发明申请
US20070154950A1 Method of quickly detecting and/or assaying antigen by fluorescence correlation spectrometry
失效
通过荧光相关光谱法快速检测和/或测定抗原的方法
- 专利标题: Method of quickly detecting and/or assaying antigen by fluorescence correlation spectrometry
- 专利标题(中): 通过荧光相关光谱法快速检测和/或测定抗原的方法
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申请号: US11633345申请日: 2006-12-04
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公开(公告)号: US20070154950A1公开(公告)日: 2007-07-05
- 发明人: Masataka Kinjo , Motohiro Horiuchi , Fumihiko Fujii , Hiroshi Sakata , Mamoru Tamura , Masayoshi Ueno , Takayuki Yanagiya
- 申请人: Masataka Kinjo , Motohiro Horiuchi , Fumihiko Fujii , Hiroshi Sakata , Mamoru Tamura , Masayoshi Ueno , Takayuki Yanagiya
- 优先权: JP2004-166440 20040603
- 主分类号: G01N33/53
- IPC分类号: G01N33/53
摘要:
The present invention provides a method of quickly and accurately detecting and/or assaying an antigen using fluorescence correlation spectroscopy (FCS), which involves a fluorescence-labeled antibody fragment and a non-fluorescence-labeled intact antibody that form a complex with the antigen. There is a significant difference in diffusion rate between the fluorescence-labeled antibody fragment not bonded to the antigen and the complex formed by the the fluorescence-labeled antibody fragment, the antigen, and the non-fluorescence-labeled intact antibody, and this diffusion rate can be determined using FCS. The antigen can be an antigenic protein, such as an abnormal prion or a harmful protein contained in a food material. According to this method, antigens over a wide scope can be assayed regardless of the shape or molecular weight.