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公开(公告)号：US20110287141A1
公开(公告)日：2011-11-24
申请号：US13171005
申请日：2011-06-28
CPC分类号： C12N9/18
摘要： The invention relates to a DNA sequence that encodes a polypeptide with phospholipase activity and was isolated from Aspergillus and sequences derived therefrom, polypeptides with phospholipase activity encoded by these sequences as well as the use of these polypeptides for degumming of vegetable oil, for the preparation of dough and/or bakery products, for the preparation of dairy products, for processing steps in the textile industry and for related applications.
摘要翻译： 本发明涉及一种编码具有磷脂酶活性的多肽的DNA序列，其从曲霉属中分离得到，由此得到的序列由这些序列编码的具有磷脂酶活性的多肽以及这些多肽用于植物油脱胶的用途，用于制备 面团和/或面包店产品，用于制备乳制品，用于纺织工业中的加工步骤和相关应用。
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公开(公告)号：US07993876B2
公开(公告)日：2011-08-09
申请号：US12311406
申请日：2007-09-21
IPC分类号： C12P21/06
CPC分类号： C12N9/18
摘要： The invention relates to a DNA sequence that encodes a polypeptide with phospholipase activity and was isolated from Aspergillus and sequences derived therefrom, polypeptides with phospholipase activity encoded by these sequences as well as the use of these polypeptides for degumming of vegetable oil, for the preparation of dough and/or bakery products, for the preparation of dairy products, for processing steps in the textile industry and for related applications.
摘要翻译： 本发明涉及一种编码具有磷脂酶活性的多肽的DNA序列，其从曲霉属中分离得到，由此得到的序列由这些序列编码的具有磷脂酶活性的多肽以及这些多肽用于植物油脱胶的用途，用于制备 面团和/或面包店产品，用于制备乳制品，用于纺织工业中的加工步骤和相关应用。
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公开(公告)号：US20100004170A1
公开(公告)日：2010-01-07
申请号：US12312357
申请日：2007-11-02
申请人： Bruno Winter , Stephen Cole
发明人： Bruno Winter , Stephen Cole
IPC分类号： A61K38/47 , A61K38/16 , A61K38/43 , A61K38/46 , B05D3/00 , A61P43/00 , A23K1/16 , A23L1/20 , A23K1/14
CPC分类号： A61K9/5089 , A23K10/30 , A23K40/30 , A23L11/07 , A23L29/06 , A23L29/35 , A23P20/10 , A61K9/5063 , A61K38/465 , A61K38/47
摘要： The invention relates to an agent with increased thermal stability comprising a core and a coating layer characterized in that the core comprises at least one biologically active protein and that the coating layer comprises a micronized product from leguminous plants, a method for the production of this agent as well as the use of a micronized product from leguminous plants to increase the thermal stability of biologically active proteins in methods for the production of a granulated product as well as in methods for the production of animal feed, food and pharmaceuticals.
摘要翻译： 本发明涉及具有增加的热稳定性的试剂，其包含芯和涂层，其特征在于所述芯包含至少一种生物活性蛋白，并且所述涂层包含豆科植物的微粉化产物，该制剂的生产方法 以及使用来自豆科植物的微粉化产品以增加生产粒状产物的方法中生物活性蛋白质的热稳定性以及生产动物饲料，食品和药物的方法。
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公开(公告)号：US09109210B2
公开(公告)日：2015-08-18
申请号：US13380694
申请日：2010-07-01
申请人： Stephane Blesa , Helene Chautard , Marc Delcourt , Laurent Mesta , Bruno Winter
发明人： Stephane Blesa , Helene Chautard , Marc Delcourt , Laurent Mesta , Bruno Winter
IPC分类号： C12N9/16
CPC分类号： C12N9/16 , C12Y301/03008 , C12Y301/03026
摘要： The present invention provides the field of enhancing proteins and in particular to that of proteins enhanced by molecular change. It provides a variant of a phytase that is termed enhanced in that is has better thermostability and/or activity than the original phytase. The invention also provides a nucleic acid coding for said variant, a cassette or an expression vector containing said variant, a host cell expressing said variant, a composition comprising said variant and uses thereof, principally in the preparation of food additives and animal feed.
摘要翻译： 本发明提供增强蛋白质的领域，特别是通过分子变化增强蛋白质的领域。 它提供了一种植酸酶的变体，其被称为增强的，其具有比原始植酸酶更好的热稳定性和/或活性。 本发明还提供编码所述变体的核酸，含有所述变体的盒或表达载体，表达所述变体的宿主细胞，包含所述变体的组合物及其用途，主要用于制备食品添加剂和动物饲料。
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公开(公告)号：US08507241B2
公开(公告)日：2013-08-13
申请号：US13468786
申请日：2012-05-10
IPC分类号： C12N9/18
CPC分类号： C12N9/18 , C12Y301/01005
摘要： The invention relates to a DNA sequence that encodes a polypeptide with lysophospholipase activity and was isolated from Aspergillus and sequences derived therefrom, polypeptides with lysophospholipase activity encoded by these sequences as well as the use of these polypeptides for improving the filterability of syrups consisting of wheat starch and for related applications.
摘要翻译： 本发明涉及一种编码具有溶血磷脂酶活性的多肽的DNA序列，并从曲霉中分离得到，由此得到的序列由这些序列编码的具有溶血磷脂酶活性的多肽以及这些多肽用于改善由小麦淀粉组成的糖浆的过滤性的用途 并针对相关应用。
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6.发明申请POLYPEPTIDE HAVING PHYTASE ACTIVITY AND INCREASED TEMPERATURE RESISTANCE OF THE ENZYME ACTIVITY, AND NUCLEOTIDE SEQUENCE CODING SAID POLYPEPTIDE 有权具有酶活性的多肽和酶活性的增加的耐温度和核苷酸序列编码所述的多肽公开(公告)号：US20110262619A1
公开(公告)日：2011-10-27
申请号：US12312381
申请日：2007-11-02
申请人： Khanh Q Nguyen , Bruno Winter
发明人： Khanh Q Nguyen , Bruno Winter
IPC分类号： C12N9/16 , C12N15/63 , C12N5/10 , A23K1/165 , C12N1/15 , C12N1/19 , A23L1/30 , C07H21/04 , C12N1/21
CPC分类号： C12N9/16 , A23K10/14 , A23L29/06 , C12Y301/03026
摘要： The invention relates to a recombinant DNA molecule coding a polypeptide having phytase stability after being expressed in a prokaryotic or eukaryotic host cell. Said recombinant DNA molecule comprises a DNA sequence selected from among a) DNA sequences coding a polypeptide that has phytase activity and is obtained by varying the mature wild-type E. coli phytase sequence, the variation being selected from among i) the mutation lysine→aspartic acid in position 74 (K74D), and/or ii) a combination of the mutations asparagine→arginine in position 139 (N139R) and aspartic acid→glutamic acid in position 142 (D142E), and/or iii) a combination of the mutations leucine→isoleucine in position 145 (L145I) and leucine→isoleucine in position 198 (L198I), and/or iv) a mutation valine→proline in position 200 (V200P), and/or v) an N-terminal or C-terminal or an N-terminal and C-terminal addition of a sequence section of the acidic phosphatase of Aspergillus niger var. awamori or the phytase of Aspergillus niger, b) DNA sequences that are 70 to 100 percent homologous to the sequences listed under a), c) DNA sequences which are related to the sequences listed under a) and b) because of the degeneration of the genetic code. When expressed in a suitable host cell, the recombinant DNA molecule has increased temperature stability as well as increased protease stability of the enzyme activity of the protein coded in said manner. Variations iii) and iv) are provided only in combination with a variation i), ii), and v). The invention also relates to the proteins coded thereby.
摘要翻译： 本发明涉及在原核或真核宿主细胞中表达后，编码具有植酸酶稳定性的多肽的重组DNA分子。 所述重组DNA分子包含选自以下的DNA序列：a）编码具有植酸酶活性的多肽的DNA序列，其通过改变成熟的野生型大肠杆菌肌醇六磷酸酶序列获得，所述变异选自i）赖氨酸→赖氨酸→ 和位置74（K74D）中的天冬氨酸和/或ii）位置139（N139R）中的天冬酰胺→精氨酸与位置142（D142E）中的天冬氨酸→谷氨酸的突变的组合，和/或iii） 和位置200的突变缬氨酸→脯氨酸（V200P），和/或v）N-末端或C-末端的突变缬氨酸→脯氨酸， 末端或N末端和C末端添加的黑曲霉变种的酸性磷酸酶的序列部分。 泡盛曲霉或黑曲霉的植酸酶，b）与a）所列序列具有70至100％同源性的DNA序列，c）与a）和b）下列出的序列相关的DNA序列，因为 遗传密码。 当在合适的宿主细胞中表达时，重组DNA分子具有提高的温度稳定性以及增加以所述方式编码的蛋白质的酶活性的蛋白酶稳定性。 变体iii）和iv）仅与变体i），ii）和v）组合提供。 本发明还涉及由此编码的蛋白质。
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7.发明申请VECTOR CONSTRUCTS AND METHODS FOR EXPRESSING AND SECRETING POLYPEPTIDES IN FILAMENTOUS FUNGI USING A SELF-PROCESSING 2A CLEAVAGE SITE 审中-公开使用自加工2A清洁场地在真菌真菌中表达和分泌多肽的载体构建体和方法公开(公告)号：US20100178671A1
公开(公告)日：2010-07-15
申请号：US12309365
申请日：2007-07-19
申请人： Khanh Q. Nguyen , Bruno Winter
发明人： Khanh Q. Nguyen , Bruno Winter
CPC分类号： C12N15/80 , C07K2319/50 , C12P21/06
摘要： The invention relates to a vector construct for expressing and secreting polypeptides in filamentous fungi, comprising, in 5′3′ direction, in functional linkage, optionally a promoter which may have an enhancer placed upstream, at least one DNA sequence which codes for a first polypeptide and which may comprise a signal sequence or not, optionally a DNA sequence which codes for a 2A cleavage site or for a sequence derived therefrom, optionally together with an upstream DNA sequence which codes for a proteolytic cleavage site, or optionally a DNA sequence which codes for a proteolytic cleavage site, a DNA sequence which codes for a second polypeptide with signal sequence, optionally a further DNA sequence which codes for a 2A cleavage site or for a sequence derived therefrom, optionally together with an upstream DNA sequence which codes for a proteolytic cleavage site, or optionally a DNA sequence which codes for a proteolytic cleavage site, optionally a DNA sequence which codes for a third polypeptide with signal sequence, optionally a further DNA sequence which codes for a 2A cleavage site or for a sequence derived therefrom, optionally together with an upstream DNA sequence which codes for a proteolytic cleavage site, or optionally a DNA sequence which codes for a proteolytic cleavage site, optionally a DNA sequence which codes for a fourth polypeptide with signal sequence, a terminator, where at least one 2A cleavage site or a sequence derived therefrom is present in the construct.
摘要翻译： 本发明涉及用于在丝状真菌中表达和分泌多肽的载体构建体，其在5'3'方向上具有功能性连接，任选地可以具有位于上游的增强子的启动子，至少一个编码第一 多肽，并且其可以包含信号序列或非编码2A切割位点的DNA序列或任选编码来自其的序列的DNA序列，任选地与编码蛋白水解切割位点的上游DNA序列或任选的DNA序列， 编码蛋白水解切割位点的DNA序列，编码具有信号序列的第二多肽的DNA序列，任选地编码2A切割位点的另外的DNA序列或其衍生的序列，任选地与上游DNA序列一起编码， 蛋白水解切割位点或任选编码蛋白水解切割位点的DNA序列，任选地编码编码的DNA序列 具有信号序列的第三多肽，任选地编码2A切割位点的另外的DNA序列或其衍生的序列，任选地与编码蛋白水解切割位点的上游DNA序列或任选的编码一个 蛋白水解切割位点，任选地编码具有信号序列的第四多肽的DNA序列，终止子，其中至少一个2A切割位点或由其衍生的序列存在于构建体中。
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8.发明申请Polypeptide Having Phytase Activity and Nucleotide Sequence Encoding the Same 有权具有植酸酶活性的多肽和编码其的核苷酸序列公开(公告)号：US20090169536A1
公开(公告)日：2009-07-02
申请号：US11665631
申请日：2005-10-14
申请人： Khanh Q. Nguyen , Bruno Winter
发明人： Khanh Q. Nguyen , Bruno Winter
IPC分类号： A61K38/46 , C12N15/11 , C12N9/14 , C12N15/00 , C12N1/21 , C12N1/19 , C12N5/06 , C12P21/02 , A23L1/305
CPC分类号： C12N9/16
摘要： The invention relates to a recombinant DNA molecule, which, upon expression in a prokaryotic or eukaryotic host cell, encodes a polypeptide having phytase activity, wherein the recombinant DNA molecule comprises a DNA sequence selected from a) DNA sequences that have been obtained by variations of the mature wild-type E. coli phytase sequence, wherein at least one amino acid in the region of position 189 to 211 and/or an amino acid in the region of position 137 to 152 is mutated as compared to the wild-type sequence, b) DNA sequences having a homology of 70% to 100% to the sequences according to a), c) DNA sequences that are related to the sequences according to a) and b) due to the degeneracy of the genetic code, wherein the recombinant DNA molecule is, upon expression in a suitable host cell, associated with an increased activity of the thus encoded protein in the culture supernatant, as well as the proteins encoded by the same.
摘要翻译： 本发明涉及一种重组DNA分子，其在原核或真核宿主细胞中表达后，编码具有植酸酶活性的多肽，其中重组DNA分子包含选自以下的DNA序列：a）通过 与野生型序列相比，成熟的野生型大肠杆菌肌醇六磷酸酶序列突变，其中位置189至211的区域中的至少一个氨基酸和/或位置137至152的区域中的氨基酸被突变， b）与根据a）的序列具有70％至100％同源性的DNA序列，c）由于遗传密码的简并性而与根据a）和b）的序列相关的DNA序列，其中重组 DNA分子在合适的宿主细胞中表达时，与培养上清液中由此编码的蛋白质的活性增加以及由其编码的蛋白质相关。
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公开(公告)号：US08822201B2
公开(公告)日：2014-09-02
申请号：US12448482
申请日：2007-12-05
申请人： Jörg Köhler , Volker Marschner , Bruno Winter
发明人： Jörg Köhler , Volker Marschner , Bruno Winter
摘要： The invention relates to a method for recovering of oil from plant seeds characterized in that a) an aqueous solution containing one or more cellulolytic and/or lipolytic and/or pectinolytic and/or proteolytic enzyme(s) and/or phytase is sprayed onto the seed, b) the thus obtained seed is directly supplied to a one-stage or multistage pressing in a way known per se, optionally coupled to an extraction, and c) the oil is recovered in a way known per se and optionally further processed, and the use of the method, particularly in the production of edible oil or biodiesel.
摘要翻译： 本发明涉及从植物种子中回收油的方法，其特征在于：a）将含有一种或多种纤维素分解和/或脂肪分解和/或果胶分解和/或蛋白水解酶和/或植酸酶的水溶液喷雾到 种子，b）将如此获得的种子以本身已知的方式直接供应至一级或多级压榨，任选地与提取物偶联，和c）以本身已知的方式回收油并任选进一步加工， 并采用该方法，特别是在生产食用油或生物柴油时。
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公开(公告)号：US08653241B2
公开(公告)日：2014-02-18
申请号：US13171005
申请日：2011-06-28
IPC分类号： C07K1/00
CPC分类号： C12N9/18
摘要： The invention relates to a DNA sequence that encodes a polypeptide with phospholipase activity and was isolated from Aspergillus and sequences derived therefrom, polypeptides with phospholipase activity encoded by these sequences as well as the use of these polypeptides for degumming of vegetable oil, for the preparation of dough and/or bakery products, for the preparation of dairy products, for processing steps in the textile industry and for related applications.
摘要翻译： 本发明涉及一种编码具有磷脂酶活性的多肽的DNA序列，其从曲霉属中分离得到，由此得到的序列由这些序列编码的具有磷脂酶活性的多肽以及这些多肽用于植物油脱胶的用途，用于制备 面团和/或面包店产品，用于制备乳制品，用于纺织工业中的加工步骤和相关应用。
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