- 专利标题: Materials and methods for detection of nucleic acids
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申请号: US11486841申请日: 2006-07-14
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公开(公告)号: US20060252091A1公开(公告)日: 2006-11-09
- 发明人: David Marshall , James Prudent , Christopher Sherrill , Gideon Shapiro , Jennifer Grenier , Craig Richmond , Simona Jurczyk , Jerod Ptacin
- 申请人: David Marshall , James Prudent , Christopher Sherrill , Gideon Shapiro , Jennifer Grenier , Craig Richmond , Simona Jurczyk , Jerod Ptacin
- 专利权人: EraGen Biosciences, Inc.
- 当前专利权人: EraGen Biosciences, Inc.
- 主分类号: C12Q1/68
- IPC分类号: C12Q1/68
摘要:
Assays using non-natural bases are described. In one embodiment, the method involves contacting a sample suspected of containing the target nucleic acid with a polymerase and first and second primers; amplifying the target nucleic acid, if present in the sample, by PCR using the first and second primers to generate an amplification product having a double-stranded region and a single-stranded region that comprises the non-natural base; contacting the sample with a reporter comprising a label and a non-natural base that is complementary to the non-natural base of the single-stranded region; annealing at least a portion of the reporter to the single-stranded region of the amplification product; and correlating a signal of the label with the presence of the target nucleic acid in the sample. The invention also provides corresponding kits for use in detecting target nucleic acids in a sample.
公开/授权文献
- US07517651B2 Materials and methods for detection of nucleic acids 公开/授权日:2009-04-14