- 专利标题: Methods for the electrochemical detection of multiple target compounds
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申请号: US11511004申请日: 2006-08-28
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公开(公告)号: US20060292624A1公开(公告)日: 2006-12-28
- 发明人: H. Thorp , Ivana Yang , David Stewart , John Groelke , Veronika Szalai
- 申请人: H. Thorp , Ivana Yang , David Stewart , John Groelke , Veronika Szalai
- 主分类号: C12Q1/68
- IPC分类号: C12Q1/68 ; C12M1/34
摘要:
A method of detecting two different target molecules through a single electrode is carried out by (a) providing a conductive oxidation-reduction reaction detection electrode; (b) contacting a sample suspected of containing a first and second target molecule to the electrode under conditions in which the first and second target molecules are deposited on the electrode, wherein the first target molecule comprises a first label and the second target molecule comprises a second label; (c) contacting to the electrode a first transition metal complex that oxidizes the first preselected label in a first oxidation-reduction reaction and a second transition metal complex that oxidizes the first and second labels in a second oxidation-reduction reaction, with the first and second oxidation-reduction reactions producing different detectable signals; (d) detecting the presence of the first target molecule by detecting the first oxidation-reduction reaction; and(e) detecting the presence of the second target molecule by detecting the second oxidation-reduction reaction. Devices for carrying out the method are also described.