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    Methods and compositions for enhancing the delivery of a nucleic acid to a cell
    1.
    发明申请
    Methods and compositions for enhancing the delivery of a nucleic acid to a cell 审中-公开
    用于增强核酸向细胞递送的方法和组合物

    公开(公告)号：US20050080030A1

    公开(公告)日：2005-04-14

    申请号：US10656068

    申请日：2003-09-05

    申请人： Robert Levy ,  Peter Jones ,  Quanyi Li

    发明人： Robert Levy ,  Peter Jones ,  Quanyi Li

    IPC分类号： C12N15/09 ,  A61K31/7088 ,  A61K38/00 ,  A61K48/00 ,  A61P43/00 ,  C12N15/87 ,  C12N15/85

    CPC分类号： C12N15/87

    摘要： The present invention relates to methods and compositions useful for enhancing the efficiency of delivery of a nucleic acid to a cell. Preferably the cell is a mammalian cell. The method comprises providing to a cell an agent capable of enhancing the cytoskeletal permissiveness of the cell for transfection. The method also comprises providing to the cell a nucleic acid delivery system for the transfection of the cell, whereby the efficiency of delivery of a nucleic acid to the cell is enhanced. Compositions and kits for enhancing the efficiency of delivery of a nucleic acid to a cell are also included

    摘要翻译： 本发明涉及可用于增强核酸向细胞递送效率的方法和组合物。 优选地，细胞是哺乳动物细胞。 该方法包括向细胞提供能够增强用于转染的细胞的细胞骨架容许度的试剂。 该方法还包括向细胞提供用于转染细胞的核酸递送系统，从而提高核酸递送至细胞的效率。 还包括用于提高将核酸递送至细胞的效率的组合物和试剂盒

    Identification of cell culture contaminants among Mollicutes species by a PCR based assay
    2.
    发明申请
    Identification of cell culture contaminants among Mollicutes species by a PCR based assay 失效
    通过基于PCR的测定法鉴定Mollicutes物种中的细胞培养物污染物

    公开(公告)号：US20080187916A1

    公开(公告)日：2008-08-07

    申请号：US11702615

    申请日：2007-02-06

    申请人： Pranvera Ikonomi ,  Deborah Polayes ,  Karin Cottrill ,  Quanyi Li

    发明人： Pranvera Ikonomi ,  Deborah Polayes ,  Karin Cottrill ,  Quanyi Li

    IPC分类号： C12Q1/68 ,  C12N15/31

    CPC分类号： C07K14/30 ,  C12Q1/689 ,  C12Q2600/16 ,  C12Q2600/166

    摘要： The present invention encompasses nucleic acids, methods, compositions, and kits for sensitive, rapid and specific detection of Mycoplasma, Acholeplasma, Ureaplasma, Phytoplasma and Spiroplasma species in a sample. The invention utilizes specific primers and amplification methods that permit differentiation between species due to specific amplification of target nucleic acids of contaminating Mollicute cells. In one embodiment, the invention utilizes the differing melting temperatures (Tm) of various potential PCR products to identify whether they are specific target amplification products, non-specific, non-target amplification products, specific positive control products, or primer-dimer products.

    摘要翻译： 本发明包括用于在样品中敏感，快速和特异性检测支原体，孔雀斑，解纤维原体，植原体和螺旋体物种的核酸，方法，组合物和试剂盒。 本发明利用特异性引物和扩增方法，其允许由于污染的Mollicute细胞的靶核酸的特异性扩增而在物种之间进行分化。 在一个实施方案中，本发明利用各种潜在PCR产物的不同的融合温度（Tm）来鉴定它们是特异性靶向扩增产物，非特异性，非靶扩增产物，特异性阳性对照产物或引物二聚体产物。

    Identification of cell culture contaminants among Mollicutes species by a PCR based assay
    3.
    发明授权
    Identification of cell culture contaminants among Mollicutes species by a PCR based assay 失效
    通过基于PCR的测定法鉴定Mollicutes物种中的细胞培养物污染物

    公开(公告)号：US07872116B2

    公开(公告)日：2011-01-18

    申请号：US11702615

    申请日：2007-02-06

    申请人： Pranvera Ikonomi ,  Deborah Polayes ,  Karin Cottrill ,  Quanyi Li

    发明人： Pranvera Ikonomi ,  Deborah Polayes ,  Karin Cottrill ,  Quanyi Li

    IPC分类号： C07H21/04 ,  C12Q1/68 ,  C12P19/34

    CPC分类号： C07K14/30 ,  C12Q1/689 ,  C12Q2600/16 ,  C12Q2600/166

    摘要： The present invention encompasses nucleic acids, methods, compositions, and kits for sensitive, rapid and specific detection of Mycoplasma, Acholeplasma, Ureaplasma, Phytoplasma and Spiroplasma species in a sample. The invention utilizes specific primers and amplification methods that permit differentiation between species due to specific amplification of target nucleic acids of contaminating Mollicute cells. In one embodiment, the invention utilizes the differing melting temperatures (Tm) of various potential PCR products to identify whether they are specific target amplification products, non-specific, non-target amplification products, specific positive control products, or primer-dimer products.

    摘要翻译： 本发明包括用于在样品中敏感，快速和特异性检测支原体，孔雀斑，解纤维原体，植原体和螺旋体物种的核酸，方法，组合物和试剂盒。 本发明利用特异性引物和扩增方法，其允许由于污染的Mollicute细胞的靶核酸的特异性扩增而在物种之间进行分化。 在一个实施方案中，本发明利用各种潜在PCR产物的不同的融合温度（Tm）来鉴定它们是特异性靶向扩增产物，非特异性，非靶扩增产物，特异性阳性对照产物或引物二聚体产物。