An embodiment of the invention relates to a device and method for measuring small fluid volume and detecting an analyte in a sample. The device has a channel having a plurality of sensors in a wall of the channel, wherein the sensors are located along a length of the channel and are configured to detect a leading edge and a trailing edge of a volume of a fluid flowing though the channel. The sensors could be electrodes. The device could further have a binding surface on a portion of the wall of the channel and a detector in the vicinity of the wall of the channel, wherein the binding surface has molecules that bind to an analyte and the detector could be adapted to detect binding of the analyte to the binding surface.