发明申请
US20100178671A1 VECTOR CONSTRUCTS AND METHODS FOR EXPRESSING AND SECRETING POLYPEPTIDES IN FILAMENTOUS FUNGI USING A SELF-PROCESSING 2A CLEAVAGE SITE
审中-公开
使用自加工2A清洁场地在真菌真菌中表达和分泌多肽的载体构建体和方法
- 专利标题： VECTOR CONSTRUCTS AND METHODS FOR EXPRESSING AND SECRETING POLYPEPTIDES IN FILAMENTOUS FUNGI USING A SELF-PROCESSING 2A CLEAVAGE SITE
- 专利标题（中）： 使用自加工2A清洁场地在真菌真菌中表达和分泌多肽的载体构建体和方法
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申请号： US12309365申请日： 2007-07-19
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公开(公告)号： US20100178671A1公开(公告)日： 2010-07-15
- 发明人: Khanh Q. Nguyen , Bruno Winter
- 申请人： Khanh Q. Nguyen , Bruno Winter
- 申请人地址： DE Darmstadt
- 专利权人： AB ENZYMES GMBH
- 当前专利权人： AB ENZYMES GMBH
- 当前专利权人地址： DE Darmstadt
- 优先权： DE102006034124.4 20060724; DE102006052204.4 20061106
- 国际申请： PCT/EP2007/006448 WO 20070719
- 主分类号： C12P21/00
- IPC分类号： C12P21/00 ; C12N15/63 ; C07K2/00 ; C12N1/00 ; C12N1/15
摘要：
The invention relates to a vector construct for expressing and secreting polypeptides in filamentous fungi, comprising, in 5′3′ direction, in functional linkage, optionally a promoter which may have an enhancer placed upstream, at least one DNA sequence which codes for a first polypeptide and which may comprise a signal sequence or not, optionally a DNA sequence which codes for a 2A cleavage site or for a sequence derived therefrom, optionally together with an upstream DNA sequence which codes for a proteolytic cleavage site, or optionally a DNA sequence which codes for a proteolytic cleavage site, a DNA sequence which codes for a second polypeptide with signal sequence, optionally a further DNA sequence which codes for a 2A cleavage site or for a sequence derived therefrom, optionally together with an upstream DNA sequence which codes for a proteolytic cleavage site, or optionally a DNA sequence which codes for a proteolytic cleavage site, optionally a DNA sequence which codes for a third polypeptide with signal sequence, optionally a further DNA sequence which codes for a 2A cleavage site or for a sequence derived therefrom, optionally together with an upstream DNA sequence which codes for a proteolytic cleavage site, or optionally a DNA sequence which codes for a proteolytic cleavage site, optionally a DNA sequence which codes for a fourth polypeptide with signal sequence, a terminator, where at least one 2A cleavage site or a sequence derived therefrom is present in the construct.