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发明申请
US20080261206A1 Oligonucleotide for Detection of a Microorganism, Diagnostic Kits and Methods for Detection of Microorganisms Using the Oligonucleotide 审中-公开
用于检测微生物的寡核苷酸，诊断试剂盒和使用寡核苷酸检测微生物的方法

Oligonucleotide for Detection of a Microorganism, Diagnostic Kits and Methods for Detection of Microorganisms Using the Oligonucleotide
摘要：
The present invention relates to a method so called Bacterial Digitalcode System (BaDis) that identifies microorganism by using bacterial-specific, genus-specific and species-specific oligonucleotides from a variety of samples or specimens for detection and differential diagnosis of microorganism. Particularly, the present invention relates to bacterial-specific, genus-specific and species-specific oligonucleotides designed by the target nucleotide sequences of 23S rDNA or ITS gene, polymerase chain reaction (hereinafter, referred to as “PCR”) kits using the oligonucleotides as a primer, the microarray containing the oligonucleotides as a probe, and methods for detecting microorganism by using the oligonucleotides. Therefore, the present invention can be applied to detect the presence of microorganism and diagnose differentially all microorganism such as pathogenic bacteria of infectious diseases, bacteria inducing food poisoning, bacteria contaminating biomedical products and environmental pollutants.
摘要（中）：

本发明涉及一种所谓的细菌数字代码系统（BaDis）的方法，其通过使用来自各种样品或样品的细菌特异性，属特异性和物种特异性寡核苷酸来鉴定微生物，用于微生物的鉴定和鉴别诊断。 特别地，本发明涉及由23S rDNA或ITS基因的靶核苷酸序列设计的细菌特异性，属特异性和物种特异性寡核苷酸，使用寡核苷酸作为聚合酶链反应（以下称为“PCR”）试剂盒 引物，含有寡核苷酸作为探针的微阵列，以及通过使用寡核苷酸检测微生物的方法。 因此，本发明可以应用于检测微生物的存在并差异地诊断所有微生物，如感染性疾病的病原菌，诱导食物中毒的细菌，细菌污染的生物医学产品和环境污染物。

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