发明申请
- 专利标题: Grating-based sensor combining label-free binding detection and fluorescence amplification and readout system for sensor
- 专利标题(中): 光栅传感器结合无标签结合检测和荧光放大和传感器读出系统
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申请号: US12387391申请日: 2009-04-30
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公开(公告)号: US20100003743A1公开(公告)日: 2010-01-07
- 发明人: Stephen C. Schulz , Brian T. Cunningham , Lance G. Laing , Peter Y. Li , Brant Binder , Gangadhar Jogikalmath , Alex Borsody
- 申请人: Stephen C. Schulz , Brian T. Cunningham , Lance G. Laing , Peter Y. Li , Brant Binder , Gangadhar Jogikalmath , Alex Borsody
- 专利权人: SRU Biosystems, Inc.
- 当前专利权人: SRU Biosystems, Inc.
- 主分类号: C12M1/34
- IPC分类号: C12M1/34
摘要:
A grating-based sensor is disclosed that has a grating structure constructed and designed for both evanescent resonance (ER) fluorescence detection and label-free detection applications. Some embodiments are disclosed which are optimized for ER detection in an air mode, in which the sample is dry. Other embodiments are optimized for ER detection in liquid mode, in which the sample is suspended in liquid medium such as water. One and two-dimensional gratings are also disclosed, including gratings characterized by unit cells with central posts, central holes, and two-level, two-dimensional gratings. A readout system for such sensors is also disclosed. One embodiment includes a first light source optimized for collecting label-free detection data, a second light source optimized for collecting ER fluorescence amplification data, and at least one detector. In one embodiment, the detector is an imaging system and includes a CCD camera for collecting both ER and label-free data. In other embodiments, the at least one detector takes the form of a spectrometer for collection of label-free data and a photomultiplier for collecting ER data. In other embodiments, a single light source such as a tunable laser or broad band light source is used.
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