发明申请
- 专利标题： Preparation of templates for nucleic acid sequencing
- 专利标题（中）： 核酸测序模板的制备
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申请号： US11989169申请日： 2006-07-20
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公开(公告)号： US20090118128A1公开(公告)日： 2009-05-07
- 发明人: Xiaohai Liu , John Milton , Geoffrey Paul Smith , Colin Barnes , Isabelle Marie Julia Rasolonjatovo , Roberto Rigatti , Xiaolin Wu , Tobias William Barr Ost , Graham John Worsley , David James Earnshaw , Gerardo Turcatti , Anthony Romieu
- 申请人： Xiaohai Liu , John Milton , Geoffrey Paul Smith , Colin Barnes , Isabelle Marie Julia Rasolonjatovo , Roberto Rigatti , Xiaolin Wu , Tobias William Barr Ost , Graham John Worsley , David James Earnshaw , Gerardo Turcatti , Anthony Romieu
- 优先权： GB0514936.4 20050720
- 国际申请： PCT/GB2006/002687 WO 20060720
- 主分类号： C40B20/00
- IPC分类号： C40B20/00 ; C07H1/00 ; C12P19/34 ; C40B50/06
摘要：
The invention relates to methods of generating templates for a nucleic acid sequencing reaction which comprise: providing at least one double-stranded nucleic acid molecule, wherein both strands of the double-stranded nucleic acid molecule are attached to a solid support at the 5′ end, cleaving one or both strands of the double-stranded nucleic acid molecule, and subjecting the cleaved strand(s) to denaturing conditions to remove the portion of the cleaved strand(s) not attached to the solid support, thereby generating a partially or substantially single-stranded template for a nucleic acid sequencing reaction.
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