- 专利标题： Microwell array chip for detecting antigen-specific lymphocytes, method of detecting and method of manufacturing antigen-specific lymphocytes, and method of cloning antigen-specific lymphocyte antigen receptor genes
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申请号： US10534800申请日： 2003-09-30
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公开(公告)号： US20060134704A1公开(公告)日： 2006-06-22
- 发明人: Atsushi Muraguchi , Hiroyuki Kishi , Eiichi Tamiya , Masayasu Suzuki
- 申请人： Atsushi Muraguchi , Hiroyuki Kishi , Eiichi Tamiya , Masayasu Suzuki
- 优先权： JP2002-331031 20021114; JP2002-346728 20021129
- 国际申请： PCT/JP03/12500 WO 20030930
- 主分类号： G01N33/567
- IPC分类号： G01N33/567 ; C12M1/34 ; G01N33/53
摘要：
A microwell array chip that has multiple microwells and is employed to contain a single lymphocyte specimen in each microwell and detect antigen-specific lymphocytes in single units; wherein the microwell array chip is of a shape and of dimensions where only one lymphocyte is contained in each microwell. A method of detecting antigen-specific lymphocytes comprising the steps of adding antigen to each microwell in the above microwell array chip, stimulating the lymphocyte specimen, and detecting lymphocyte specimens reacting with the antigen.
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