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    Tobacco BY2 cells containing a tomato mosaic virus vector for protein production
    1.
    发明授权
    Tobacco BY2 cells containing a tomato mosaic virus vector for protein production 有权
    含有番茄花叶病毒载体的烟草BY2细胞用于蛋白质生产

    公开(公告)号：US08507220B2

    公开(公告)日：2013-08-13

    申请号：US10574112

    申请日：2004-10-01

    申请人： Masashi Mori ,  Koji Dohi ,  Masayuki Ishikawa ,  Tetsuo Meshi ,  Masaki Nishikiori ,  Atsushi Tamai

    发明人： Masashi Mori ,  Koji Dohi ,  Masayuki Ishikawa ,  Tetsuo Meshi ,  Masaki Nishikiori ,  Atsushi Tamai

    IPC分类号： C12P21/02 ,  C12N15/04 ,  C12N15/83

    CPC分类号： C12N15/8237

    摘要： An expression vector is constructed by transferring recombinant tomato mosaic virus (ToMV) cDNA, in which a coat protein gene of ToMV having a suppressor against a virus resistant reaction has been substituted by a GFP gene, into the downstream of a promoter capable of inducing steroid hormone-dependent transcription. In a transformed tobacco BY-2 cell obtained by transferring the above expression vector into a tobacco BY-2 cells, steroid hormone-dependent transcription is induced, thereby enabling the amplification of mRNA of the GFP gene and induction of the expression of GFP.

    摘要翻译： 通过转移重组番茄花叶病毒（ToMV）cDNA构建表达载体，其中具有抗病毒抗性反应的抑制剂的ToMV的外壳蛋白基因已被GFP基因取代成能够诱导类固醇的启动子的下游 激素依赖性转录。 在通过将上述表达载体转移到烟草BY-2细胞获得的转化烟草BY-2细胞中，诱导类固醇激素依赖性转录，从而能够扩增GFP基因的mRNA并诱导GFP表达。

    Virus vector and use thereof
    3.
    发明授权
    Virus vector and use thereof 有权
    病毒载体及其用途

    公开(公告)号：US08586826B2

    公开(公告)日：2013-11-19

    申请号：US12450999

    申请日：2008-04-11

    申请人： Masashi Mori ,  Koji Dohi

    发明人： Masashi Mori ,  Koji Dohi

    IPC分类号： C07K14/005 ,  C12N15/67 ,  C12N15/40

    CPC分类号： C12N15/8203 ,  C12N15/8257 ,  C12N2770/00043

    摘要： It is intended to provide a polynucleotide comprising a viral base sequence, the viral base sequence containing: a first base sequence encoding a viral replication protein, and a second base sequence encoding a viral movement protein, the second base sequence being located downstream of the first base sequence and having a linking site for linking with an exogenous base sequence encoding a polypeptide to be expressed, the linking site being located downstream of the second base sequence, the second base sequence being obtained by modifying with a base sequence in a native sequence derived from a virus by insertion, substitution, or addition. By using this, a vector containing a viral base sequence is constructed, and a protein is efficiently produced without worsening growth of a host cell containing the vector.

    摘要翻译： 旨在提供包含病毒碱基序列的多核苷酸，所述病毒碱基序列包含编​​码病毒复制蛋白的第一碱基序列和编码病毒运动蛋白的第二碱基序列，所述第二碱基序列位于第一 碱基序列并具有与编码待表达多肽的外源碱基序列连接的连接位点，所述连接位点位于第二碱基序列的下游，第二碱基序列通过用天然序列衍生的碱基序列修饰获得 从病毒通过插入，替换或添加。 通过使用该方法构建含有病毒碱基序列的载体，能有效地产生蛋白质，而不会使包含载体的宿主细胞的生长恶化。

    VIRUS VECTOR AND USE THEREOF
    4.
    发明申请
    VIRUS VECTOR AND USE THEREOF 有权
    病毒矢量及其使用

    公开(公告)号：US20110107466A1

    公开(公告)日：2011-05-05

    申请号：US12450999

    申请日：2008-04-11

    申请人： Masashi Mori ,  Koji Dohi

    发明人： Masashi Mori ,  Koji Dohi

    IPC分类号： C07H21/04 ,  C12N15/63 ,  A01H5/00 ,  C12N5/10 ,  C12P21/00

    CPC分类号： C12N15/8203 ,  C12N15/8257 ,  C12N2770/00043

    摘要： It is intended to provide a polynucleotide comprising a viral base sequence, the viral base sequence containing: a first base sequence encoding a viral replication protein, and a second base sequence encoding a viral movement protein, the second base sequence being located downstream of the first base sequence and having a linking site for linking with an exogenous base sequence encoding a polypeptide to be expressed, the linking site being located downstream of the second base sequence, the second base sequence being obtained by modifying with a base sequence in a native sequence derived from a virus by insertion, substitution, or addition. By using this, a vector containing a viral base sequence is constructed, and a protein is efficiently produced without worsening growth of a host cell containing the vector.

    摘要翻译： 旨在提供包含病毒碱基序列的多核苷酸，所述病毒碱基序列包含编​​码病毒复制蛋白的第一碱基序列和编码病毒运动蛋白的第二碱基序列，所述第二碱基序列位于第一 碱基序列并具有与编码待表达多肽的外源碱基序列连接的连接位点，所述连接位点位于第二碱基序列的下游，第二碱基序列通过用天然序列衍生的碱基序列修饰获得 从病毒通过插入，替换或添加。 通过使用该方法构建含有病毒碱基序列的载体，能有效地产生蛋白质，而不会使包含载体的宿主细胞的生长恶化。

    Wheel position detecting device that performs dedicated local communication for each wheel and tire air pressure detecting device including the same
    7.
    发明授权
    Wheel position detecting device that performs dedicated local communication for each wheel and tire air pressure detecting device including the same 有权
    车轮位置检测装置，对包括该车轮的轮胎和轮胎气压检测装置进行专用的局部通信

    公开(公告)号：US07705714B2

    公开(公告)日：2010-04-27

    申请号：US11902070

    申请日：2007-09-18

    申请人： Masashi Mori

    发明人： Masashi Mori

    IPC分类号： B60Q1/00

    CPC分类号： B60C23/0416

    摘要： A wheel position detecting device and a tire air pressure detecting device including the wheel position detecting device are provided. The wheel position detecting device includes triggering devices and a receiver mounted on the vehicle-body side, and transmitters mounted on the wheels. Each triggering device outputs a trigger signal to each transmitter. In response to the trigger signal, each transmitter transmits a frame to the receiver. The receiver receives the frame and judges whether the transmitter is mounted on a subject wheel, based on whether reception strength indicated by reception strength data stored in the frame is within a range set in advance.

    摘要翻译： 提供了一种车轮位置检测装置和包括车轮位置检测装置的轮胎气压检测装置。 车轮位置检测装置包括触发装置和安装在车体侧的接收器以及安装在车轮上的发射器。 每个触发装置向每个发射器输出触发信号。 响应于触发信号，每个发射机向接收机发射一帧。 接收机接收帧，并基于由帧中存储的接收强度数据指示的接收强度是否在预先设定的范围内，判断发射机是否安装在被摄对象轮上。

    TRIGGER UNIT MOUNTING STRUCTURE FOR WHEEL POSITION DETECTION SYSTEM
    8.
    发明申请
    TRIGGER UNIT MOUNTING STRUCTURE FOR WHEEL POSITION DETECTION SYSTEM 失效
    用于车轮位置检测系统的触发单元安装结构

    公开(公告)号：US20090145217A1

    公开(公告)日：2009-06-11

    申请号：US12330791

    申请日：2008-12-09

    申请人： Takashi Saitou ,  Akira Takaoka ,  Nobuya Watabe ,  Masashi Mori

    发明人： Takashi Saitou ,  Akira Takaoka ,  Nobuya Watabe ,  Masashi Mori

    IPC分类号： B60C23/04

    CPC分类号： B60C23/0416 ,  B60C23/0444 ,  B60C23/045

    摘要： A first trigger unit and a second trigger unit output trigger signals to transceivers disposed for the four wheels of a vehicle. The first and second trigger units are attached to wall surfaces of tire housings corresponding to the left front and left rear wheels respectively. Each of the first and second trigger units is arranged in a position of the wall surface having an angle from 5 to 25 degrees with respect to the side of the corresponding wheel. Transceivers attached to a right front and right rear wheels are thus located farther from the trigger units than the left front and left rear wheels, respectively. Such arrangement allows the transceivers attached to the right front and right rear wheels to receive the outputted trigger signals with sufficient accuracy due to high field strength.

    摘要翻译： 第一触发单元和第二触发单元向车辆的四个车轮设置的收发器输出触发信号。 第一和第二触发单元分别附接到对应于左前后轮和左后轮的轮胎壳体的壁表面。 第一和第二触发单元中的每一个被布置在壁表面相对于相应的轮的侧面具有5至25度的角度的位置。 因此，附接到右前轮和右后轮的收发器分别比左前轮和左后轮远离扳机单元。 这样的布置允许附接到右前轮和右后轮的收发器由于高场强而以足够的精度接收输出的触发信号。

    Apparatus for detecting positions of wheels and method of manufacturing the same
    9.
    发明申请
    Apparatus for detecting positions of wheels and method of manufacturing the same 有权
    用于检测车轮位置的装置及其制造方法

    公开(公告)号：US20080055063A1

    公开(公告)日：2008-03-06

    申请号：US11896741

    申请日：2007-09-05

    申请人： Masashi Mori

    发明人： Masashi Mori

    IPC分类号： B60C23/02

    CPC分类号： B60C23/0416

    摘要： A wheel position detecting apparatus comprises transmitters, a triggering device, and a receiver. The transmitters are attached to the plurality of wheels respectively. Each transmitter transmits a frame responsively to a triggering signal from the triggering device. The frame includes data indicating a reception intensity of the triggering signal. The triggering device is disposed in a vehicle body and outputs the triggering signal toward the transmitters attached to the plurality of wheels. This triggering device is poisoned nearest to a specified wheel among the plurality of wheels to which the triggering signal is outputted, the specified wheel being influenced most heavily by noise generated in the vehicle. The receiver, disposed to the vehicle body, receives the frame and uses the data indicating the reception intensity of the triggering signal to detect positions of the wheels by determining which transmitter is attached to which wheel.

    摘要翻译： 轮位置检测装置包括发射器，触发装置和接收器。 发射机分别连接到多个车轮。 每个发射机响应于来自触发装置的触发信号发送帧。 帧包括指示触发信号的接收强度的数据。 触发装置设置在车身中并将触发信号输出到附接到多个车轮的发射器。 该触发装置在触发信号被输出的多个车轮中最接近特定车轮时中毒，指定的车轮受到车辆中产生的噪声的影响最大。 设置在车身上的接收器接收帧并使用指示触发信号的接收强度的数据来通过确定哪个发射器附接到哪个车轮来检测车轮的位置。

    Tire location detecting apparatus with transmitters configured to transmit signals at predetermined angular positions
    10.
    发明申请
    Tire location detecting apparatus with transmitters configured to transmit signals at predetermined angular positions 有权
    具有发射机的轮胎位置检测装置被配置为在预定角度位置发射信号

    公开(公告)号：US20060250228A1

    公开(公告)日：2006-11-09

    申请号：US11429441

    申请日：2006-05-08

    申请人： Masashi Mori ,  Nobuya Watabe

    发明人： Masashi Mori ,  Nobuya Watabe

    IPC分类号： B60C23/00

    CPC分类号： B60C23/0416 ,  B60C23/0488

    摘要： According to the invention, a wheel identifying apparatus includes a plurality of transmitters and a receiver. Each of the transmitters is mounted on one of wheels of a vehicle with a given distance from a rotation axis of the wheel. Each of the transmitters is configured to transmit a signal when an angular position thereof coincides with a predetermined angular position. The receiver includes a receiving antenna located at such a predetermined position on a body of the vehicle that all distances from the receiving antenna to the rotation axes of the wheels of the vehicle are different from each other. The receiver works to receive all the signals transmitted from the transmitters, detect the received signal levels of the signals, and identify, for each of the signals, the wheel on which the transmitter having transmitted the signal is located, based on the detected received signal level of the signal.

    摘要翻译： 根据本发明，车轮识别装置包括多个发射机和接收机。 每个发射器安装在车轮的一个车轮上，距离车轮的旋转轴线有一定的距离。 每个发射器被配置成当其角度位置与预定角位置一致时发射信号。 接收机包括位于车辆本体上的这种预定位置处的接收天线，所述距离从接收天线到车辆的车轮的旋转轴线的所有距离彼此不同。 接收机工作以接收从发射机发送的所有信号，检测信号的接收信号电平，并且基于检测到的接收信号，识别发送信号的发射机所在的车轮上的每个信号 信号电平。