发明申请
- 专利标题: Rapid and Low Cost Method for Isolating Nucleic Acid
- 专利标题(中): 快速低成本的分离核酸的方法
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申请号: US10577721申请日: 2004-10-20
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公开(公告)号: US20080166703A1公开(公告)日: 2008-07-10
- 发明人: Ralf Himmelreich , Sabine Werner , Claudia Fritz
- 申请人: Ralf Himmelreich , Sabine Werner , Claudia Fritz
- 申请人地址: DE Hilden
- 专利权人: QIAGEN GmbH
- 当前专利权人: QIAGEN GmbH
- 当前专利权人地址: DE Hilden
- 优先权: EP03025181.3 20031104
- 国际申请: PCT/EP04/11851 WO 20041020
- 主分类号: C12Q1/68
- IPC分类号: C12Q1/68 ; C07H1/00
摘要:
The invention relates to a rapid method for isolating nucleic acid from a nucleic acid source, wherein the nucleic acid source is lysed in the absence of a chaotropic salt and in the absence of an alcohol. The lysate is subsequently filtered through a porous matrix consisting of a material based on silica or of a silica coated material, which binds the nucleic acid in the absence of a chaotropic salt and in the absence of an alcohol. Finally, the nucleic acid is eluted from the porous matrix by an aqueous buffer solution. Furthermore, this invention relates to a test kit in order to isolate the nucleic acid.
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