公开(公告)号：US20060292624A1

公开(公告)日：2006-12-28

申请号：US11511004

申请日：2006-08-28

申请人： H. Thorp ,  Ivana Yang ,  David Stewart ,  John Groelke ,  Veronika Szalai

发明人： H. Thorp ,  Ivana Yang ,  David Stewart ,  John Groelke ,  Veronika Szalai

IPC分类号： C12Q1/68 ,  C12M1/34

CPC分类号： C12Q1/005 ,  C12Q1/004 ,  C12Q1/6825 ,  C12Q2565/101 ,  C12Q2563/113

摘要： A method of detecting two different target molecules through a single electrode is carried out by (a) providing a conductive oxidation-reduction reaction detection electrode; (b) contacting a sample suspected of containing a first and second target molecule to the electrode under conditions in which the first and second target molecules are deposited on the electrode, wherein the first target molecule comprises a first label and the second target molecule comprises a second label; (c) contacting to the electrode a first transition metal complex that oxidizes the first preselected label in a first oxidation-reduction reaction and a second transition metal complex that oxidizes the first and second labels in a second oxidation-reduction reaction, with the first and second oxidation-reduction reactions producing different detectable signals; (d) detecting the presence of the first target molecule by detecting the first oxidation-reduction reaction; and(e) detecting the presence of the second target molecule by detecting the second oxidation-reduction reaction. Devices for carrying out the method are also described.