发明申请
- 专利标题: DNA POLYMERASES AND RELATED METHODS
- 专利标题(中): DNA聚合物及相关方法
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申请号: US12425303申请日: 2009-04-16
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公开(公告)号: US20090280539A1公开(公告)日: 2009-11-12
- 发明人: Keith A. Bauer , Ellen Fiss , David H. Gelfand , Edward S. Smith , Shawn Suko , Olga Budker , Nancy Schoenbrunner , Susanne Stoffel
- 申请人: Keith A. Bauer , Ellen Fiss , David H. Gelfand , Edward S. Smith , Shawn Suko , Olga Budker , Nancy Schoenbrunner , Susanne Stoffel
- 申请人地址: US CA Pleasanton
- 专利权人: Roche Molecular Systems, Inc.
- 当前专利权人: Roche Molecular Systems, Inc.
- 当前专利权人地址: US CA Pleasanton
- 主分类号: C12P19/34
- IPC分类号: C12P19/34 ; C12N9/12
摘要:
Disclosed are mutant DNA polymerases having improved extension rates relative to a corresponding, unmodified polymerase. The mutant polymerases are useful in a variety of disclosed primer extension methods. The mutant polymerases overcome the inhibitory effects by an intercalating dye. Therefore, the mutant polymerases are useful in a variety of disclosed methods in combination with an intercalating dye. Also disclosed are related compositions, including recombinant nucleic acids, vectors, and host cells, which are useful, e.g., for production of the mutant DNA polymerases.
公开/授权文献
- US09102924B2 DNA polymerases and related methods 公开/授权日:2015-08-11
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