发明申请
- 专利标题: Gene Detection Method, and Intercalator
- 专利标题(中): 基因检测方法和嵌入剂
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申请号: US11631609申请日: 2005-06-30
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公开(公告)号: US20090000962A1公开(公告)日: 2009-01-01
- 发明人: Junichi Hori , Ryusuke Murayama , Jinpei Tabata , Katsuhiko Bando , Naoyoshi Egashira
- 申请人: Junichi Hori , Ryusuke Murayama , Jinpei Tabata , Katsuhiko Bando , Naoyoshi Egashira
- 优先权: JP2004-199157 20040706
- 国际申请: PCT/JP05/12080 WO 20050630
- 主分类号: G01N27/26
- IPC分类号: G01N27/26 ; C07H21/00
摘要:
A gene detection method for detecting a gene having a specific sequence with high sensitivity is provided. The gene detection method comprises a double-stranded nucleic acid formation step of forming double-stranded nucleic acid by hybridizing a nucleic acid probe immobilized on an electrode and a gene sample; a step of adding an intercalator which comprises a compound having a double-stranded nucleic acid binding site Ia that is specifically intercalated into the double-stranded nucleic acid and is covalently bonded to the double-stranded nucleic acid by light irradiation, an electrochemical active site Fa having electrochemical activity, and a connecting site La for connecting the double-stranded nucleic acid binding site Ia and the electrochemical active site Fa, and has an substitution group comprising one of compounds represented by -Lb-Ib and -Lc-Fb or a combination of the both compounds, at each of the respective sites; a light irradiation step of covalently bonding the double-stranded nucleic acid and the intercalator; and a detection step of detecting the intercalator covalently bonded to the double-stranded nucleic acid by electrochemical measurement.
公开/授权文献
- US07833406B2 Gene detection method, and intercalator 公开/授权日:2010-11-16
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