Invention Application
US20090017490A1 DNA Fragment Method for Producing Transformant for Protein Production and Utilization Thereof
有权
用于产生蛋白质生产和利用的转化体的DNA片段方法
- Patent Title: DNA Fragment Method for Producing Transformant for Protein Production and Utilization Thereof
- Patent Title (中): 用于产生蛋白质生产和利用的转化体的DNA片段方法
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Application No.: US10574112Application Date: 2004-10-01
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Publication No.: US20090017490A1Publication Date: 2009-01-15
- Inventor: Masashi Mori , Koji Dohi , Masayuki Ishikawa , Tetsuo Meshi , Masaki Nishikiori , Atsushi Tamai
- Applicant: Masashi Mori , Koji Dohi , Masayuki Ishikawa , Tetsuo Meshi , Masaki Nishikiori , Atsushi Tamai
- Priority: JP2003-343747 20031001; JP2003-350091 20031008; JP2004-056912 20040301
- International Application: PCT/JP04/14487 WO 20041001
- Main IPC: C12Q1/04
- IPC: C12Q1/04 ; C07H21/04 ; C12N15/63 ; C12P21/00 ; C12N5/10
Abstract:
An expression vector is constructed by transferring recombinant tomato mosaic virus (ToMV) cDNA, in which a coat protein gene of ToMV having a suppressor against a virus resistant reaction has been substituted by a GFP gene, into the downstream of a promoter capable of inducing steroid hormone-dependent transcription. In a transformed tobacco BY-2 cell obtained by transferring the above expression vector into a tobacco BY-2 cells, steroid hormone-dependent transcription is induced, thereby enabling the amplification of mRNA of the GFP gene and induction of the expression of GFP.
Public/Granted literature
- US08507220B2 Tobacco BY2 cells containing a tomato mosaic virus vector for protein production Public/Granted day:2013-08-13
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