Invention Application
US20070172875A1 Compositions and Methods for Nucleic Acid Extraction from Biological Samples
审中-公开
从生物样品中提取核酸的组合物和方法
- Patent Title: Compositions and Methods for Nucleic Acid Extraction from Biological Samples
- Patent Title (中): 从生物样品中提取核酸的组合物和方法
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Application No.: US11688077Application Date: 2007-03-19
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Publication No.: US20070172875A1Publication Date: 2007-07-26
- Inventor: Scott Weber , Derek Douglas , Carol Kreader
- Applicant: Scott Weber , Derek Douglas , Carol Kreader
- Applicant Address: US MO St. Louis 63103
- Assignee: SIGMA-ALDRICH CO.
- Current Assignee: SIGMA-ALDRICH CO.
- Current Assignee Address: US MO St. Louis 63103
- Main IPC: C12Q1/68
- IPC: C12Q1/68 ; C12P19/34 ; C12N1/08
Abstract:
Methods and compositions for extracting nucleic acids from a biological sample are provided. The extraction compositions contain a protease enzyme such as proteinase K at alkaline pH with little or no surfactant present. Extraction can be efficiently performed in 60 minutes or less at room temperature for certain mammalian tissue samples and at elevated temperatures for certain plant tissues.
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