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发明申请
US20080187916A1 Identification of cell culture contaminants among Mollicutes species by a PCR based assay 失效
通过基于PCR的测定法鉴定Mollicutes物种中的细胞培养物污染物

Identification of cell culture contaminants among Mollicutes species by a PCR based assay
摘要：
The present invention encompasses nucleic acids, methods, compositions, and kits for sensitive, rapid and specific detection of Mycoplasma, Acholeplasma, Ureaplasma, Phytoplasma and Spiroplasma species in a sample. The invention utilizes specific primers and amplification methods that permit differentiation between species due to specific amplification of target nucleic acids of contaminating Mollicute cells. In one embodiment, the invention utilizes the differing melting temperatures (Tm) of various potential PCR products to identify whether they are specific target amplification products, non-specific, non-target amplification products, specific positive control products, or primer-dimer products.
摘要（中）：

本发明包括用于在样品中敏感，快速和特异性检测支原体，孔雀斑，解纤维原体，植原体和螺旋体物种的核酸，方法，组合物和试剂盒。 本发明利用特异性引物和扩增方法，其允许由于污染的Mollicute细胞的靶核酸的特异性扩增而在物种之间进行分化。 在一个实施方案中，本发明利用各种潜在PCR产物的不同的融合温度（Tm）来鉴定它们是特异性靶向扩增产物，非特异性，非靶扩增产物，特异性阳性对照产物或引物二聚体产物。

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