公开(公告)号：US20070154950A1

公开(公告)日：2007-07-05

申请号：US11633345

申请日：2006-12-04

申请人： Masataka Kinjo ,  Motohiro Horiuchi ,  Fumihiko Fujii ,  Hiroshi Sakata ,  Mamoru Tamura ,  Masayoshi Ueno ,  Takayuki Yanagiya

发明人： Masataka Kinjo ,  Motohiro Horiuchi ,  Fumihiko Fujii ,  Hiroshi Sakata ,  Mamoru Tamura ,  Masayoshi Ueno ,  Takayuki Yanagiya

IPC分类号： G01N33/53

CPC分类号： G01N33/6896 ,  G01N33/536 ,  G01N33/582 ,  G01N33/6857 ,  G01N2800/2828

摘要： The present invention provides a method of quickly and accurately detecting and/or assaying an antigen using fluorescence correlation spectroscopy (FCS), which involves a fluorescence-labeled antibody fragment and a non-fluorescence-labeled intact antibody that form a complex with the antigen. There is a significant difference in diffusion rate between the fluorescence-labeled antibody fragment not bonded to the antigen and the complex formed by the the fluorescence-labeled antibody fragment, the antigen, and the non-fluorescence-labeled intact antibody, and this diffusion rate can be determined using FCS. The antigen can be an antigenic protein, such as an abnormal prion or a harmful protein contained in a food material. According to this method, antigens over a wide scope can be assayed regardless of the shape or molecular weight.

摘要翻译： 本发明提供使用荧光相关光谱法（FCS）快速准确地检测和/或测定抗原的方法，其包括与抗原形成复合物的荧光标记的抗体片段和非荧光标记的完整抗体。 未结合抗原的荧光标记的抗体片段与由荧光标记的抗体片段，抗原和非荧光标记的完整抗体形成的复合物之间的扩散速率存在显着差异，并且该扩散速率 可以使用FCS确定。 抗原可以是抗原蛋白，例如食物中含有的异常朊病毒或有害蛋白质。 根据该方法，无论形状或分子量如何，可以测定广泛范围内的抗原。

公开(公告)号：US20090253217A1

公开(公告)日：2009-10-08

申请号：US12066284

申请日：2006-09-08

申请人： Kenta Saito ,  Hiroshi Sakata ,  Fumihiko Fujii ,  Masataka Kinjo ,  Mamoru Tamura

发明人： Kenta Saito ,  Hiroshi Sakata ,  Fumihiko Fujii ,  Masataka Kinjo ,  Mamoru Tamura

IPC分类号： G01N33/566

CPC分类号： G01N33/536 ,  G01N33/582 ,  G01N33/6896 ,  G01N2021/6439 ,  G01N2800/2828

摘要： The present invention is to provide a method of quickly detecting an antigen at an arbitrary concentration in an antigen sample, without a multi-stage examination of the concentration ratio between a detection reagent and an antigen to be detected, particularly when the concentration of the antigen in the sample is unknown, in the method of detecting an antigen using fluorescence correlation spectroscopy (FCS) or fluorescence cross-correlation spectroscopy (FCCS). By preparing (1) a series to which only a detection reagent is added and (2) a series to which an antigen and the detection reagent are added to achieve a maximum trimer concentration, and by performing a fluorescence spectroscopic analysis, the presence or absence of the antigen in the detection sample is quickly detected by the presence or absence of a trimer detection signal from a detector in the cases of (1) and (2), in a method of detecting an antigen by FCS or FCCS using as a detection reagent a fluorescent-labeled intact antibody or fluorescent-labeled antibody fragment targeted to an epitope of the antigen to be detected, and a non-fluorescent-labeled intact antibody or fluorescent-labeled intact antibody or fluorescent-labeled antibody fragment targeted to another epitope of the antigen.

摘要翻译： 本发明提供一种在抗原样品中以任意浓度快速检测抗原的方法，而不需要对检测试剂和待检测抗原之间的浓度比进行多阶段检查，特别是当抗原浓度 在样品中，在使用荧光相关光谱法（FCS）或荧光相关光谱法（FCCS）检测抗原的方法中是未知的。 通过制备（1）仅添加检测试剂的系列和（2）添加抗原和检测试剂以达到最大三聚体浓度的一系列序列，通过进行荧光光谱分析，存在或不存在 在（1）和（2）的情况下，通过检测器的三聚体检测信号的存在或不存在，通过FCS或FCCS检测抗原的方法来检测检测样品中的抗原， 试剂荧光标记的完整抗体或靶向要检测的抗原表位的荧光标记的抗体片段，以及靶向另一表位的非荧光标记的完整抗体或荧光标记的完整抗体或荧光标记的抗体片段 抗原。

公开(公告)号：US07476545B2

公开(公告)日：2009-01-13

申请号：US11633345

申请日：2006-12-04

申请人： Masataka Kinjo ,  Motohiro Horiuchi ,  Fumihiko Fujii ,  Hiroshi Sakata ,  Mamoru Tamura ,  Masayoshi Ueno ,  Takayuki Yanagiya

发明人： Masataka Kinjo ,  Motohiro Horiuchi ,  Fumihiko Fujii ,  Hiroshi Sakata ,  Mamoru Tamura ,  Masayoshi Ueno ,  Takayuki Yanagiya

IPC分类号： G01N21/76

CPC分类号： G01N33/6896 ,  G01N33/536 ,  G01N33/582 ,  G01N33/6857 ,  G01N2800/2828

摘要： The present invention provides a method of quickly and accurately detecting and/or assaying an antigen using fluorescence correlation spectroscopy (FCS), which involves a fluorescence-labeled antibody fragment and a non-fluorescence-labeled intact antibody that form a complex with the antigen. There is a significant difference in diffusion rate between the fluorescence-labeled antibody fragment not bonded to the antigen and the complex formed by the the fluorescence-labeled antibody fragment, the antigen, and the non-fluorescence-labeled intact antibody, and this diffusion rate can be determined using FCS. The antigen can be an antigenic protein, such as an abnormal prion or a harmful protein contained in a food material. According to this method, antigens over a wide scope can be assayed regardless of the shape or molecular weight.

摘要翻译： 本发明提供使用荧光相关光谱法（FCS）快速准确地检测和/或测定抗原的方法，其包括与抗原形成复合物的荧光标记的抗体片段和非荧光标记的完整抗体。 未结合抗原的荧光标记的抗体片段与由荧光标记的抗体片段，抗原和非荧光标记的完整抗体形成的复合物之间的扩散速率存在显着差异，并且该扩散速率 可以使用FCS确定。 抗原可以是抗原蛋白，例如食物中含有的异常朊病毒或有害蛋白质。 根据该方法，无论形状或分子量如何，可以测定广泛范围内的抗原。

公开(公告)号：US20080099728A1

公开(公告)日：2008-05-01

申请号：US11663545

申请日：2005-09-22

申请人： Takashi Jin ,  Masataka Kinjo ,  Mamoru Tamura ,  Fumihiko Fujii ,  Hiroshi Sakata

发明人： Takashi Jin ,  Masataka Kinjo ,  Mamoru Tamura ,  Fumihiko Fujii ,  Hiroshi Sakata

IPC分类号： C09K11/02

CPC分类号： A61K49/0067 ,  A61K49/0058 ,  B82Y5/00 ,  B82Y30/00 ,  C09K11/025 ,  C09K11/06 ,  C09K2211/1425

摘要： The present invention provides a water-soluble fluorescent material having a luminescent region at a visible range and having excellent luminescent efficiency. The present invention relates to a water-soluble fluorescent material having a semiconductor nanocrystal; a linear or cyclic phenol compound having hydrophilic group and hydrophobic group that coats at least a portion of the surface of the semiconductor nanocrystal; and a coordinating organic compound coating at least a portion of the surface of the semiconductor nanocrystal.

摘要翻译： 本发明提供一种水溶性荧光材料，其具有可见光范围的发光区域，发光效率优异。 本发明涉及具有半导体纳米晶体的水溶性荧光材料; 具有亲水基团和疏水基团的直链或环状酚化合物，其涂覆半导体纳米晶体的至少一部分表面; 以及在半导体纳米晶体的表面的至少一部分上的配位有机化合物涂层。