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公开(公告)号：US20110287141A1
公开(公告)日：2011-11-24
申请号：US13171005
申请日：2011-06-28
CPC分类号： C12N9/18
摘要： The invention relates to a DNA sequence that encodes a polypeptide with phospholipase activity and was isolated from Aspergillus and sequences derived therefrom, polypeptides with phospholipase activity encoded by these sequences as well as the use of these polypeptides for degumming of vegetable oil, for the preparation of dough and/or bakery products, for the preparation of dairy products, for processing steps in the textile industry and for related applications.
摘要翻译： 本发明涉及一种编码具有磷脂酶活性的多肽的DNA序列，其从曲霉属中分离得到，由此得到的序列由这些序列编码的具有磷脂酶活性的多肽以及这些多肽用于植物油脱胶的用途，用于制备 面团和/或面包店产品，用于制备乳制品，用于纺织工业中的加工步骤和相关应用。
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公开(公告)号：US07993876B2
公开(公告)日：2011-08-09
申请号：US12311406
申请日：2007-09-21
IPC分类号： C12P21/06
CPC分类号： C12N9/18
摘要： The invention relates to a DNA sequence that encodes a polypeptide with phospholipase activity and was isolated from Aspergillus and sequences derived therefrom, polypeptides with phospholipase activity encoded by these sequences as well as the use of these polypeptides for degumming of vegetable oil, for the preparation of dough and/or bakery products, for the preparation of dairy products, for processing steps in the textile industry and for related applications.
摘要翻译： 本发明涉及一种编码具有磷脂酶活性的多肽的DNA序列，其从曲霉属中分离得到，由此得到的序列由这些序列编码的具有磷脂酶活性的多肽以及这些多肽用于植物油脱胶的用途，用于制备 面团和/或面包店产品，用于制备乳制品，用于纺织工业中的加工步骤和相关应用。
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3.发明申请USE OF PECTINOLYTIC ENZYMES FOR THE TREATMENT OF FRUIT AND VEGETABLE MASH AND ENZYME SEQUENCES THEREFOR 有权用于处理水果和蔬菜MASH的酶解酶用途及其酶解序列公开(公告)号：US20110086133A1
公开(公告)日：2011-04-14
申请号：US12994075
申请日：2009-05-22
申请人： Terhi Puranen , Bernhard Seiboth , Klaudija Milos , Wilfried Theiss , Jarno Kallio , Christian Kubicek , Jari Vehmaanperä
发明人： Terhi Puranen , Bernhard Seiboth , Klaudija Milos , Wilfried Theiss , Jarno Kallio , Christian Kubicek , Jari Vehmaanperä
CPC分类号： C12Y302/01015 , C12N9/2402 , C12Y302/01067
摘要： The invention relates to the use of one or more pectinolytic enzyme(s) for the treatment of fruit or vegetable mash as well as a process for enzymatic treatment of fruit or vegetable mash comprising the step of adding one or more pectinolytic enzyme(s), wherein at least one pectinolytic enzyme is obtainable from Trichoderma reesei, as well as to a process for the preparation of a fruit or vegetable juice comprising the process for enzymatic treatment of fruit or vegetable mash. Moreover, the invention discloses recombinant DNA molecules encoding a polypeptide having endo-polygalacturonase activity, a polypeptide having exo-polygalacturonase activity, a polypeptide having exo-rhamnogalacturonase activity and a polypeptide having xylogalacturonase activity.
摘要翻译： 本发明涉及一种或多种果胶分解酶用于处理水果或蔬菜醪液的用途以及用于酶或处理水果或蔬菜醪液的方法，包括添加一种或多种果胶分解酶， 其中至少一种果胶分解酶可从里氏木霉获得，以及一种用于制备水果或蔬菜汁的方法，其包括用于酶处理水果或蔬菜糊状物的方法。 此外，本发明公开了编码具有内切多聚半乳糖醛酸酶活性的多肽的重组DNA分子，具有外切多聚半乳糖醛酸酶活性的多肽，具有外分泌半乳糖醛酸聚糖酶活性的多肽和具有木糖聚半乳糖醛酸酶活性的多肽。
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公开(公告)号：US20100004170A1
公开(公告)日：2010-01-07
申请号：US12312357
申请日：2007-11-02
申请人： Bruno Winter , Stephen Cole
发明人： Bruno Winter , Stephen Cole
IPC分类号： A61K38/47 , A61K38/16 , A61K38/43 , A61K38/46 , B05D3/00 , A61P43/00 , A23K1/16 , A23L1/20 , A23K1/14
CPC分类号： A61K9/5089 , A23K10/30 , A23K40/30 , A23L11/07 , A23L29/06 , A23L29/35 , A23P20/10 , A61K9/5063 , A61K38/465 , A61K38/47
摘要： The invention relates to an agent with increased thermal stability comprising a core and a coating layer characterized in that the core comprises at least one biologically active protein and that the coating layer comprises a micronized product from leguminous plants, a method for the production of this agent as well as the use of a micronized product from leguminous plants to increase the thermal stability of biologically active proteins in methods for the production of a granulated product as well as in methods for the production of animal feed, food and pharmaceuticals.
摘要翻译： 本发明涉及具有增加的热稳定性的试剂，其包含芯和涂层，其特征在于所述芯包含至少一种生物活性蛋白，并且所述涂层包含豆科植物的微粉化产物，该制剂的生产方法 以及使用来自豆科植物的微粉化产品以增加生产粒状产物的方法中生物活性蛋白质的热稳定性以及生产动物饲料，食品和药物的方法。
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公开(公告)号：US20220354132A1
公开(公告)日：2022-11-10
申请号：US17637973
申请日：2020-08-27
申请人： AB Enzymes GmbH
IPC分类号： A21D8/04
摘要： The invention relates to the use of a composition comprising an effective amount of at least one polypeptide having GH12 cellulase activity optionally together with conventionally used baking additives for improving at least one property of a dough comprising rye flour and/or of a bakery product prepared from said dough as well as to a method of preparing a bakery product based on rye having at least one improved property, said method comprising (i) providing a dough that has been added an effective amount of at least one polypeptide having GH12 cellulase activity or a composition comprising at least one polypeptide having GH12 cellulase activity optionally together with conventionally used baking additives, and rye flour, (ii) baking the dough for a time and temperature sufficient to yield the bakery product as well as to a baking enzyme composition for preparing rye-based bakery products comprising a polypeptide having a GH12 cellulase activity optionally together with one or more dough(s) or bakery product additives.
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公开(公告)号：US09920384B2
公开(公告)日：2018-03-20
申请号：US14758238
申请日：2013-12-23
申请人： AB Enzymes GmbH
CPC分类号： C12Q1/6895 , C07K14/37 , C12N1/14 , C12N15/80 , C12Q2600/156 , C12R1/885
摘要： The invention relates to a process for identifying gene(s)/genetic element(s) associated with mating impairment in strains of Trichoderma reesei QM6a or strains derived thereof comprising the steps of a) providing a first strain being a Trichoderma reesei QM6a strain having a MAT1-2 locus or a strain derived thereof, b) sexually crossing said strain with a second strain being a mating competent strain of a Trichoderma reesei (Hypocrea jecorina) strain having a complementary locus, i.e. the MAT1-1 locus, c) repeatedly back-crossing the MAT1-1 progenies from the crossing of b) or the back-crossing thereof with the first strain of a), until a strain is obtained that is substantially identical to the first Trichoderma reesei QM6a strain or a strain derived thereof, but carries the MAT1-1 locus and is mating competent for crossing with Trichoderma reesei QM6a or any of its MAT1-2 progeny, d) selecting the progeny from step c) that is mating competent for crossing with a Trichoderma reesei (Hypocrea jecorina) having a MAT1-2 locus, and e) identifying the gene(s)/genetic element(s) associated with mating impairment by comparing the genome of the progenies selected in step d) with the genome sequences of the first strain of a) whereby said gene(s)/genetic element(s) may be fully or partially missing or existing in a mutated form or in a form having deletions or insertions in the first strain thus being a gene or a genetic element directly or indirectly associated with mating impairment in strains of Trichoderma reesei QM6a or a strain derived thereof as well as to a process for correcting the mating impairment of a Trichoderma reesei QM6a strain or a strain derived thereof having a MAT1-1 locus and that is not competent to mate with a Trichoderma reesei QM6a strain having a MAT1-2 locus or a strain derived thereof, wherein one or more mutated or fully or partially missing gene(s) and/or genetic element(s) identified as above is/are replaced by or complemented with the corresponding functional gene(s) and/or genetic element(s). Moreover, the invention relates to the use of a thus obtained fungal strain of the genus Trichoderma in industrial breeding and production of a product of interest. Moreover, the invention relates to the genes associated with mating impairment of Trichoderma reesei QM6a and strains derived therefrom and to genes essential for mating of Trichoderma reesei QM6a and strains derived therefrom.
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公开(公告)号：US09353357B2
公开(公告)日：2016-05-31
申请号：US13846155
申请日：2013-03-18
申请人： AB Enzymes GmbH
发明人： Khanh Q. Nguyen , Bruno Winter
CPC分类号： C12N9/16 , A23K10/14 , A23L29/06 , C12Y301/03026
摘要： The invention relates to a recombinant DNA molecule encoding a polypeptide having phytase activity and increased temperature stability and increased proteolytic stability of the enzyme activity. The DNA sequence has been obtained by variation of the mature wild-type E. coli phytase sequence with defined amino acid positions being modified in comparison to the wild-type sequence or with the sequences having N- and/or C-terminal extensions, respectively. The invention further relates to a method for expressing the recombinant phytase as well as its use in the food and animal feed technologies.
摘要翻译： 本发明涉及编码具有植酸酶活性和增加的温度稳定性以及增加的酶活性的蛋白水解稳定性的多肽的重组DNA分子。 DNA序列是通过变异成熟的野生型大肠杆菌肌醇六磷酸酶序列得到的，其具有与野生型序列相比修饰的具有限定的氨基酸位置或与分别具有N-和/或C-末端延伸的序列 。 本发明还涉及用于表达重组植酸酶的方法及其在食物和动物饲料技术中的应用。
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公开(公告)号：US20150037875A1
公开(公告)日：2015-02-05
申请号：US14233625
申请日：2012-07-13
CPC分类号： C12N1/063 , C12G1/00 , C12G1/0203 , C12G1/06 , C12N9/2428
摘要： The invention relates to the use of a composition comprising at least one polypeptide having glucoamylase activity and optionally at least one polypeptide having β-1,3-endoglucanase activity in a process of lysing yeast cell walls as well as to a process for lysing yeast cell walls which comprises exposing said yeast cell walls to a composition comprising at least one polypeptide having glucoamylase activity and optionally at least one polypeptide having β-1,3-endoglucanase activity.
摘要翻译： 本发明涉及包含至少一种具有葡糖淀粉酶活性的多肽和任选的至少一种具有β-内切葡聚糖酶活性的多肽的组合物在裂解酵母细胞壁的过程中的用途以及用于裂解酵母的方法 细胞壁，其包括将所述酵母细胞壁暴露于包含至少一种具有葡糖淀粉酶活性的多肽和任选至少一种具有β-内切葡聚糖酶活性的多肽的组合物。
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公开(公告)号：US08507241B2
公开(公告)日：2013-08-13
申请号：US13468786
申请日：2012-05-10
IPC分类号： C12N9/18
CPC分类号： C12N9/18 , C12Y301/01005
摘要： The invention relates to a DNA sequence that encodes a polypeptide with lysophospholipase activity and was isolated from Aspergillus and sequences derived therefrom, polypeptides with lysophospholipase activity encoded by these sequences as well as the use of these polypeptides for improving the filterability of syrups consisting of wheat starch and for related applications.
摘要翻译： 本发明涉及一种编码具有溶血磷脂酶活性的多肽的DNA序列，并从曲霉中分离得到，由此得到的序列由这些序列编码的具有溶血磷脂酶活性的多肽以及这些多肽用于改善由小麦淀粉组成的糖浆的过滤性的用途 并针对相关应用。
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10.发明申请POLYPEPTIDE HAVING PHYTASE ACTIVITY AND INCREASED TEMPERATURE RESISTANCE OF THE ENZYME ACTIVITY, AND NUCLEOTIDE SEQUENCE CODING SAID POLYPEPTIDE 有权具有酶活性的多肽和酶活性的增加的耐温度和核苷酸序列编码所述的多肽公开(公告)号：US20110262619A1
公开(公告)日：2011-10-27
申请号：US12312381
申请日：2007-11-02
申请人： Khanh Q Nguyen , Bruno Winter
发明人： Khanh Q Nguyen , Bruno Winter
IPC分类号： C12N9/16 , C12N15/63 , C12N5/10 , A23K1/165 , C12N1/15 , C12N1/19 , A23L1/30 , C07H21/04 , C12N1/21
CPC分类号： C12N9/16 , A23K10/14 , A23L29/06 , C12Y301/03026
摘要： The invention relates to a recombinant DNA molecule coding a polypeptide having phytase stability after being expressed in a prokaryotic or eukaryotic host cell. Said recombinant DNA molecule comprises a DNA sequence selected from among a) DNA sequences coding a polypeptide that has phytase activity and is obtained by varying the mature wild-type E. coli phytase sequence, the variation being selected from among i) the mutation lysine→aspartic acid in position 74 (K74D), and/or ii) a combination of the mutations asparagine→arginine in position 139 (N139R) and aspartic acid→glutamic acid in position 142 (D142E), and/or iii) a combination of the mutations leucine→isoleucine in position 145 (L145I) and leucine→isoleucine in position 198 (L198I), and/or iv) a mutation valine→proline in position 200 (V200P), and/or v) an N-terminal or C-terminal or an N-terminal and C-terminal addition of a sequence section of the acidic phosphatase of Aspergillus niger var. awamori or the phytase of Aspergillus niger, b) DNA sequences that are 70 to 100 percent homologous to the sequences listed under a), c) DNA sequences which are related to the sequences listed under a) and b) because of the degeneration of the genetic code. When expressed in a suitable host cell, the recombinant DNA molecule has increased temperature stability as well as increased protease stability of the enzyme activity of the protein coded in said manner. Variations iii) and iv) are provided only in combination with a variation i), ii), and v). The invention also relates to the proteins coded thereby.
摘要翻译： 本发明涉及在原核或真核宿主细胞中表达后，编码具有植酸酶稳定性的多肽的重组DNA分子。 所述重组DNA分子包含选自以下的DNA序列：a）编码具有植酸酶活性的多肽的DNA序列，其通过改变成熟的野生型大肠杆菌肌醇六磷酸酶序列获得，所述变异选自i）赖氨酸→赖氨酸→ 和位置74（K74D）中的天冬氨酸和/或ii）位置139（N139R）中的天冬酰胺→精氨酸与位置142（D142E）中的天冬氨酸→谷氨酸的突变的组合，和/或iii） 和位置200的突变缬氨酸→脯氨酸（V200P），和/或v）N-末端或C-末端的突变缬氨酸→脯氨酸， 末端或N末端和C末端添加的黑曲霉变种的酸性磷酸酶的序列部分。 泡盛曲霉或黑曲霉的植酸酶，b）与a）所列序列具有70至100％同源性的DNA序列，c）与a）和b）下列出的序列相关的DNA序列，因为 遗传密码。 当在合适的宿主细胞中表达时，重组DNA分子具有提高的温度稳定性以及增加以所述方式编码的蛋白质的酶活性的蛋白酶稳定性。 变体iii）和iv）仅与变体i），ii）和v）组合提供。 本发明还涉及由此编码的蛋白质。
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