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公开(公告)号：US09671393B2
公开(公告)日：2017-06-06
申请号：US13821181
申请日：2011-09-02
Applicant: Vikas B. Palhan , Carol Kreader , Ernie Mueller
Inventor： Vikas B. Palhan , Carol Kreader , Ernie Mueller
CPC classification number: G01N33/5308 , C12N1/20 , G01N1/44 , G01N33/6803 , G01N33/6875
Abstract: The present invention provides isolated, non-viable bacterial cells comprising a plurality of nucleic acid crosslinks, methods for making said cells, and methods for using said cells to isolate DNA-protein complexes. In particular, the nucleic acids of the cells are crosslinked by contacting the cells with a furocoumarin compound and ultraviolet light.
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公开(公告)号：US20130172198A1
公开(公告)日：2013-07-04
申请号：US13821181
申请日：2011-09-02
Applicant: Vikas B. Palhan , Carol Kreader , Ernie Mueller
Inventor： Vikas B. Palhan , Carol Kreader , Ernie Mueller
CPC classification number: G01N33/5308 , C12N1/20 , G01N1/44 , G01N33/6803 , G01N33/6875
Abstract: The present invention provides isolated, non-viable bacterial cells comprising a plurality of nucleic acid crosslinks, methods for making said cells, and methods for using said cells to isolate DNA-protein complexes. In particular, the nucleic acids of the cells are crosslinked by contacting the cells with a furocoumarin compound and ultraviolet light.
Abstract translation: 本发明提供了分离的非活细菌细胞，其包含多个核酸交联，制备所述细胞的方法，以及使用所述细胞分离DNA-蛋白复合物的方法。 特别地，通过使细胞与呋喃香豆素化合物和紫外光接触来使细胞的核酸交联。
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3.发明申请Compositions and Methods for Nucleic Acid Extraction from Biological Samples 审中-公开从生物样品中提取核酸的组合物和方法公开(公告)号：US20070172875A1
公开(公告)日：2007-07-26
申请号：US11688077
申请日：2007-03-19
Applicant: Scott Weber , Derek Douglas , Carol Kreader
Inventor： Scott Weber , Derek Douglas , Carol Kreader
CPC classification number: C12N15/1003
Abstract: Methods and compositions for extracting nucleic acids from a biological sample are provided. The extraction compositions contain a protease enzyme such as proteinase K at alkaline pH with little or no surfactant present. Extraction can be efficiently performed in 60 minutes or less at room temperature for certain mammalian tissue samples and at elevated temperatures for certain plant tissues.
Abstract translation: 提供了从生物样品中提取核酸的方法和组合物。 提取组合物在碱性pH下含有蛋白酶K，例如蛋白酶K，几乎不存在或不存在表面活性剂。 对于某些哺乳动物组织样品，在某些植物组织的高温下，可以在室温下在60分钟以内有效地进行提取。
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公开(公告)号：US20080023395A1
公开(公告)日：2008-01-31
申请号：US11461235
申请日：2006-07-31
Applicant: Fuqiang Chen , Carol Kreader
Inventor： Fuqiang Chen , Carol Kreader
IPC: B01D39/00
CPC classification number: C12N15/101
Abstract: The present invention provides charged separation media, kits comprising the separation media, and methods of using the same to bind and optionally isolate biological molecules. The charged separation media and methods utilize a polyion non-covalently bound to the separation media to bind the desired biological molecule to the separation media.
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公开(公告)号：US20070166750A1
公开(公告)日：2007-07-19
申请号：US11687960
申请日：2007-03-19
Applicant: Scott Weber , Derek Douglas , Carol Kreader
Inventor： Scott Weber , Derek Douglas , Carol Kreader
CPC classification number: C12N15/1003
Abstract: Methods and compositions for extracting nucleic acids from a biological sample are provided. The extraction compositions contain a protease enzyme such as proteinase K at alkaline pH with little or no surfactant present. Extraction can be efficiently performed in 60 minutes or less at room temperature for certain mammalian tissue samples and at elevated temperatures for certain plant tissues.
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Patent Agency Ranking
公开(公告)号：US20150079657A1
公开(公告)日：2015-03-19
申请号：US14381381
申请日：2013-02-28
Applicant: Qingzhou Ji , Carol Kreader
Inventor： Qingzhou Ji , Carol Kreader
Abstract: The present disclosure provides compositions and methods for acetylating histones at targeted chromosomal locations in a cell. In particular, the disclosure provides a fusion protein comprising a DNA binding domain and at least one histone acetyltransferase (HAT) domain, such that the DNA binding domain targets the fusion protein to a targeted chromosomal location and the HAT domain acetylates histones at the targeted location.
Abstract translation: 本公开提供了在细胞中靶向染色体位置处乙酰化组蛋白的组合物和方法。 具体地，本公开提供了包含DNA结合结构域和至少一种组蛋白乙酰转移酶（HAT）结构域的融合蛋白，使得DNA结合结构域将融合蛋白靶向靶向染色体位置，并且HAT结构域在靶位置乙酰化组蛋白 。
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7.发明申请REAL TIME POLYMERASE CHAIN REACTION PROCESS USING A UNIVERSAL DETECTION SYSTEM 审中-公开使用通用检测系统的实时聚合酶链反应过程公开(公告)号：US20110136118A1
公开(公告)日：2011-06-09
申请号：US12996403
申请日：2009-06-16
Applicant: Carol Kreader , Brian Ward
Inventor： Carol Kreader , Brian Ward
IPC: C12Q1/68
CPC classification number: C12Q1/6823 , C12Q1/6851 , C12Q2561/113 , C12Q2561/109 , C12Q2537/149 , C12Q2521/301 , C12Q2565/1015 , C12Q2525/161 , C12Q2525/131
Abstract: Provided are methods and kits for detecting amplification of a target nucleic acid during a real time quantitative polymerase chain reaction process using a universal detection system. The detection system uses an unlabeled probe that detects the amplified target nucleic acid and interacts with a universal detection module.
Abstract translation: 提供了用于在使用通用检测系统的实时定量聚合酶链反应过程中检测靶核酸的扩增的方法和试剂盒。 检测系统使用检测扩增的靶核酸并与通用检测模块相互作用的未标记的探针。
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公开(公告)号：US20070166751A1
公开(公告)日：2007-07-19
申请号：US11688019
申请日：2007-03-19
Applicant: Scott Weber , Derek Douglas , Carol Kreader
Inventor： Scott Weber , Derek Douglas , Carol Kreader
CPC classification number: C12N15/1003
Abstract: Methods and compositions for extracting nucleic acids from a biological sample are provided. The extraction compositions contain a protease enzyme such as proteinase K at alkaline pH with little or no surfactant present. Extraction can be efficiently performed in 60 minutes or less at room temperature for certain mammalian tissue samples and at elevated temperatures for certain plant tissues.
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9.发明授权公开(公告)号：US07214484B2
公开(公告)日：2007-05-08
申请号：US10322103
申请日：2002-12-17
Applicant: Scott A. Weber , Derek K. Douglas , Carol Kreader
Inventor： Scott A. Weber , Derek K. Douglas , Carol Kreader
CPC classification number: C12N15/1003
Abstract: Methods and compositions for extracting nucleic acids from a biological sample are provided. The extraction compositions contain a protease enzyme such as proteinase K at alkaline pH with little or no surfactant present. Extraction can be efficiently performed in 60 minutes or less at room temperature for certain mammalian tissue samples and at elevated temperatures for certain plant tissues.
Abstract translation: 提供了从生物样品中提取核酸的方法和组合物。 提取组合物在碱性pH下含有蛋白酶K，例如蛋白酶K，几乎不存在或不存在表面活性剂。 对于某些哺乳动物组织样品，在某些植物组织的高温下，可以在室温下在60分钟以内有效地进行提取。
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公开(公告)号：US20080026374A1
公开(公告)日：2008-01-31
申请号：US11461263
申请日：2006-07-31
Applicant: Fuqiang Chen , Carol Kreader
Inventor： Fuqiang Chen , Carol Kreader
CPC classification number: C12Q1/6813
Abstract: The present invention provides charged separation media, kits comprising the separation media, and methods of using the same to bind and optionally isolate biological molecules. The charged separation media and methods utilize a polyion non-covalently bound to the separation media to bind the desired biological molecule to the separation media.
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