发明申请
US20060141570A1 Intein-mediated protein purification using in vivo expression of an aggregator protein
审中-公开
使用体内表达聚集蛋白的蛋白质介导的蛋白纯化
- 专利标题： Intein-mediated protein purification using in vivo expression of an aggregator protein
- 专利标题（中）： 使用体内表达聚集蛋白的蛋白质介导的蛋白纯化
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申请号： US11274254申请日： 2005-11-16
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公开(公告)号： US20060141570A1公开(公告)日： 2006-06-29
- 发明人: David Wood , Mahmoud Banki , Tillman Gerngross
- 申请人： David Wood , Mahmoud Banki , Tillman Gerngross
- 主分类号： C12P21/06
- IPC分类号： C12P21/06 ; C07H21/04 ; C12P7/62 ; C12N9/18 ; C12N1/21 ; C12N15/74
摘要：
Purification of recombinant proteins is performed by expressing in a host cell a fusion protein comprising: (a) a product protein domain, (b) an intein, and (c) at least one aggregator protein domain, wherein the aggregator protein domain comprises a protein that is capable of specific association with granules of polyhydroxyalkanoate (PHA).
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