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17 条记录 (耗时 0.025 秒)

    Rapid electrophoresis binding method and related kits and compositions
    5.
    发明授权
    Rapid electrophoresis binding method and related kits and compositions 有权
    快速电泳结合方法及相关试剂盒及组合物

    公开(公告)号：US08968541B2

    公开(公告)日：2015-03-03

    申请号：US12797011

    申请日：2010-06-09

    申请人： Zhuying Wang ,  Jinyu Yang ,  Fanli Meng ,  Bing Lai ,  Zhan Dai ,  Fang Liang Zhang

    发明人： Zhuying Wang ,  Jinyu Yang ,  Fanli Meng ,  Bing Lai ,  Zhan Dai ,  Fang Liang Zhang

    IPC分类号： B01D57/02 ,  G01N33/559 ,  G01N27/447

    CPC分类号： G01N27/44726

    摘要： An improved staining method is described for staining a biopolymer such as a peptide, a protein, an RNA, a DNA, an oligosaccharide or a complex containing a peptide, a protein, an RNA, a DNA, or an oligosaccharide in a matrix. The method includes the step of moving a staining reagent into the matrix using an electric force. The staining time can be dramatically reduced relative to conventional technologies. The improved staining method can particularly be used, for example, to stain proteins after gel separation. Other related methods and related kits are also described.

    摘要翻译： 描述了一种改进的染色方法，用于染色基质中生物聚合物如肽，蛋白质，RNA，DNA，寡糖或含有肽，蛋白质，RNA，DNA或寡糖的复合物。 该方法包括使用电力将染色剂移动到基质中的步骤。 相对于常规技术，染色时间可以显着降低。 改进的染色方法可以特别用于例如在凝胶分离后染色蛋白质。 还描述了其它相关方法和相关试剂盒。

    Method of sequence optimization for improved recombinant protein expression using a particle swarm optimization algorithm
    6.
    发明授权
    Method of sequence optimization for improved recombinant protein expression using a particle swarm optimization algorithm 有权
    使用粒子群优化算法改进重组蛋白表达的序列优化方法

    公开(公告)号：US08326547B2

    公开(公告)日：2012-12-04

    申请号：US12894401

    申请日：2010-09-30

    申请人： Xiaowu Liu ,  Yun He ,  Zhuying Wang ,  Chunjiao Wang ,  Zhibing Liu ,  Tianhui Xia ,  Luquan Wang ,  Fang Liang Zhang

    发明人： Xiaowu Liu ,  Yun He ,  Zhuying Wang ,  Chunjiao Wang ,  Zhibing Liu ,  Tianhui Xia ,  Luquan Wang ,  Fang Liang Zhang

    IPC分类号： G06F19/10 ,  G06F19/00

    CPC分类号： G06F19/22 ,  G06F19/20

    摘要： An improved gene sequence optimization method, the systematic optimization method, is described for boosting the recombinant expression of genes in bacteria, yeast, insect and mammalian cells. This general method takes into account of multiple, preferably most or all, of the parameters and factors affecting protein expression including codon usage, tRNA usage, GC-content, ribosome binding sequences, promoter, 5′-UTR, ORF and 3′-UTR sequences of the genes to improve and optimize the gene sequences to boost the protein expression of the genes in bacteria, yeast, insect and mammalian cells. In particular, the invention relates to a system and a method for sequence optimization for improved recombinant protein expression using a particle swarm optimization algorithm. The improved systematic optimization method can be incorporated into a software for more efficient optimization.

    摘要翻译： 描述了改进的基因序列优化方法，该系统优化方法用于增强细菌，酵母，昆虫和哺乳动物细胞中基因的重组表达。 这种一般方法考虑了影响蛋白质表达的参数和因子的多个，优选大多数或全部，包括密码子使用，tRNA使用，GC含量，核糖体结合序列，启动子，5'-UTR，ORF和3'-UTR 改进和优化基因序列以增强细菌，酵母，昆虫和哺乳动物细胞中基因的蛋白质表达的基因序列。 特别地，本发明涉及使用粒子群优化算法改进重组蛋白表达的序列优化的系统和方法。 改进的系统优化方法可以被并入软件中以进行更有效的优化。

    Composition Related to Rapid ELISA Process
    7.
    发明申请
    Composition Related to Rapid ELISA Process 有权
    与快速ELISA方法相关的组成

    公开(公告)号：US20110008807A1

    公开(公告)日：2011-01-13

    申请号：US12885837

    申请日：2010-09-20

    申请人： Zhuying Wang ,  Fang Liang Zhang ,  Tao Bai

    发明人： Zhuying Wang ,  Fang Liang Zhang ,  Tao Bai

    IPC分类号： G01N33/543 ,  G01N33/566

    CPC分类号： G01N33/54393

    摘要： The present invention provides improved and rapid detection methods for an antigen such as a chemical compound, a peptide, a nucleic acid, or a protein released from cells or virus particles in situ. The detection time for an antigen can be dramatically reduced relative to conventional technologies. The technology can particularly be used, for example, to modify and reduce the detection time significantly in traditional ELISA, and also Western blot or Dot blot assays. The improved ELISA method is rapid, economical, reproducible, simple and automatable. Also provided are compositions and kits for using the improved ELISA methods for the rapid detection of antigens.

    摘要翻译： 本发明提供了一种抗原例如化合物，肽，核酸或原位从细胞或病毒颗粒释放的蛋白质的改进和快速检测方法。 相对于常规技术，抗原的检测时间可以显着降低。 该技术可以特别用于例如在传统ELISA中显着修饰和减少检测时间，以及Western印迹或斑点印迹测定。 改进的ELISA方法快速，经济，可重复，简单，可自动化。 还提供了使用改进的ELISA方法快速检测抗原的组合物和试剂盒。

    Rapid C-ELISA process and related compositions
    8.
    发明授权
    Rapid C-ELISA process and related compositions 有权
    快速C-ELISA方法及相关成分

    公开(公告)号：US07816092B2

    公开(公告)日：2010-10-19

    申请号：US12626327

    申请日：2009-11-25

    申请人： Zhuying Wang ,  Fang Liang Zhang ,  Tao Bai

    发明人： Zhuying Wang ,  Fang Liang Zhang ,  Tao Bai

    IPC分类号： G01N33/53

    CPC分类号： G01N33/54393

    摘要： The present invention provides improved and rapid detection methods for an antigen such as a chemical compound, a peptide, a nucleic acid, or a protein released from cells or virus particles in situ. The detection time for an antigen can be dramatically reduced relative to conventional technologies. The technology can particularly be used, for example, to modify and reduce the detection time significantly in traditional ELISA, and also Western blot or Dot blot assays. The improved ELISA method is rapid, economical, reproducible, simple and automatable. Also provided are compositions and kits for using the improved ELISA methods for the rapid detection of antigens.

    摘要翻译： 本发明提供了一种抗原例如化合物，肽，核酸或原位从细胞或病毒颗粒释放的蛋白质的改进和快速检测方法。 相对于常规技术，抗原的检测时间可以显着降低。 该技术可以特别用于例如在传统ELISA中显着修饰和减少检测时间，以及Western印迹或斑点印迹测定。 改进的ELISA方法快速，经济，可重复，简单，可自动化。 还提供了使用改进的ELISA方法快速检测抗原的组合物和试剂盒。

    RAPID ELISA PROCESSES AND RELATED COMPOSITIONS
    9.
    发明申请
    RAPID ELISA PROCESSES AND RELATED COMPOSITIONS 有权
    快速ELISA方法和相关组合物

    公开(公告)号：US20100075349A1

    公开(公告)日：2010-03-25

    申请号：US12626327

    申请日：2009-11-25

    申请人： Zhuying WANG ,  Fang Liang Zhang ,  Tao Bai

    发明人： Zhuying WANG ,  Fang Liang Zhang ,  Tao Bai

    IPC分类号： G01N33/533

    CPC分类号： G01N33/54393

    摘要： The present invention provides improved and rapid detection methods for an antigen such as a chemical compound, a peptide, a nucleic acid, or a protein released from cells or virus particles in situ. The detection time for an antigen can be dramatically reduced relative to conventional technologies. The technology can particularly be used, for example, to modify and reduce the detection time significantly in traditional ELISA, and also Western blot or Dot blot assays. The improved ELISA method is rapid, economical, reproducible, simple and automatable. Also provided are compositions and kits for using the improved ELISA methods for the rapid detection of antigens.

    摘要翻译： 本发明提供了一种抗原例如化合物，肽，核酸或原位从细胞或病毒颗粒释放的蛋白质的改进和快速检测方法。 相对于常规技术，抗原的检测时间可以显着降低。 该技术可以特别用于例如在传统ELISA中显着修饰和减少检测时间，以及Western印迹或斑点印迹测定。 改进的ELISA方法快速，经济，可重复，简单，可自动化。 还提供了使用改进的ELISA方法快速检测抗原的组合物和试剂盒。

    RAPID ELISA PROCESSES AND RELATED COMPOSITIONS
    10.
    发明申请
    RAPID ELISA PROCESSES AND RELATED COMPOSITIONS 有权
    快速ELISA方法和相关组合物

    公开(公告)号：US20080311602A1

    公开(公告)日：2008-12-18

    申请号：US12119793

    申请日：2008-05-13

    申请人： Zhuying Wang ,  Fang Liang Zhang ,  Tao Bai

    发明人： Zhuying Wang ,  Fang Liang Zhang ,  Tao Bai

    IPC分类号： G01N33/53

    CPC分类号： G01N33/54393

    摘要： The present invention provides improved and rapid detection methods for an antigen such as a chemical compound, a peptide, a nucleic acid, or a protein released from cells or virus particles in situ. The detection time for an antigen can be dramatically reduced relative to conventional technologies. The technology can particularly be used, for example, to modify and reduce the detection time significantly in traditional ELISA, and also Western blot or Dot blot assays. The improved ELISA method is rapid, economical, reproducible, simple and automatable. Also provided are compositions and kits for using the improved ELISA methods for the rapid detection of antigens.

    摘要翻译： 本发明提供了一种抗原例如化合物，肽，核酸或原位从细胞或病毒颗粒释放的蛋白质的改进和快速检测方法。 相对于常规技术，抗原的检测时间可以显着降低。 该技术可以特别用于例如在传统ELISA中显着修饰和减少检测时间，以及Western印迹或斑点印迹测定。 改进的ELISA方法快速，经济，可重复，简单，可自动化。 还提供了使用改进的ELISA方法快速检测抗原的组合物和试剂盒。