发明申请
US20070254332A1 Method for Readily Detecting Class C Beta-Lactamase-Producing Bacteria
审中-公开
易于检测C类β-内酰胺酶生产细菌的方法
- 专利标题: Method for Readily Detecting Class C Beta-Lactamase-Producing Bacteria
- 专利标题(中): 易于检测C类β-内酰胺酶生产细菌的方法
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申请号: US10582799申请日: 2004-12-14
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公开(公告)号: US20070254332A1公开(公告)日: 2007-11-01
- 发明人: Tetsuya Yagi , Jun-ichi Wachino , Yoshichika Arakawa
- 申请人: Tetsuya Yagi , Jun-ichi Wachino , Yoshichika Arakawa
- 申请人地址: JP CHUO-KU, TOKYO 103-0001
- 专利权人: JAPAN HEALTH SCIENCES FOUNDATION
- 当前专利权人: JAPAN HEALTH SCIENCES FOUNDATION
- 当前专利权人地址: JP CHUO-KU, TOKYO 103-0001
- 优先权: JP2003-416165 20031215
- 国际申请: PCT/JP04/18630 WO 20041214
- 主分类号: C12Q1/04
- IPC分类号: C12Q1/04
摘要:
A method for determining whether a test bacterium is a class C beta-lactamase-producing bacterium. Spots of a class C beta-lactamase inhibitor and a beta-lactam drug are applied at an interval on the surface of a solid medium that has been coated with the test bacterium, the solid medium is cultured, and following culturing, determining whether or not the inhibitory zone formed around the beta-lactam drug has extended toward the class C beta-lactamase inhibitor. A mixture of class C beta-lactamase inhibitor and beta-lactam drug and a beta-lactam drug in spots are applied at an interval on the surface of a solid medium that has been coated with the test bacterium, the solid medium is cultured, and following culturing, observing the difference between the inhibitory zone formed around the mixture and the inhibitory zone formed around the beta-lactam drug. A kit for determining class C beta-lactamase-producing bacteria, in which a disk containing a class C beta-lactamase inhibitor and a disk containing a beta-lactam drug are arranged on a striplike base. A kit for determining class C beta-lactamase-producing bacteria, in which a disk containing both a class C beta-lactamase inhibitor and a beta-lactam drug and a disk containing a beta-lactam drug are arranged on a striplike base. Provided are a method for readily detecting class C beta-lactamas-producing bacterium and kits performing the method.
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