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发明申请
US20090023173A1 Luminescence-based methods and probes for measuring cytochrome P450 activity 有权
用于测量细胞色素P450活性的基于发光的方法和探针

Luminescence-based methods and probes for measuring cytochrome P450 activity
摘要：
The invention provides compounds, compositions, methods, substrates, and kits useful for analyzing the metabolic activity in cells, tissue, and animals and for screening test compounds for their effect on cytochrome P450 activity. In particular, a one-step and two-step methods using luminogenic molecules, e.g. luciferins or coelenterazines, that are cytochrome P450 substrates and that are also bioluminescent enzyme, e.g., luciferase, pro-substrates are provided. The present method further provides a method for stabilizing and prolonging the luminescent signal in a luciferase-based assay using luciferase stabilizing agents such as reversible luciferase inhibitors.
摘要（中）：

本发明提供了可用于分析细胞，组织和动物中的代谢活性的化合物，组合物，方法，底物和试剂盒，并用于筛选测试化合物对细胞色素P450活性的影响。 特别地，使用发光分子的一步和两步法。 荧光素或coelenterazine，即细胞色素P450底物，并且也是生物发光酶，例如荧光素酶，原底物。 本发明方法还提供了一种使用荧光素酶稳定剂如可逆荧光素酶抑制剂在基于荧光素酶的测定中稳定和延长发光信号的方法。

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