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1.发明授权公开(公告)号：US08460910B2
公开(公告)日：2013-06-11
申请号：US10571706
申请日：2004-09-10
申请人： Geoffrey Paul Smith , David Mark Dunstan Bailey , Raquel Maria Sanches , Harold Swerdlow , David James Earnshaw
发明人： Geoffrey Paul Smith , David Mark Dunstan Bailey , Raquel Maria Sanches , Harold Swerdlow , David James Earnshaw
CPC分类号： C12N9/1252 , C12Q1/6869 , C12Y207/07007
摘要： The invention relates to modified polymerase enzymes which exhibit improved incorporation of nucleotide analogues bearing substituents at the 3′ position of the sugar moiety that are larger in size than the naturally occurring 3′ hydroxyl group. Also described are methods of using the polymerases to incorporate nucleotides into polynucleotides, particularly in the context of DNA sequencing.
摘要翻译： 本发明涉及修饰的聚合酶，其表现出在糖部分的3'位置具有比天然存在的3'羟基更大尺寸的具有取代基的核苷酸类似物的改进掺入。 还描述了使用聚合酶将核苷酸掺入多核苷酸的方法，特别是在DNA测序的上下文中。
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公开(公告)号：US09328378B2
公开(公告)日：2016-05-03
申请号：US12309772
申请日：2007-07-30
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6855 , C12Q1/6806 , C12Q1/6874 , C12Q2533/107 , C12Q2525/101
摘要： The invention relates to a method of preparing a library of template polynucleotides which reduces and/or prevents the formation of adaptor-dimers. The invention also relates to the use of a library of templates prepared using the method of the invention for solid-phase nucleic acid amplification. In particular, the invention relates to a method of preparing a library of template polynucleotides which have common sequences at their 5′ ends and at their 3′ ends which is substantially free of adaptor-dimers.
摘要翻译： 本发明涉及一种制备模板多核苷酸文库的方法，其减少和/或防止适配剂二聚体的形成。 本发明还涉及使用本发明的方法制备的模板文库用于固相核酸扩增的用途。 特别地，本发明涉及一种制备模板多核苷酸文库的方法，该文库在其5'末端和3'末端具有基本上不含适配剂二聚体的共同序列。
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公开(公告)号：US20090118128A1
公开(公告)日：2009-05-07
申请号：US11989169
申请日：2006-07-20
申请人： Xiaohai Liu , John Milton , Geoffrey Paul Smith , Colin Barnes , Isabelle Marie Julia Rasolonjatovo , Roberto Rigatti , Xiaolin Wu , Tobias William Barr Ost , Graham John Worsley , David James Earnshaw , Gerardo Turcatti , Anthony Romieu
发明人： Xiaohai Liu , John Milton , Geoffrey Paul Smith , Colin Barnes , Isabelle Marie Julia Rasolonjatovo , Roberto Rigatti , Xiaolin Wu , Tobias William Barr Ost , Graham John Worsley , David James Earnshaw , Gerardo Turcatti , Anthony Romieu
CPC分类号： B01J19/0046 , B01J2219/00596 , B01J2219/00608 , B01J2219/00675 , B01J2219/00722 , C12Q1/6806 , C12Q1/6834 , C12Q1/6874 , C12Q2565/543 , C12Q2535/101 , C12Q2523/113 , C12Q2523/107 , C12Q2521/331 , C12Q2521/307
摘要： The invention relates to methods of generating templates for a nucleic acid sequencing reaction which comprise: providing at least one double-stranded nucleic acid molecule, wherein both strands of the double-stranded nucleic acid molecule are attached to a solid support at the 5′ end, cleaving one or both strands of the double-stranded nucleic acid molecule, and subjecting the cleaved strand(s) to denaturing conditions to remove the portion of the cleaved strand(s) not attached to the solid support, thereby generating a partially or substantially single-stranded template for a nucleic acid sequencing reaction.
摘要翻译： 本发明涉及产生用于核酸测序反应的模板的方法，其包括：提供至少一个双链核酸分子，其中双链核酸分子的两条链在5'末端连接到固体支持物上 切割双链核酸分子的一条或两条链，并使切割的链进行变性条件以除去未连接到固体支持物上的部分切割的链，从而产生部分或基本上 用于核酸测序反应的单链模板。
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公开(公告)号：US09085802B2
公开(公告)日：2015-07-21
申请号：US14166670
申请日：2014-01-28
申请人： Xiaohai Liu , John Milton , Geoffrey Paul Smith , Colin Lloyd Barnes , Isabelle Marie Julia Rasolonjatovo , Roberto Rigatti , Xiaolin Wu , Tobias William Barr Ost , Graham John Worsley , David James Earnshaw , Gerardo Turcatti , Anthony Romieu
发明人： Xiaohai Liu , John Milton , Geoffrey Paul Smith , Colin Lloyd Barnes , Isabelle Marie Julia Rasolonjatovo , Roberto Rigatti , Xiaolin Wu , Tobias William Barr Ost , Graham John Worsley , David James Earnshaw , Gerardo Turcatti , Anthony Romieu
IPC分类号： C12Q1/68
CPC分类号： B01J19/0046 , B01J2219/00596 , B01J2219/00608 , B01J2219/00675 , B01J2219/00722 , C12Q1/6806 , C12Q1/6834 , C12Q1/6874 , C12Q2565/543 , C12Q2535/101 , C12Q2523/113 , C12Q2523/107 , C12Q2521/331 , C12Q2521/307
摘要： The invention relates to methods of generating templates for a nucleic acid sequencing reaction which comprise: providing at least one double-stranded nucleic acid molecule, wherein both strands of the double-stranded nucleic acid molecule are attached to a solid support at the 5′ end, cleaving one or both strands of the double-stranded nucleic acid molecule, and subjecting the cleaved strand(s) to denaturing conditions to remove the portion of the cleaved strand(s) not attached to the solid support, thereby generating a partially or substantially single-stranded template for a nucleic acid sequencing reaction.
摘要翻译： 本发明涉及产生用于核酸测序反应的模板的方法，其包括：提供至少一个双链核酸分子，其中双链核酸分子的两条链在5'末端连接到固体支持物 切割双链核酸分子的一条或两条链，并使切割的链进行变性条件以除去未连接到固体支持物上的部分切割的链，从而产生部分或基本上 用于核酸测序反应的单链模板。
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公开(公告)号：US08715966B2
公开(公告)日：2014-05-06
申请号：US13450920
申请日：2012-04-19
申请人： Liu Xiaohai , John Milton , Geoffrey Paul Smith , Colin Lloyd Barnes , Isabelle Marie Julia Rasolonjatovo , Roberto Rigatti , Xiaolin Wu , Tobias William Barr Ost , Graham John Worsley , David James Earnshaw , Gerardo Turcatti , Anthony Romieu
发明人： Liu Xiaohai , John Milton , Geoffrey Paul Smith , Colin Lloyd Barnes , Isabelle Marie Julia Rasolonjatovo , Roberto Rigatti , Xiaolin Wu , Tobias William Barr Ost , Graham John Worsley , David James Earnshaw , Gerardo Turcatti , Anthony Romieu
CPC分类号： B01J19/0046 , B01J2219/00596 , B01J2219/00608 , B01J2219/00675 , B01J2219/00722 , C12Q1/6806 , C12Q1/6834 , C12Q1/6874 , C12Q2565/543 , C12Q2535/101 , C12Q2523/113 , C12Q2523/107 , C12Q2521/331 , C12Q2521/307
摘要： The invention relates to methods of generating templates for a nucleic acid sequencing reaction which comprise: providing at least one double-stranded nucleic acid molecule, wherein both strands of the double-stranded nucleic acid molecule are attached to a solid support at the 5′ end, cleaving one or both strands of the double-stranded nucleic acid molecule, and subjecting the cleaved strand(s) to denaturing conditions to remove the portion of the cleaved strand(s) not attached to the solid support, thereby generating a partially or substantially single-stranded template for a nucleic acid sequencing reaction.
摘要翻译： 本发明涉及产生用于核酸测序反应的模板的方法，其包括：提供至少一个双链核酸分子，其中双链核酸分子的两条链在5'末端连接到固体支持物上 切割双链核酸分子的一条或两条链，并使切割的链进行变性条件以除去未连接到固体支持物上的部分切割的链，从而产生部分或基本上 用于核酸测序反应的单链模板。
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公开(公告)号：US20120208194A1
公开(公告)日：2012-08-16
申请号：US13450920
申请日：2012-04-19
申请人： Xiaohai Liu , John Milton , Geoffrey Paul Smith , Colin Barnes , Isabelle Marie Julia Rasolonjatovo , Roberto Rigatti , Xiaolin Wu , Tobias William Barr Ost , Graham John Worsley , David James Earnshaw , Gerardo Turcatti , Anthony Romieu
发明人： Xiaohai Liu , John Milton , Geoffrey Paul Smith , Colin Barnes , Isabelle Marie Julia Rasolonjatovo , Roberto Rigatti , Xiaolin Wu , Tobias William Barr Ost , Graham John Worsley , David James Earnshaw , Gerardo Turcatti , Anthony Romieu
CPC分类号： B01J19/0046 , B01J2219/00596 , B01J2219/00608 , B01J2219/00675 , B01J2219/00722 , C12Q1/6806 , C12Q1/6834 , C12Q1/6874 , C12Q2565/543 , C12Q2535/101 , C12Q2523/113 , C12Q2523/107 , C12Q2521/331 , C12Q2521/307
摘要： The invention relates to methods of generating templates for a nucleic acid sequencing reaction which comprise: providing at least one double-stranded nucleic acid molecule, wherein both strands of the double-stranded nucleic acid molecule are attached to a solid support at the 5′ end, cleaving one or both strands of the double-stranded nucleic acid molecule, and subjecting the cleaved strand(s) to denaturing conditions to remove the portion of the cleaved strand(s) not attached to the solid support, thereby generating a partially or substantially single-stranded template for a nucleic acid sequencing reaction,
摘要翻译： 本发明涉及产生用于核酸测序反应的模板的方法，其包括：提供至少一个双链核酸分子，其中双链核酸分子的两条链在5'末端连接到固体支持物上 切割双链核酸分子的一条或两条链，并使切割的链进行变性条件以除去未连接到固体支持物上的部分切割的链，从而产生部分或基本上 用于核酸测序反应的单链模板，
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公开(公告)号：US20100167954A1
公开(公告)日：2010-07-01
申请号：US12309772
申请日：2007-07-30
CPC分类号： C12Q1/6855 , C12Q1/6806 , C12Q1/6874 , C12Q2533/107 , C12Q2525/101
摘要： The invention relates to a method of preparing a library of template polynucleotides which reduces and/or prevents the formation of adaptor-dimers. The invention also relates to the use of a library of templates prepared using the method of the invention for solid-phase nucleic acid amplification. In particular, the invention relates to a method of preparing a library of template polynucleotides which have common sequences at their 5′ ends and at their 3′ ends which is substantially free of adaptor-dimers.
摘要翻译： 本发明涉及一种制备模板多核苷酸文库的方法，其减少和/或防止适配剂二聚体的形成。 本发明还涉及使用本发明的方法制备的模板文库用于固相核酸扩增的用途。 特别地，本发明涉及一种制备模板多核苷酸文库的方法，该文库在其5'末端和3'末端具有基本上不含适配剂二聚体的共同序列。
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公开(公告)号：US20140349891A1
公开(公告)日：2014-11-27
申请号：US14166670
申请日：2014-01-28
申请人： Xiaohai Liu , John Milton , Geoffrey Paul Smith , Colin Lloyd Barnes , Isabelle Marie Julia Rasolonjatovo , Roberto Rigatti , Xiaolin Wu , Tobias William Barr Ost , Graham John Worsley , David James Earnshaw , Gerardo Turcatti , Anthony Romieu
发明人： Xiaohai Liu , John Milton , Geoffrey Paul Smith , Colin Lloyd Barnes , Isabelle Marie Julia Rasolonjatovo , Roberto Rigatti , Xiaolin Wu , Tobias William Barr Ost , Graham John Worsley , David James Earnshaw , Gerardo Turcatti , Anthony Romieu
IPC分类号： C12Q1/68
CPC分类号： B01J19/0046 , B01J2219/00596 , B01J2219/00608 , B01J2219/00675 , B01J2219/00722 , C12Q1/6806 , C12Q1/6834 , C12Q1/6874 , C12Q2565/543 , C12Q2535/101 , C12Q2523/113 , C12Q2523/107 , C12Q2521/331 , C12Q2521/307
摘要： The invention relates to methods of generating templates for a nucleic acid sequencing reaction which comprise: providing at least one double-stranded nucleic acid molecule, wherein both strands of the double-stranded nucleic acid molecule are attached to a solid support at the 5′ end, cleaving one or both strands of the double-stranded nucleic acid molecule, and subjecting the cleaved strand(s) to denaturing conditions to remove the portion of the cleaved strand(s) not attached to the solid support, thereby generating a partially or substantially single-stranded template for a nucleic acid sequencing reaction,
摘要翻译： 本发明涉及产生用于核酸测序反应的模板的方法，其包括：提供至少一个双链核酸分子，其中双链核酸分子的两条链在5'末端连接到固体支持物上 切割双链核酸分子的一条或两条链，并使切割的链进行变性条件以除去未连接到固体支持物上的部分切割的链，从而产生部分或基本上 用于核酸测序反应的单链模板，
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公开(公告)号：US20090117621A1
公开(公告)日：2009-05-07
申请号：US11989171
申请日：2006-07-20
申请人： Jonathan Mark Boutell , Geoffrey Paul Smith , Anthony James Cox , David James Earnshaw , Gary Paul Schroth
发明人： Jonathan Mark Boutell , Geoffrey Paul Smith , Anthony James Cox , David James Earnshaw , Gary Paul Schroth
CPC分类号： C12Q1/686 , B01J2219/00626 , B01J2219/00637 , B01J2219/00641 , B01J2219/00659 , B01J2219/00722 , C12Q2565/501
摘要： The invention relates to a method of amplifying one or more nucleic acid templates on a solid support in a nucleic acid amplification reaction, for example by solid-phase PCR using one or more amplification primers attached to the solid support. The method is characterised in that the amplification primers used comprise a template-specific portion which is a sequence of at least 26 consecutive nucleotides and are not capable of annealing to target regions in the template under conditions of the amplification reaction. The method is particularly useful for amplifying human genomic DNA.
摘要翻译： 本发明涉及在核酸扩增反应中扩增固体支持物上的一种或多种核酸模板的方法，例如通过使用连接至固体支持物的一种或多种扩增引物的固相PCR。 该方法的特征在于使用的扩增引物包含模板特异性部分，其是至少26个连续核苷酸的序列，并且在扩增反应的条件下不能退火到模板中的靶区域。 该方法对于扩增人类基因组DNA特别有用。
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