发明申请
- 专利标题: Nucleic acid amplification reaction station for disposable test devices
- 专利标题(中): 一次性试验装置的核酸扩增反应站
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申请号: US12924351申请日: 2010-09-24
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公开(公告)号: US20110020921A1公开(公告)日: 2011-01-27
- 发明人: Bryan W. Kluttz , Geoff A. McKinley , Fabio Gennari , Michel Guy , Christopher Cotter , Luigi Catanzariti , Louis Graziano , Bruno Colin , Cecile Paris , Jacques Dachaud
- 申请人: Bryan W. Kluttz , Geoff A. McKinley , Fabio Gennari , Michel Guy , Christopher Cotter , Luigi Catanzariti , Louis Graziano , Bruno Colin , Cecile Paris , Jacques Dachaud
- 申请人地址: US NC Durham
- 专利权人: bioMerieux, Inc.
- 当前专利权人: bioMerieux, Inc.
- 当前专利权人地址: US NC Durham
- 主分类号: C12M1/34
- IPC分类号: C12M1/34
摘要:
An instrument for conducting nucleic acid amplification reactions in a disposable test device. The test device includes a first reaction chamber containing a first nucleic acid amplification reagent (e.g., primers and nucleotides) and a second reaction chamber either containing, or in fluid communication, with a second nucleic acid amplification reagent (e.g., an amplification enzyme such as RT). The instrument includes a support structure receiving the test device. A temperature control system maintains the first reaction chamber at a first elevated temperature but simultaneously maintains the second nucleic acid amplification reagent at a second temperature lower than the first temperature so as to preserve the second nucleic acid amplification reagent. An actuator operates on a fluid conduit in the test device to place the first and second reaction chambers in fluid communication with each other after a reaction has occurred in the first reaction chamber at the first temperature. A pneumatic system is also provided that assists in fluid transfer of a reaction solution from the first chamber to the second chamber.
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