发明申请
- 专利标题: Microorganism that Displays Biotin on Cell Surface
- 专利标题(中): 在细胞表面显示生物素的微生物
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申请号: US12431102申请日: 2009-04-28
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公开(公告)号: US20090275110A1公开(公告)日: 2009-11-05
- 发明人: Akihiko Kondo , Hideki Fukuda , Tsutomu Tanaka , Hideo Noda
- 申请人: Akihiko Kondo , Hideki Fukuda , Tsutomu Tanaka , Hideo Noda
- 申请人地址: JP Amagasaki-shi JP Amagasaki-shi
- 专利权人: BIO-ENERGY CORPORATION,KANSAI CHEMICAL ENGINEERING CO., LTD.
- 当前专利权人: BIO-ENERGY CORPORATION,KANSAI CHEMICAL ENGINEERING CO., LTD.
- 当前专利权人地址: JP Amagasaki-shi JP Amagasaki-shi
- 优先权: JP2008-118136 20080430
- 主分类号: C12N1/20
- IPC分类号: C12N1/20 ; C12N1/16
摘要:
Provided is a microorganism that can display, on the cell surface, any molecules other than a molecule comprising amino acids, more specifically, a microorganism that displays biotin on a cell surface. The microorganism is capable of co-expressing a biotinylating enzyme and an acceptor peptide having a sequence recognized by the biotinylating enzyme, wherein the acceptor peptide is expressed on the cell surface, so that lysine of the acceptor peptide is biotinylated to display biotin on the cell surface. Also provided is a method for displaying an intended molecule, including not only a molecule comprising amino acids but also any molecules, on a cell surface of a microorganism.
公开/授权文献
- US08252577B2 Microorganism that displays biotin on cell surface 公开/授权日:2012-08-28
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