公开(公告)号：US08219151B2

公开(公告)日：2012-07-10

申请号：US12403138

申请日：2009-03-12

申请人： Hee Kyung Park ,  Jong Hwan Kim ,  Seon Hwi Cho

发明人： Hee Kyung Park ,  Jong Hwan Kim ,  Seon Hwi Cho

IPC分类号： H04M1/00 ,  G06F3/01 ,  G06F3/041

CPC分类号： H04M1/72583 ,  H04M1/72552 ,  H04M19/047 ,  H04M2250/22 ,  H04M2250/52

摘要： A portable terminal is provided that includes a vibration generator for vibrating the portable terminal, a receiving device for receiving input from a user and determining a coordinate value on a display screen based on the received input, and a controller for determining a vibration pattern corresponding to information on a display screen based on the determined coordinate value.

摘要翻译： 提供一种便携式终端，其包括用于振动便携式终端的振动发生器，用于接收来自用户的输入并基于所接收的输入来确定显示屏上的坐标值的接收装置，以及用于确定对应于 基于确定的坐标值在显示屏幕上的信息。

公开(公告)号：US08133985B2

公开(公告)日：2012-03-13

申请号：US12300235

申请日：2007-12-14

申请人： Hyunil Lee ,  Hee Kyung Park ,  Bong Ho Um ,  Serka Kim ,  Jae Jin Choi ,  Jung Hyun Min ,  Hye Yeon Kim

发明人： Hyunil Lee ,  Hee Kyung Park ,  Bong Ho Um ,  Serka Kim ,  Jae Jin Choi ,  Jung Hyun Min ,  Hye Yeon Kim

IPC分类号： C07K2/00 ,  C07K1/00 ,  C40B80/00 ,  C40B40/06

CPC分类号： C07K14/003 ,  A61K47/641 ,  Y10S977/754

摘要： The present invention relates to a peptide nucleic acid (PNA) conjugated with multi-amine linkers, and a method to prepare the same and utilization thereof. More specifically, the method is characterized by conjugating monomers having multi-amine functionality sequentially at a PNA terminal, and effectively immobilizing the PNA conjugated with multi-amine linkers on a solid surface. A PNA array prepared using the PNA conjugated with multi-amine linkers exhibits improved sensitivity and specificity of signals for detecting target nucleic acids as compared to a PNA array using PNA probes having only one amine group. The PNA conjugated with multi-amine linkers can be utilized in nucleic acid detecting devices or kits for gene diagnosis such as PNA microarrays, PNA chips, PNA field-effect transistors and impedance detectors.

摘要翻译： 本发明涉及与多胺接头共轭的肽核酸（PNA）及其制备方法及应用。 更具体地说，该方法的特征在于在PNA末端连续连接具有多胺官能团的单体，并有效地将与多胺接头共轭的PNA固定在固体表面上。 使用与多胺接头共轭的PNA制备的PNA阵列与使用仅具有一个胺基团的PNA探针的PNA阵列相比，显示出用于检测靶核酸的信号的改善的灵敏度和特异性。 与多胺接头结合的PNA可用于核酸检测装置或用于基因诊断的试剂盒，例如PNA微阵列，PNA芯片，PNA场效应晶体管和阻抗检测器。

公开(公告)号：US20100248980A1

公开(公告)日：2010-09-30

申请号：US12745857

申请日：2008-12-04

申请人： Hee Kyung Park ,  Jae Jin Choi

发明人： Hee Kyung Park ,  Jae Jin Choi

IPC分类号： C40B30/04 ,  C40B50/18 ,  C40B70/00

CPC分类号： C12Q1/6837 ,  C12Q2525/107 ,  G01N33/532 ,  G01N2333/9015 ,  G01N2333/9127 ,  C12Q2565/537 ,  C12Q2521/131 ,  C12Q2521/501

摘要： Disclosed are a method for selective labeling of target nucleic acids on an array having nucleic acid analogue, e.g. PNA (peptide nucleic acid), probes immobilized on a support or supports, comprising adding to the array a detectable label and an agent for introducing the label into the target nucleic acids, after hybridization between the target nucleic acids and the nucleic acid analogue probes, and a method for detection of target nucleic acids using the same.

摘要翻译： 公开了在具有核酸类似物的阵列上选择性标记靶核酸的方法，例如， PNA（肽核酸），固定在载体或载体上的探针，包括在靶核酸和核酸类似物探针杂交后，向阵列中加入可检测标记和引入标记物到靶核酸中的试剂， 以及使用其的靶核酸的检测方法。

公开(公告)号：US20090312197A1

公开(公告)日：2009-12-17

申请号：US12300235

申请日：2007-12-14

申请人： Hyunil Lee ,  Hee Kyung Park ,  Bong Ho Um ,  Serka Kim ,  Jae Jin Choi ,  Jung Hyun Min ,  Hye Yeon Kim

发明人： Hyunil Lee ,  Hee Kyung Park ,  Bong Ho Um ,  Serka Kim ,  Jae Jin Choi ,  Jung Hyun Min ,  Hye Yeon Kim

IPC分类号： C40B40/08 ,  C07K9/00 ,  C07K1/00 ,  C40B50/14

CPC分类号： C07K14/003 ,  A61K47/641 ,  Y10S977/754

摘要： The present invention relates to a peptide nucleic acid (PNA) conjugated with multi-amine linkers, and a method to prepare the same and utilization thereof. More specifically, the method is characterized by conjugating monomers having multi-amine functionality sequentially at a PNA terminal, and effectively immobilizing the PNA conjugated with multi-amine linkers on a solid surface. A PNA array prepared using the PNA conjugated with multi-amine linkers exhibits improved sensitivity and specificity of signals for detecting target nucleic acids as compared to a PNA array using PNA probes having only one amine group. The PNA conjugated with multi-amine linkers can be utilized in nucleic acid detecting devices or kits for gene diagnosis such as PNA microarrays, PNA chips, PNA field-effect transistors and impedance detectors.

摘要翻译： 本发明涉及与多胺接头共轭的肽核酸（PNA）及其制备方法及其应用。 更具体地说，该方法的特征在于在PNA末端连续连接具有多胺官能团的单体，并有效地将与多胺接头共轭的PNA固定在固体表面上。 使用与多胺接头共轭的PNA制备的PNA阵列与使用仅具有一个胺基团的PNA探针的PNA阵列相比，显示出用于检测靶核酸的信号的改善的灵敏度和特异性。 与多胺接头结合的PNA可用于核酸检测装置或用于基因诊断的试剂盒，例如PNA微阵列，PNA芯片，PNA场效应晶体管和阻抗检测器。

公开(公告)号：US20080233557A1

公开(公告)日：2008-09-25

申请号：US11860983

申请日：2007-09-25

申请人： Hee Kyung PARK ,  Hyunil LEE ,  Jae Jin CHOI ,  Serka KIM

发明人： Hee Kyung PARK ,  Hyunil LEE ,  Jae Jin CHOI ,  Serka KIM

IPC分类号： C12Q1/70 ,  C07H21/04

CPC分类号： C12Q1/706 ,  C12Q1/6883 ,  C12Q2600/106 ,  C12Q2600/156

摘要： Disclosed are peptide nucleic acid (PNA) probes to detect lamivudine resistant mutants of hepatitis B virus (HBV), which causes acute and chronic hepatitis, kits for detecting lamivudine resistant HBV comprising the PNA probes, and methods for detecting lamivudine resistant HBV by using the kits. They can accurately detect mutations of rtL180M, rtM204V, rtM204I and rtV2071 within B and C domains of HBV DNA polymerase gene, the main cause of lamivudine resistance, as well as mixed mutants of more than one mutant. They can detect lamivudine resistant HBV with high specificity and sensitivity.

摘要翻译： 公开了用于检测引起急性和慢性肝炎的乙型肝炎病毒（HBV）的拉米夫定抗性突变体的肽核酸（PNA）探针，用于检测包含PNA探针的拉米夫定耐药性HBV的试剂盒，以及通过使用 工具包 他们可以准确检测HBV DNA聚合酶基因的B和C结构域中rtL180M，rtM204V，rtM204I和rtV2071的突变，这是拉米夫定耐药的主要原因，以及多个突变体的混合突变体。 他们可以检测出具有高特异性和灵敏度的拉米夫定耐药HBV。

公开(公告)号：US20100240058A1

公开(公告)日：2010-09-23

申请号：US12741413

申请日：2008-11-24

申请人： Hee Kyung Park ,  Su Young Oh

发明人： Hee Kyung Park ,  Su Young Oh

IPC分类号： C12Q1/68 ,  C07K9/00 ,  C12N5/02

CPC分类号： C07K14/003 ,  C12N15/113 ,  C12N2310/113 ,  C12N2310/3181 ,  C12N2310/3513 ,  C12Q1/6897 ,  C12Q2525/207

摘要： Disclosed are a microRNA antisense PNA capable of inhibiting the activity or function of microRNA, a composition for inhibiting the activity or function of microRNA containing the same, a method for inhibiting the activity or function of microRNA using the same, and a method for evaluating the effectiveness thereof.

摘要翻译： 公开了能够抑制微小RNA的活性或功能的微小RNA反义PNA，用于抑制其含有的微小RNA的活性或功能的组合物，使用其的微小RNA的抑制活性或功能的方法，以及评估方法 有效性。

公开(公告)号：US20100004028A1

公开(公告)日：2010-01-07

申请号：US12403138

申请日：2009-03-12

申请人： Hee Kyung Park ,  Jong Hwan Kim ,  Seon Hwi Cho

发明人： Hee Kyung Park ,  Jong Hwan Kim ,  Seon Hwi Cho

IPC分类号： H04M1/00

CPC分类号： H04M1/72583 ,  H04M1/72552 ,  H04M19/047 ,  H04M2250/22 ,  H04M2250/52

摘要： A portable terminal is provided that includes a vibration generator for vibrating the portable terminal, a receiving device for receiving input from a user and determining a coordinate value on a display screen based on the received input, and a controller for determining a vibration pattern corresponding to information on a display screen based on the determined coordinate value.

摘要翻译： 提供一种便携式终端，其包括用于振动便携式终端的振动发生器，用于接收来自用户的输入并基于所接收的输入来确定显示屏上的坐标值的接收装置，以及用于确定对应于 基于确定的坐标值在显示屏幕上的信息。

公开(公告)号：US20080248461A1

公开(公告)日：2008-10-09

申请号：US11851581

申请日：2007-09-07

申请人： Hee Kyung Park ,  Hyunil Lee ,  Jae Jin Choi ,  Serka Kim

发明人： Hee Kyung Park ,  Hyunil Lee ,  Jae Jin Choi ,  Serka Kim

IPC分类号： C12Q1/70 ,  C07H21/00

CPC分类号： C12Q1/708 ,  C12Q2525/107

摘要： Disclosed are PNA probes capable of genotype specifically binding with Human Paillomavirus (HPV) DNA, kits for detecting HPV genotypes comprising the probes, and methods for detecting HPV genotypes by using the kits, which enables the accurate detection of all 24 genotypes of HPV found in cervix, diagnosis of combined infection with more than one HPV genotype, and detection of HPV genotypes with high specificity and sensitivity.

摘要翻译： 公开了能够基因型与人类苍蝇病毒（HPV）DNA特异性结合的PNA探针，用于检测包含探针的HPV基因型的试剂盒，以及通过使用试剂盒检测HPV基因型的方法，其能够精确检测所有24种HPV基因型 子宫颈，联合感染多种HPV基因型的诊断，以及具有高特异性和敏感性的HPV基因型的检测。

公开(公告)号：US20060134792A1

公开(公告)日：2006-06-22

申请号：US10567072

申请日：2004-08-02

申请人： Cheol-Min Kim ,  Hee-Kyung Park ,  Mong Cho ,  Hyun-Jung Jang ,  Jeong Heo

发明人： Cheol-Min Kim ,  Hee-Kyung Park ,  Mong Cho ,  Hyun-Jung Jang ,  Jeong Heo

IPC分类号： C12Q1/00

CPC分类号： C12Q1/706 ,  C12Q1/6837 ,  C12Q2545/113

摘要： Provided are a microarray manufactured using a mixture of target probes for drug-resistant HBV detection, quality control probes for controlling quality in probe hybridization and fabrication of microarrays, and negative control probes for determining the presence and ratio of more than one type, i.e., a wild type and a mutant in a codon, measuring a background of non-specific cross-hybridization, and discriminating homozygotes and heterozygotes, and a method of detecting a drug-resistant HBV, controlling the quality of a microarray, determining the presence and ratio of more than one type, and determining positive and false positive probes at the same time using the microarray. The microarray, which includes the target probes for drug-resistant HBV detection, the QC probes, and the negative control probes, can detect a drug-resistant HBV, control quality in fabrication of microarrays and hybridization, determine the presence and ratio of more than one type, i.e., a wild type and a mutant, determine positive and false positive probes by measuring a background of non-specific cross-hybridization, and discriminate homozygotes and heterozygotes. When a plurality of sets of probes, each set containing target probes, QC probes, and negative control probes, are immobilized on a support of the microarray, detection of resistance in HBV to multiple drugs, quality control, and determination as to the presence and ratio of a wild type and a mutant and whether each probe is positive or false positive can be rapidly and accurately performed.

摘要翻译： 提供了使用用于耐药HBV检测的靶探针混合物制造的微阵列，用于控制探针杂交质量的控制探针和微阵列的制造以及用于确定多于一种类型的存在和比例的阴性对照探针， 密码子中的野生型和突变体，测量非特异性交叉杂交的背景，以及鉴别纯合子和杂合子，以及检测耐药性HBV的方法，控制微阵列的质量，确定存在和比例 的多种类型，并使用微阵列同时确定阳性和假阳性探针。 包括用于耐药HBV检测的目标探针，QC探针和阴性对照探针的微阵列可以检测耐药性HBV，控制微阵列和杂交的制备质量，确定多于一个的存在和比例 一种类型，即野生型和突变体，通过测量非特异性交叉杂交的背景，并鉴别纯合子和杂合子来确定阳性和假阳性探针。 当将包含目标探针，QC探针和阴性对照探针的多组探针固定在微阵列的载体上时，检测HBV对多种药物的耐药性，质量控制和确定存在和 野生型和突变体的比例以及每种探针是阳性还是假阳性可以快速和准确地进行。

公开(公告)号：US20090286691A1

公开(公告)日：2009-11-19

申请号：US12161691

申请日：2006-01-20

申请人： Cheol-Min Kim ,  Hee-Kyung Park ,  Hyun-Jung Jang ,  Eun-Sil Song

发明人： Cheol-Min Kim ,  Hee-Kyung Park ,  Hyun-Jung Jang ,  Eun-Sil Song

IPC分类号： C40B30/04 ,  C07H21/04 ,  C12Q1/68 ,  C40B40/06 ,  C40B40/08

CPC分类号： C12Q1/689

摘要： The present invention relates to oligonucleotides for detection of sepsis-causing bacteria and a detection method using the oligonucleotides, more particularly to a microarry comprising at least one of gram positive bacteria-specific and gram negative bacteria-specific oligonucleotides, sepsis-causing bacteria's genus-specific and species-specific oligonucleotides designed from the ITS target region which is hypervariable base sequence of sepsis-causing bacteria as probes, and a detection method and a diagnosis kit by using the same.According to the present invention, the present invention can provide an antibiotics therapy for accurately removing infectious agent related to sepsis by detecting existence of sepsis-causing bacteria and identifying gram positive- and gram negative-bacteria and genus and species of the bacteria, at once. And, the present invention can prevent a patient from abuse and misuse of antibiotics and decrease time of hospital treatment and medical cost of the patient. Further, the present invention has advantage of preventing complications and reducing mortality rate.

摘要翻译： 本发明涉及用于检测败血症细菌的寡核苷酸和使用寡核苷酸的检测方法，更具体地涉及包含革兰氏阳性细菌特异性和革兰氏阴性细菌特异性寡核苷酸中的至少一种的微阵列，败血症细菌的属 - 由作为败血症的细菌作为探针的高变碱基序列的ITS靶区设计的特异性和物种特异性寡核苷酸，以及使用该检测方法和诊断试剂盒。 根据本发明，本发明可以提供一种用于通过检测脓毒症引起的细菌的存在并一次鉴定革兰氏阳性和革兰氏阴性细菌以及细菌的种类和物种来精确地去除与败血症相关的传染原的抗生素治疗 。 并且，本发明可以防止患者滥用和滥用抗生素，并减少患者的医院治疗时间和医疗费用。 此外，本发明具有防止并发症和降低死亡率的优点。