A vibration type microinjection device capable of ensuring smooth piercing of membranes having different properties such as a zona pellucida, a cell membrane and a nuclear membrane included in a fertilized egg with high accuracy and efficiency is provided.A vibration type microinjection device comprises a vibrator (28) which is connected in series with a micropipette (8) and which has a piezoelectric actuator (29) installed in a housing, and a signal control device (21) for controlling an electric signal applied to the piezoelectric actuator (29), wherein vibration is applied in the longitudinal direction of the micropipette (8) via the vibrator (28) by inputting an electric signal to the piezoelectric actuator (29). By such configuration, smooth piercing of membranes having different properties such as a zona pellucida, a cell membrane and nuclear membrane included in a fertilized egg is realized with high accuracy and efficiency.