别是乙醇的溶剂。 本发明还涉及植物组织，动物组织或微生物超滤液物质的水解产物的活性动物细胞生长增强级分，特别是基本上不含芳香族和甲基基团化合物的酵母醇酯超级滤液的一部分， 生长增强性能。