Invention Application
US20100167356A1 Homologous recombination-based DNA cloning methods and compositions
有权
基于同源重组的DNA克隆方法和组合物
- Patent Title: Homologous recombination-based DNA cloning methods and compositions
- Patent Title (中): 基于同源重组的DNA克隆方法和组合物
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Application No.: US12660885Application Date: 2010-03-05
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Publication No.: US20100167356A1Publication Date: 2010-07-01
- Inventor: Weiqiang Liu , Ping Yang , Tao Wang , Zhuying Wang , Wenzhu Chen , Fang Liang Zhang
- Applicant: Weiqiang Liu , Ping Yang , Tao Wang , Zhuying Wang , Wenzhu Chen , Fang Liang Zhang
- Applicant Address: US NJ Piscataway
- Assignee: GENSCRIPT HOLDINGS (Hong Kong) LIMITED
- Current Assignee: GENSCRIPT HOLDINGS (Hong Kong) LIMITED
- Current Assignee Address: US NJ Piscataway
- Main IPC: C12P19/34
- IPC: C12P19/34 ; C12N15/66
Abstract:
Methods and compositions for cloning a donor DNA molecule into an acceptor vector at a predetermined location are described. The methods are based on homologous recombination mediated by in vitro treatment of the donor DNA and the acceptor vector with an enzyme cocktail containing an exonuclease and a single-stranded DNA binding protein.
Public/Granted literature
- US08815600B2 Homologous recombination-based DNA cloning methods and compositions Public/Granted day:2014-08-26
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