公开(公告)号：US20070295244A1

公开(公告)日：2007-12-27

申请号：US11629990

申请日：2005-06-23

申请人： Ralf Himmelreich ,  Sabine Werner ,  Claudia Fritz ,  Hideo Takahashi ,  Masaki Shimojoh ,  Toshihiko Akamatsu ,  Keisuke Iriyama

发明人： Ralf Himmelreich ,  Sabine Werner ,  Claudia Fritz ,  Hideo Takahashi ,  Masaki Shimojoh ,  Toshihiko Akamatsu ,  Keisuke Iriyama

IPC分类号： C08K9/00 ,  C09C1/36 ,  C09C3/06

CPC分类号： C09C1/3692 ,  C01P2006/12 ,  C08K9/06 ,  C09C1/3661 ,  C09C1/3669 ,  C09C1/3684 ,  Y10T428/2993 ,  Y10T428/2995

摘要： The invention provides titanium dioxide pigments for plastics resin compositions which are excellent in dispersibility, light resistance, and weather resistance and little cause surface imperfection. In particular, the invention provides a titanium dioxide pigment suitable for general-purpose plastics resin compositions which can attain excellent dispersion and lacing resistance even when blended in a high concentration; a titanium dioxide pigment suitable for engineering plastics resin compositions which can attain dispersion properties, silver streaking resistance, inhibition of reduction in molecular weight, and thermal discoloration resistance at high levels; a process for the production of the pigments; and resin compositions containing the pigments. A titanium dioxide pigment for plastics resin compositions, characterized in that each titanium dioxide particle has a dense hydrous silica coat covering its surface and an organic compound coat formed on the silica coat not through an interlayer.

摘要翻译： 本发明提供了分散性，耐光性和耐候性优异且表面不完美的塑料树脂组合物用二氧化钛颜料。 特别地，本发明提供了适用于通用塑料树脂组合物的二氧化钛颜料，即使以高浓度混合也能获得优异的分散性和耐蕾曲性; 适用于工程塑料树脂组合物的二氧化钛颜料，其可以获得分散性能，银耐划伤性，抑制分子量降低和高水平的耐热变色性; 一种生产颜料的方法; 和含有颜料的树脂组合物。 一种用于塑料树脂组合物的二氧化钛颜料，其特征在于，每个二氧化钛颗粒具有覆盖其表面的致密含水二氧化硅涂层和在二氧化硅涂层上形成的有机复合涂层，而不是通过中间层。

公开(公告)号：US20080166703A1

公开(公告)日：2008-07-10

申请号：US10577721

申请日：2004-10-20

申请人： Ralf Himmelreich ,  Sabine Werner ,  Claudia Fritz

发明人： Ralf Himmelreich ,  Sabine Werner ,  Claudia Fritz

IPC分类号： C12Q1/68 ,  C07H1/00

CPC分类号： C12N15/1017 ,  C07H1/08

摘要： The invention relates to a rapid method for isolating nucleic acid from a nucleic acid source, wherein the nucleic acid source is lysed in the absence of a chaotropic salt and in the absence of an alcohol. The lysate is subsequently filtered through a porous matrix consisting of a material based on silica or of a silica coated material, which binds the nucleic acid in the absence of a chaotropic salt and in the absence of an alcohol. Finally, the nucleic acid is eluted from the porous matrix by an aqueous buffer solution. Furthermore, this invention relates to a test kit in order to isolate the nucleic acid.

摘要翻译： 本发明涉及从核酸源分离核酸的快速方法，其中核酸源在不存在离液序列盐的情况下裂解，并且在不存在醇的情况下裂解。 裂解物随后通过由基于二氧化硅或二氧化硅涂覆的材料的材料组成的多孔基质过滤，所述材料在不存在离液序列盐的情况下结合核酸，并且在不存在醇的情况下。 最后，通过缓冲液水溶液从多孔基质中洗脱核酸。 此外，本发明涉及用于分离核酸的测试试剂盒。

公开(公告)号：US08076069B2

公开(公告)日：2011-12-13

申请号：US12477813

申请日：2009-06-03

申请人： Christian Korfhage ,  Friederike Wilmer ,  Dirk Löffert ,  Ralf Himmelreich ,  Claudia Fritz ,  Kathleen Rieske

发明人： Christian Korfhage ,  Friederike Wilmer ,  Dirk Löffert ,  Ralf Himmelreich ,  Claudia Fritz ,  Kathleen Rieske

IPC分类号： C12Q1/68 ,  C07H21/00

CPC分类号： C12N1/06 ,  C12N15/1003 ,  C12Q1/6806 ,  G01N33/6803 ,  C12Q2531/113 ,  C12Q2527/137 ,  C12Q2527/125

摘要： The invention generally provides a method for the sample preparation for a subsequent preparation, processing or analysis method of a sample containing an at least one species of nucleic acid and/or one species of protein, whereby the method comprises the following steps: A) providing a sample which contains at least one species of a nucleic acid and/or of a protein, B) contacting the sample with a fluid or solid composition to produce a fluid sample preparation, whereby the composition contains at least a nitrogenous compound, which is selected from the group consisting of a) polyamines, b) amino acids, and oligo- and polypeptides, c) nitrogenous heterocyclic compounds, including homo- oder heteropolymers, which comprise these nitrogenous compounds, d) amines of the type R1R2NR3, whereby R1, R2 and R3 are chosen independently from one another from the group consisting of H, C1-C5-alkyl groups and aryl groups, whereby R1, R2 and R3 are not H simultaneously, e) carboxylic acid amides, f) inorganic ammonium salts, g) ammonium groups containing inner salt compounds, h) antibiotica binding nucleic acid, i) compounds which bind in the small cavity of the DNA, and mixtures of two or more of these compounds. The invention provides in particular a method for the lysis of a biological sample, and methods for the stabilization of biomolecules, a method for the decrease of inhibiting effects in a sample containing biomolecules and a differential masking method.

摘要翻译： 本发明通常提供了用于样品制备用于随后的含有至少一种核酸和/或一种蛋白质的样品的制备，加工或分析方法的方法，其中所述方法包括以下步骤：A）提供 包含至少一种核酸和/或蛋白质的样品，B）使样品与流体或固体组合物接触以产生流体样品制备物，其中所述组合物含有至少一种选自氮的化合物 包括a）多胺，b）氨基酸和寡聚和多肽，c）包含这些含氮化合物的含氮杂环化合物，包括均相杂聚物，d）R1R2NR3型胺，其中R1，R2 和R 3彼此独立地选自H，C 1 -C 5烷基和芳基，其中R 1，R 2和R 3不同时为H，e）羧酸酰胺，f ）无机铵盐，g）含有内盐化合物的铵基，h）抗生素结合核酸，i）结合在DNA的小腔中的化合物，以及两种或更多种这些化合物的混合物。 本发明特别提供了生物样品的裂解方法，以及用于稳定生物分子的方法，降低含有生物分子的样品中的抑制作用的方法和差示掩蔽法。

公开(公告)号：US20100323363A1

公开(公告)日：2010-12-23

申请号：US12862156

申请日：2010-08-24

申请人： Christian KORFHAGE ,  Friederike Wilmer ,  Dirk Löffert ,  Ralf Himmelreich ,  Claudia Fritz ,  Kathleen Rieske

发明人： Christian KORFHAGE ,  Friederike Wilmer ,  Dirk Löffert ,  Ralf Himmelreich ,  Claudia Fritz ,  Kathleen Rieske

IPC分类号： C12Q1/68

CPC分类号： C12N1/06 ,  C12N15/1003 ,  C12Q1/6806 ,  G01N33/6803 ,  C12Q2531/113 ,  C12Q2527/137 ,  C12Q2527/125

摘要： The invention generally provides a method for the sample preparation for a subsequent preparation, processing or analysis method of a sample containing an at least one species of nucleic acid and/or one species of protein, whereby the method comprises the following steps: A) providing a sample which contains at least one species of a nucleic acid and/or of a protein, B) contacting the sample with a fluid or solid composition to produce a fluid sample preparation, whereby the composition contains at least a nitrogenous compound, which is selected from the group consisting of a) polyamines, b) amino acids, and oligo- and polypeptides, c) nitrogenous heterocyclic compounds, including homo- oder heteropolymeres, which comprise these nitrogenous compounds, d) amines of the type R1R2NR3, whereby R1, R2 and R3 are chosen independently from one another from the group consisting of H, C1-C5-alkyl groups and aryl groups, whereby R1, R2 and R3 are not H simultaneously, e) carbonxylic acid amides, f) inorganic ammonium salts, g) ammonium groups containing inner salt compounds, h) antibiotica binding nucleic acid, i) compounds which bind in the small cavity of the DNA, and mixtures of two or more of these compounds. The invention provides in particular a method for the lysis of a biological sample, and methods for the stabilisation of biomolecules, a method for the decrease of inhibiting effects in a sample containing biomolecules and a differential masking method.

摘要翻译： 本发明通常提供了用于样品制备用于随后的含有至少一种核酸和/或一种蛋白质的样品的制备，加工或分析方法的方法，其中所述方法包括以下步骤：A）提供 包含至少一种核酸和/或蛋白质的样品，B）使样品与流体或固体组合物接触以产生流体样品制备物，其中所述组合物含有至少一种选自氮的化合物 来自由a）多胺，b）氨基酸和寡聚物和多肽组成的组，c）包含这些含氮化合物的含氮杂环化合物，包括均匀的杂多聚物，d）R1R2NR3型的胺，其中R1，R2 和R 3彼此独立地选自H，C 1 -C 5烷基和芳基，其中R 1，R 2和R 3不同时为H，e）碳酸酰胺， f）无机铵盐，g）含有内盐化合物的铵基，h）抗生素结合核酸，i）结合在DNA的小腔中的化合物，以及两种或更多种这些化合物的混合物。 本发明特别提供了生物样品的裂解方法，以及用于稳定生物分子的方法，降低含有生物分子的样品中的抑制作用的方法和差示掩蔽法。

公开(公告)号：US07838233B2

公开(公告)日：2010-11-23

申请号：US11910247

申请日：2006-04-01

申请人： Christian Korfhage ,  Friederike Wilmer ,  Dirk Löffert ,  Ralf Himmelreich ,  Claudia Fritz ,  Kathleen Rieske

发明人： Christian Korfhage ,  Friederike Wilmer ,  Dirk Löffert ,  Ralf Himmelreich ,  Claudia Fritz ,  Kathleen Rieske

IPC分类号： C12Q1/68 ,  C12P21/06 ,  C07K1/00 ,  C07K14/00 ,  C07H21/00 ,  C07H21/02 ,  C07H21/04

CPC分类号： C12N1/06 ,  C12N15/1003 ,  C12Q1/6806 ,  G01N33/6803 ,  C12Q2531/113 ,  C12Q2527/137 ,  C12Q2527/125

摘要： The invention generally provides a method for the sample preparation for a subsequent preparation, processing or analysis method of a sample containing an at least one species of nucleic acid and/or one species of protein, whereby the method comprises the following steps: A) providing a sample which contains at least one species of a nucleic acid and/or of a protein, B) contacting the sample with a fluid or solid composition to produce a fluid sample preparation, whereby the composition contains at least a nitrogenous compound, which is selected from the group consisting of a) polyamines, b) amino acids, and oligo- and polypeptides, c) nitrogenous heterocyclic compounds, including homo-older heteropolymeres, which comprise these nitrogenous compounds, d) amines of the type R1R2NR3, whereby R1, R2 and R3 are chosen independently from one another from the group consisting of H, C1-C5-alkyl groups and aryl groups, whereby R1, R2 and R3 are not H simultaneously, e) carboxylic acid amides, f) inorganic ammonium salts, g) ammonium groups containing inner salt compounds, h) antibiotica binding nucleic acid, i) compounds which bind in the small cavity of the DNA, and mixtures of two or more of these compounds. The invention provides In particular a method for the lysis of a biological sample, and methods for the stabilisation of biomolecules, a method for the decrease of inhibiting effects in a sample containing biomolecules and a differential masking method.

摘要翻译： 本发明通常提供了用于样品制备用于随后的含有至少一种核酸和/或一种蛋白质的样品的制备，加工或分析方法的方法，其中所述方法包括以下步骤：A）提供 包含至少一种核酸和/或蛋白质的样品，B）使样品与流体或固体组合物接触以产生流体样品制备物，其中所述组合物含有至少一种选自氮的化合物 来自由a）多胺，b）氨基酸和寡聚物和多肽组成的组，c）包含这些含氮化合物的含氮杂环化合物，包括同质杂多酸，d）R1R2NR3类型的胺，其中R1，R2 和R 3彼此独立地选自H，C 1 -C 5 - 烷基和芳基，其中R 1，R 2和R 3不同时为H，e）羧酸酰胺， f）无机铵盐，g）含有内盐化合物的铵基，h）抗生素结合核酸，i）结合在DNA的小腔中的化合物，以及两种或更多种这些化合物的混合物。 本发明特别提供一种用于裂解生物样品的方法，以及用于稳定生物分子的方法，降低含有生物分子的样品中的抑制作用的方法和差示掩蔽法。

公开(公告)号：US08785120B2

公开(公告)日：2014-07-22

申请号：US12862156

申请日：2010-08-24

申请人： Christian Korfhage ,  Friederike Wilmer ,  Dirk Loffert ,  Ralf Himmelreich ,  Claudia Fritz ,  Kathleen Rieske

发明人： Christian Korfhage ,  Friederike Wilmer ,  Dirk Loffert ,  Ralf Himmelreich ,  Claudia Fritz ,  Kathleen Rieske

IPC分类号： C12Q1/68 ,  G01N33/53 ,  C07H21/04

CPC分类号： C12N1/06 ,  C12N15/1003 ,  C12Q1/6806 ,  G01N33/6803 ,  C12Q2531/113 ,  C12Q2527/137 ,  C12Q2527/125

摘要： The invention generally provides a method for the sample preparation for a subsequent preparation, processing or analysis method of a sample containing an at least one species of nucleic acid and/or one species of protein, whereby the method comprises the following steps: A) providing a sample which contains at least one species of a nucleic acid and/or of a protein, B) contacting the sample with a fluid or solid composition to produce a fluid sample preparation, whereby the composition contains at least a nitrogenous compound, which is selected from the group consisting of a) polyamines, b) amino acids, and oligo- and polypeptides, c) nitrogenous heterocyclic compounds, including homo-oder heteropolymeres, which comprise these nitrogenous compounds, d) amines of the type R1R2NR3, whereby R1, R2 and R3 are chosen independently from one another from the group consisting of H, C1-C5-alkyl groups and aryl groups, whereby R1, R2 and R3 are not H simultaneously, e) carbonxylic acid amides, f) inorganic ammonium salts, g) ammonium groups containing inner salt compounds, h) antibiotica binding nucleic acid, i) compounds which bind in the small cavity of the DNA, and mixtures of two or more of these compounds. The invention provides in particular a method for the lysis of a biological sample, and methods for the stabilization of biomolecules, a method for the decrease of inhibiting effects in a sample containing biomolecules and a differential masking method.

摘要翻译： 本发明通常提供了用于样品制备用于随后的含有至少一种核酸和/或一种蛋白质的样品的制备，加工或分析方法的方法，其中所述方法包括以下步骤：A）提供 包含至少一种核酸和/或蛋白质的样品，B）使样品与流体或固体组合物接触以产生流体样品制备物，其中所述组合物含有至少一种选自氮的化合物 由以下组成的组：a）多胺，b）氨基酸以及寡聚和多肽，c）包含这些含氮化合物的含氮杂环化合物，包括同质杂多糖，d）R1R2NR3类型的胺，其中R1，R2 和R 3彼此独立地选自H，C 1 -C 5烷基和芳基，其中R 1，R 2和R 3不同时为H，e）碳酸酰胺， f）无机铵盐，g）含有内盐化合物的铵基，h）抗生素结合核酸，i）结合在DNA的小腔中的化合物，以及两种或更多种这些化合物的混合物。 本发明特别提供了生物样品的裂解方法，以及用于稳定生物分子的方法，降低含有生物分子的样品中的抑制作用的方法和差示掩蔽法。

公开(公告)号：US20080187924A1

公开(公告)日：2008-08-07

申请号：US11910247

申请日：2006-04-01

申请人： Christian Korfhage ,  Friederike Wilmer ,  Dirk Loffert ,  Ralf Himmelreich ,  Claudia Fritz

发明人： Christian Korfhage ,  Friederike Wilmer ,  Dirk Loffert ,  Ralf Himmelreich ,  Claudia Fritz

IPC分类号： C12Q1/68

CPC分类号： C12N1/06 ,  C12N15/1003 ,  C12Q1/6806 ,  G01N33/6803 ,  C12Q2531/113 ,  C12Q2527/137 ,  C12Q2527/125

摘要： The invention generally provides a method for the sample preparation for a subsequent preparation, processing or analysis method of a sample containing an at least one species of nucleic acid and/or one species of protein, whereby the method comprises the following steps: A) providing a sample which contains at least one species of a nucleic acid and/or of a protein, B) contacting the sample with a fluid or solid composition to produce a fluid sample preparation, whereby the composition contains at least a nitrogenous compound, which is selected from the group consisting of a) polyamines, b) amino acids, and oligo- and polypeptides, c) nitrogenous heterocyclic compounds, including homo-older heteropolymeres, which comprise these nitrogenous compounds, d) amines of the type R1R2NR3, whereby R1, R2 and R3 are chosen independently from one another from the group consisting of H, C1-C5-alkyl groups and aryl groups, whereby R1, R2 and R3 are not H simultaneously, e) carboxylic acid amides, f) inorganic ammonium salts, g) ammonium groups containing inner salt compounds, h) antibiotica binding nucleic acid, i) compounds which bind in the small cavity of the DNA, and mixtures of two or more of these compounds. The invention provides In particular a method for the lysis of a biological sample, and methods for the stabilisation of biomolecules, a method for the decrease of inhibiting effects in a sample containing biomolecules and a differential masking method.

摘要翻译： 本发明通常提供了用于样品制备用于随后的含有至少一种核酸和/或一种蛋白质的样品的制备，加工或分析方法的方法，其中所述方法包括以下步骤：A）提供 包含至少一种核酸和/或蛋白质的样品，B）使样品与流体或固体组合物接触以产生流体样品制备物，其中所述组合物含有至少一种选自氮的化合物 来自由a）多胺，b）氨基酸和寡肽和多肽组成的组，c）包含这些含氮化合物的含氮杂环化合物，包括同质杂多酸，d）R 1型胺， 其中R 1，R 2，R 3，R 3，R 3，R 3， SUP>彼此独立地选自由H，C 1 -C 5 - 烷基和 芳基，其中R 1，R 2和R 3不同时为H，e）羧酸酰胺，f）无机铵盐， g）含有内盐化合物的铵基，h）抗生素结合核酸，i）结合在DNA的小腔中的化合物，以及两种或更多种这些化合物的混合物。 本发明特别提供一种用于裂解生物样品的方法，以及用于稳定生物分子的方法，降低含有生物分子的样品中的抑制作用的方法和差示掩蔽法。

公开(公告)号：US20070264500A1

公开(公告)日：2007-11-15

申请号：US11547156

申请日：2005-03-22

申请人： Claudia Fritz

发明人： Claudia Fritz

IPC分类号： D02G3/00 ,  B28B3/00

CPC分类号： D01F6/06 ,  D01F6/46 ,  Y10T428/2913

摘要： The invention relates to polypropylene-based monofilaments having improved properties, especially an improved abrasion resistance, to a process for manufacturing these monofilaments and to their use. The invention relates more particularly to polypropylene-based monofilaments, the polypropylene being obtained by polymerizing propylene in the presence of a metallocene catalyst.

摘要翻译： 本发明涉及对制造这些单丝的方法及其用途具有改进的性能，特别是改进的耐磨性的聚丙烯基单丝。 本发明更具体地涉及基于聚丙烯的单丝，聚丙烯是通过在茂金属催化剂存在下聚合丙烯获得的。