发明申请
- 专利标题: Methods and compositions for RNA synthesis
- 专利标题(中): 用于RNA合成的方法和组合物
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申请号: US11713424申请日: 2007-02-28
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公开(公告)号: US20080206851A1公开(公告)日: 2008-08-28
- 发明人: Douglas J. Dellinger , Geraldine F. Dellinger , Marvin H. Caruthers
- 申请人: Douglas J. Dellinger , Geraldine F. Dellinger , Marvin H. Caruthers
- 主分类号: C07H21/02
- IPC分类号: C07H21/02 ; C12M1/34
摘要:
In some embodiments, the present disclosure relates to new phosphoramidite compositions, that have Silyl-containing carbonate or thiocarbonate or ether as 5′-hydroxyl protecting groups useful fo the syntheis of RNA, and in particular for the synthesis of long sequences of RNA (e.g., >50 mer). In some embodiments, there are provided methods for simultaneous oxidation of the internucleoside phosphate triester linkages and removal of the 5′-hydroxyl-protecting group, making this process a new 2-step RNA synthesis, that involves the use of peroxyanions in combination with fluoride anions.