发明申请
US20070105173A1 Modified pyrroloquinoline quinone (pqq) dependent glucose dehydrogenase excellent in substrate specificity
失效
修饰的吡咯喹啉醌（pqq）依赖性葡萄糖脱氢酶底物特异性优异
- 专利标题： Modified pyrroloquinoline quinone (pqq) dependent glucose dehydrogenase excellent in substrate specificity
- 专利标题（中）： 修饰的吡咯喹啉醌（pqq）依赖性葡萄糖脱氢酶底物特异性优异
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申请号： US10570904申请日： 2004-08-31
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公开(公告)号： US20070105173A1公开(公告)日： 2007-05-10
- 发明人: Seiji Takeshima , Tadanobu Matsumura , Takahide Kishimoto , Masanori Oka , Noriaki Hirayama
- 申请人： Seiji Takeshima , Tadanobu Matsumura , Takahide Kishimoto , Masanori Oka , Noriaki Hirayama
- 申请人地址： JP Osaka-shi 530-8230
- 专利权人： Toyo Boseki Kabushiki Kaisha
- 当前专利权人： Toyo Boseki Kabushiki Kaisha
- 当前专利权人地址： JP Osaka-shi 530-8230
- 优先权： JP2003-315797 20030908; JP2003-315799 20030908; JP2004-060283 20040304; JP2004-060282 20040304; JP2004-150905 20040521
- 国际申请： PCT/JP04/12508 WO 20040831
- 主分类号： C12Q1/54
- IPC分类号： C12Q1/54 ; C07H21/04 ; C12P21/06 ; C12N9/02 ; C12M1/34
摘要：
PQQGDH having an improved substrate specificity or having an improved specific activity in an assay system using ferricyanide ion as a mediator is provided. Modified PQQGDH having the enhanced substrate specificity by introducing an amino acid mutation in a particular region of PQQGDH, and a method of enhancing the specific activity compared with a wild type in the assay system using the ferricyanide ion as the mediator by deleting, substituting, or adding one or more amino acids in an amino acid sequence of the wild type pyrroloquinoline quinone dependent glucose dehydrogenase.
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