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    Identification of cell culture contaminants among Mollicutes species by a PCR based assay
    1.
    发明申请
    Identification of cell culture contaminants among Mollicutes species by a PCR based assay 失效
    通过基于PCR的测定法鉴定Mollicutes物种中的细胞培养物污染物

    公开(公告)号：US20080187916A1

    公开(公告)日：2008-08-07

    申请号：US11702615

    申请日：2007-02-06

    申请人： Pranvera Ikonomi ,  Deborah Polayes ,  Karin Cottrill ,  Quanyi Li

    发明人： Pranvera Ikonomi ,  Deborah Polayes ,  Karin Cottrill ,  Quanyi Li

    IPC分类号： C12Q1/68 ,  C12N15/31

    CPC分类号： C07K14/30 ,  C12Q1/689 ,  C12Q2600/16 ,  C12Q2600/166

    摘要： The present invention encompasses nucleic acids, methods, compositions, and kits for sensitive, rapid and specific detection of Mycoplasma, Acholeplasma, Ureaplasma, Phytoplasma and Spiroplasma species in a sample. The invention utilizes specific primers and amplification methods that permit differentiation between species due to specific amplification of target nucleic acids of contaminating Mollicute cells. In one embodiment, the invention utilizes the differing melting temperatures (Tm) of various potential PCR products to identify whether they are specific target amplification products, non-specific, non-target amplification products, specific positive control products, or primer-dimer products.

    摘要翻译： 本发明包括用于在样品中敏感，快速和特异性检测支原体，孔雀斑，解纤维原体，植原体和螺旋体物种的核酸，方法，组合物和试剂盒。 本发明利用特异性引物和扩增方法，其允许由于污染的Mollicute细胞的靶核酸的特异性扩增而在物种之间进行分化。 在一个实施方案中，本发明利用各种潜在PCR产物的不同的融合温度（Tm）来鉴定它们是特异性靶向扩增产物，非特异性，非靶扩增产物，特异性阳性对照产物或引物二聚体产物。

    Method for detecting the presence of mammalian organisms using specific cytochrome c oxidase I (COI) and/or cytochrome b subsequences by a PCR based assay
    5.
    发明申请
    Method for detecting the presence of mammalian organisms using specific cytochrome c oxidase I (COI) and/or cytochrome b subsequences by a PCR based assay 审中-公开
    使用特异性细胞色素c氧化酶I（COI）和/或细胞色素b亚序列通过基于PCR的检测法检测哺乳动物生物体的存在的方法

    公开(公告)号：US20080113349A1

    公开(公告)日：2008-05-15

    申请号：US11592117

    申请日：2006-11-03

    申请人： Pranvera Ikonomi ,  Jason Cooper ,  Greg Sykes ,  Yvonne Reid

    发明人： Pranvera Ikonomi ,  Jason Cooper ,  Greg Sykes ,  Yvonne Reid

    IPC分类号： C12Q1/68 ,  C07H21/00 ,  C12Q1/00

    CPC分类号： C12Q1/689 ,  C12Q1/6881 ,  C12Q2600/16

    摘要： The present invention relates to a PCR (polymerase chain reaction) based assay that is useful for detecting, identifying, quantitating and analysis of a target nucleic acid (target nucleic acid hereinafter) in a sample. More specifically, the present invention relates to a PCR based assay that can improve accuracy in detecting, identifying, and quantitating contamination in mammalian cell lines using a PCR-based assay of nucleic acid oligonucleotides (oligoprobes) having a sequence expected to be complementary to a target nucleic acid sequence in a sample. More specifically, the sample may contain either cells or nucleic acid isolated from a cell line. The present invention also relates to a detection kit using the PCR-based assay. The invention also involves a method for using specifically produced nucleic acids complementary to specific sequences or populations of different sequences of the cytochrome c oxidase I (COI) and/or cytochrome b, to detect, identify, and quantify specific organisms, groups of organisms, groups of eukaryotic cells or viruses in cells.

    摘要翻译： 本发明涉及可用于检测，鉴定，定量和分析样品中靶核酸（下文中的靶核酸）的基于PCR（聚合酶链式反应）的测定法。 更具体地，本发明涉及基于PCR的测定法，其可以使用具有期望与其互补的序列的核酸寡核苷酸（寡核苷酸）的基于PCR的测定来提高检测，鉴定和定量哺乳动物细胞系中的污染的准确性 靶核酸序列。 更具体地，样品可以含有从细胞系分离的细胞或核酸。 本发明还涉及使用基于PCR的测定法的检测试剂盒。 本发明还涉及使用特异性产生的与细胞色素c氧化酶I（COI）和/或细胞色素b的不同序列的特定序列或群体互补的核酸来检测，鉴定和定量特定生物体，生物体组， 细胞中的真核细胞或病毒组。

    Identification of cell culture contaminants among Mollicutes species by a PCR based assay
    6.
    发明授权
    Identification of cell culture contaminants among Mollicutes species by a PCR based assay 失效
    通过基于PCR的测定法鉴定Mollicutes物种中的细胞培养物污染物

    公开(公告)号：US07872116B2

    公开(公告)日：2011-01-18

    申请号：US11702615

    申请日：2007-02-06

    申请人： Pranvera Ikonomi ,  Deborah Polayes ,  Karin Cottrill ,  Quanyi Li

    发明人： Pranvera Ikonomi ,  Deborah Polayes ,  Karin Cottrill ,  Quanyi Li

    IPC分类号： C07H21/04 ,  C12Q1/68 ,  C12P19/34

    CPC分类号： C07K14/30 ,  C12Q1/689 ,  C12Q2600/16 ,  C12Q2600/166

    摘要： The present invention encompasses nucleic acids, methods, compositions, and kits for sensitive, rapid and specific detection of Mycoplasma, Acholeplasma, Ureaplasma, Phytoplasma and Spiroplasma species in a sample. The invention utilizes specific primers and amplification methods that permit differentiation between species due to specific amplification of target nucleic acids of contaminating Mollicute cells. In one embodiment, the invention utilizes the differing melting temperatures (Tm) of various potential PCR products to identify whether they are specific target amplification products, non-specific, non-target amplification products, specific positive control products, or primer-dimer products.

    摘要翻译： 本发明包括用于在样品中敏感，快速和特异性检测支原体，孔雀斑，解纤维原体，植原体和螺旋体物种的核酸，方法，组合物和试剂盒。 本发明利用特异性引物和扩增方法，其允许由于污染的Mollicute细胞的靶核酸的特异性扩增而在物种之间进行分化。 在一个实施方案中，本发明利用各种潜在PCR产物的不同的融合温度（Tm）来鉴定它们是特异性靶向扩增产物，非特异性，非靶扩增产物，特异性阳性对照产物或引物二聚体产物。