发明申请
US20090306188A1 IMPORTATION OF MITOCHONDRIAL PROTEIN BY AN ENHANCED ALLOTOPIC APPROACH
有权
通过增强的同种异体方法进行麻醉蛋白质的输入
- 专利标题: IMPORTATION OF MITOCHONDRIAL PROTEIN BY AN ENHANCED ALLOTOPIC APPROACH
- 专利标题(中): 通过增强的同种异体方法进行麻醉蛋白质的输入
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申请号: US11913618申请日: 2006-05-03
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公开(公告)号: US20090306188A1公开(公告)日: 2009-12-10
- 发明人: Marisol Corral-Debrinski , José-Alain Sahel , Valérie Kaltimbacher , Crystel Bonnet
- 申请人: Marisol Corral-Debrinski , José-Alain Sahel , Valérie Kaltimbacher , Crystel Bonnet
- 申请人地址: FR Montreuil
- 专利权人: INSERM (INSTITUT NATIONAL DE AL SANTE ET DE AL REC
- 当前专利权人: INSERM (INSTITUT NATIONAL DE AL SANTE ET DE AL REC
- 当前专利权人地址: FR Montreuil
- 国际申请: PCT/EP06/05323 WO 20060503
- 主分类号: A61K31/7052
- IPC分类号: A61K31/7052 ; C12N15/63 ; C07H21/00 ; C12N5/00 ; A61P43/00
摘要:
The inventors demonstrate that mRNA sorting to the mitochondrial surface is an efficient way to proceed to such an allotopic expression, and that this mRNA sorting can be controlled by selecting appropriate mitochondrion-targeting sequence (MTS) and appropriate 3′UTR sequences. The CDS sequence which codes for the protein to be delivered into the mitochondrion is guided by these appropriate MTS and 3′UTR sequences from the nuclear compartment to the mitochondrion-bound polysomes (where the CDS is translated), and aids in an efficient translocation of a mature functional protein into the mitochondria. Appropriate MTS and 3′UTR sequences correspond to those of nuclearly-transcribed mitochondrially-targeted mRNAs. The inventors demonstrate that, to obtain a stable therapeutically-effective importation, both an appropriate MTS and an appropriate 3′UTR should preferably be used.