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    Materials and methods for the detection of severe acute respiratory syndrome virus (SARS)
    2.
    发明申请
    Materials and methods for the detection of severe acute respiratory syndrome virus (SARS) 审中-公开
    检测严重急性呼吸综合征病毒（SARS）的材料和方法

    公开(公告)号：US20070059686A1

    公开(公告)日：2007-03-15

    申请号：US11494820

    申请日：2006-07-28

    申请人： Michael Moser ,  James Prudent

    发明人： Michael Moser ,  James Prudent

    IPC分类号： C12Q1/70 ,  C12Q1/68

    CPC分类号： C12Q1/701 ,  C12Q2525/101

    摘要： Disclosed are methods and kits for identifying a virus in a sample, which may include a coronavirus capable of causing Severe Acute Respiratory Syndrome (“SARS”) or SARS-like symptoms. The virus may be a SARS-Associated Coronavirus (“SARS-CoV”). Typically, the methods may include reacting a mixture that includes, in addition to nucleic acid isolated from the sample, at least one oligonucleotide capable of specifically hybridizing to SARS-CoV nucleic acid where the oligonucleotide includes at least one non-natural base. In addition, the mixture may include control nucleic acid.

    摘要翻译： 公开了用于鉴定样品中的病毒的方法和试剂盒，其可以包括能够引起严重急性呼吸综合征（SARS）或SARS样症状的冠状病毒。 病毒可能是SARS相关冠状病毒（“SARS-CoV”）。 通常，所述方法可以包括使除了从样品分离的核酸之外的至少一种能够与SARS-CoV核​​酸特异性杂交的寡核苷酸的混合物反应，其中寡核苷酸包括至少一个非天然碱基。 此外，混合物可以包括对照核酸。

    Nucleic acid cleavage assays
    6.
    发明申请
    Nucleic acid cleavage assays 审中-公开
    核酸切割测定

    公开(公告)号：US20070292856A1

    公开(公告)日：2007-12-20

    申请号：US11489665

    申请日：2006-07-19

    申请人： Victor Lyamichev ,  Michael Kaiser ,  Natalie Lyamichev ,  Jeff Hall ,  James Prudent ,  Mary Brow

    发明人： Victor Lyamichev ,  Michael Kaiser ,  Natalie Lyamichev ,  Jeff Hall ,  James Prudent ,  Mary Brow

    IPC分类号： C12Q1/68

    CPC分类号： C12N9/22 ,  C12Q1/6813 ,  C12Q2521/301

    摘要： The present invention provides novel cleavage agents and polymerases for the cleavage and modification of nucleic acid. The cleavage agents and polymerases find use, for example, for the detection and characterization of nucleic acid sequences and variations in nucleic acid sequences. In some embodiments, the 5′ nuclease activity of a variety of enzymes is used to cleave a target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof.

    摘要翻译： 本发明提供了用于切割和修饰核酸的新型切割剂和聚合酶。 裂解剂和聚合酶可用于例如核酸序列的检测和表征以及核酸序列的变化。 在一些实施方案中，使用各种酶的5'核酸酶活性来切割靶依赖性切割结构，从而指示特异性核酸序列的存在或其特定变体。

    USE OF NON-STANDARD BASES AND PROXIMITY EFFECTS FOR GENE ASSEMBLY AND CONVERSION OF NON-STANDARD BASES TO STANDARD BASES DURING DNA SYNTHESIS
    7.
    发明申请
    USE OF NON-STANDARD BASES AND PROXIMITY EFFECTS FOR GENE ASSEMBLY AND CONVERSION OF NON-STANDARD BASES TO STANDARD BASES DURING DNA SYNTHESIS 审中-公开
    非标准基因的使用和基因组合的遗传效应和DNA合成期间标准基因的非标准转换

    公开(公告)号：US20070264694A1

    公开(公告)日：2007-11-15

    申请号：US11733011

    申请日：2007-04-09

    申请人： James Prudent

    发明人： James Prudent

    IPC分类号： C12P19/34

    CPC分类号： C12P19/34 ,  C12N15/10 ,  C12N15/1096

    摘要： The present methods relate to generating nucleic acid molecules using non-natural nucleotides. In some methods, the nucleic acid molecules may be generated by hybridizing a plurality of oligonucleotides comprising one or more non-natural nucleotides and using a polymerase and/or a coupling agent to link the hybridized oligonucleotides. The methods also relate to the use of proximity effects to generate nucleic acid molecules using non-natural nucleotides. Furthermore, the methods relate to the use of at least one non-natural base in a DNA template in order to generate a replicate of the DNA template in which the non-natural base has been replaced with a natural base.

    摘要翻译： 本发明的方法涉及使用非天然核苷酸产生核酸分子。 在一些方法中，核酸分子可以通过杂交包含一个或多个非天然核苷酸的多个寡核苷酸并使用聚合酶和/或偶联剂来连接杂交的寡核苷酸来产生。 所述方法还涉及使用邻近效应以使用非天然核苷酸产生核酸分子。 此外，所述方法涉及在DNA模板中使用至少一种非天然碱基以产生其中非天然碱被天然碱替代的DNA模板的重复。