公开(公告)号：US20110020921A1

公开(公告)日：2011-01-27

申请号：US12924351

申请日：2010-09-24

申请人： Bryan W. Kluttz ,  Geoff A. McKinley ,  Fabio Gennari ,  Michel Guy ,  Christopher Cotter ,  Luigi Catanzariti ,  Louis Graziano ,  Bruno Colin ,  Cecile Paris ,  Jacques Dachaud

发明人： Bryan W. Kluttz ,  Geoff A. McKinley ,  Fabio Gennari ,  Michel Guy ,  Christopher Cotter ,  Luigi Catanzariti ,  Louis Graziano ,  Bruno Colin ,  Cecile Paris ,  Jacques Dachaud

IPC分类号： C12M1/34

CPC分类号： C12Q1/6848 ,  B01L3/502 ,  B01L7/52 ,  B01L7/525 ,  B01L2200/026 ,  B01L2200/16 ,  B01L2300/044 ,  B01L2300/0809 ,  B01L2300/0825 ,  B01L2300/0861 ,  B01L2300/087 ,  B01L2300/1822 ,  B01L2400/0487 ,  B01L2400/049 ,  B01L2400/0633 ,  B01L2400/0644 ,  B01L2400/0655 ,  G01N35/026 ,  G01N2035/0436

摘要： An instrument for conducting nucleic acid amplification reactions in a disposable test device. The test device includes a first reaction chamber containing a first nucleic acid amplification reagent (e.g., primers and nucleotides) and a second reaction chamber either containing, or in fluid communication, with a second nucleic acid amplification reagent (e.g., an amplification enzyme such as RT). The instrument includes a support structure receiving the test device. A temperature control system maintains the first reaction chamber at a first elevated temperature but simultaneously maintains the second nucleic acid amplification reagent at a second temperature lower than the first temperature so as to preserve the second nucleic acid amplification reagent. An actuator operates on a fluid conduit in the test device to place the first and second reaction chambers in fluid communication with each other after a reaction has occurred in the first reaction chamber at the first temperature. A pneumatic system is also provided that assists in fluid transfer of a reaction solution from the first chamber to the second chamber.

摘要翻译： 用于在一次性测试装置中进行核酸扩增反应的仪器。 测试装置包括含有第一核酸扩增试剂（例如引物和核苷酸）的第一反应室和含有或与第二核酸扩增试剂（例如，扩增酶如 RT）。 仪器包括接收测试装置的支撑结构。 温度控制系统将第一反应室保持在第一升高温度，同时将第二核酸扩增试剂保持在低于第一温度的第二温度，以便保留第二核酸扩增试剂。 致动器在测试装置中的流体导管上操作，以在第一温度下在第一反应室中发生反应之后使第一和第二反应室彼此流体连通。 还提供了一种有助于将反应溶液从第一室流体转移到第二室的气动系统。

公开(公告)号：US06429007B1

公开(公告)日：2002-08-06

申请号：US09420140

申请日：1999-10-18

申请人： Bryan W. Kluttz ,  Geoff A. McKinley ,  Fabio Gennari ,  Michel Guy ,  Christopher Cotter ,  Luigi Catanzariti ,  Louis Graziano ,  Bruno Colin ,  Cecile Jaravel ,  Jacques Dachaud

发明人： Bryan W. Kluttz ,  Geoff A. McKinley ,  Fabio Gennari ,  Michel Guy ,  Christopher Cotter ,  Luigi Catanzariti ,  Louis Graziano ,  Bruno Colin ,  Cecile Jaravel ,  Jacques Dachaud

IPC分类号： C12M136

CPC分类号： C12Q1/6848 ,  B01L3/502 ,  B01L7/52 ,  B01L7/525 ,  B01L2200/026 ,  B01L2200/16 ,  B01L2300/044 ,  B01L2300/0809 ,  B01L2300/0825 ,  B01L2300/0861 ,  B01L2300/087 ,  B01L2300/1822 ,  B01L2400/0487 ,  B01L2400/049 ,  B01L2400/0633 ,  B01L2400/0644 ,  B01L2400/0655 ,  G01N35/026 ,  G01N2035/0436

摘要： An instrument for conducting nucleic acid amplification reactions in a disposable test device. The test device includes a first reaction chamber containing a first nucleic acid amplification reagent (e.g., primers and nucleotides) and a second reaction chamber either containing, or in fluid communication, with a second nucleic acid amplification reagent (e.g., an amplification enzyme such as RT). The instrument includes a support structure receiving the test device. A temperature control system maintains the first reaction chamber at a first elevated temperature but simultaneously maintains the second nucleic acid amplification reagent at a second temperature lower than the first temperature so as to preserve the second nucleic acid amplification reagent. An actuator operates on a fluid conduit in the test device to place the first and second reaction chambers in fluid communication with each other after a reaction has occurred in the first reaction chamber at the first temperature. A pneumatic system is also provided that assists in fluid transfer of a reaction solution from the first chamber to the second chamber.

摘要翻译： 用于在一次性测试装置中进行核酸扩增反应的仪器。 测试装置包括含有第一核酸扩增试剂（例如引物和核苷酸）的第一反应室和含有或与第二核酸扩增试剂（例如，扩增酶如 RT）。 仪器包括接收测试装置的支撑结构。 温度控制系统将第一反应室保持在第一升高温度，同时将第二核酸扩增试剂保持在低于第一温度的第二温度，以便保留第二核酸扩增试剂。 致动器在测试装置中的流体导管上操作，以在第一温度下在第一反应室中发生反应之后使第一和第二反应室彼此流体连通。 还提供了一种有助于将反应溶液从第一室流体转移到第二室的气动系统。

公开(公告)号：US06949376B2

公开(公告)日：2005-09-27

申请号：US10141049

申请日：2002-05-07

申请人： Bryan W. Kluttz ,  Geoff A. McKinley ,  Fabio Gennari ,  Michel Guy ,  Christopher Cotter ,  Luigi Catanzariti ,  Louis Graziano ,  Bruno Colin ,  Cecile Jaravel ,  Jacques Dachaud

发明人： Bryan W. Kluttz ,  Geoff A. McKinley ,  Fabio Gennari ,  Michel Guy ,  Christopher Cotter ,  Luigi Catanzariti ,  Louis Graziano ,  Bruno Colin ,  Cecile Jaravel ,  Jacques Dachaud

IPC分类号： C12N15/09 ,  B01J19/00 ,  B01J19/24 ,  B01L3/00 ,  B01L7/00 ,  C12M20060101 ,  C12M1/00 ,  C12M1/18 ,  C12M1/34 ,  C12M3/00 ,  C12Q1/68 ,  G01N21/00 ,  G01N33/50 ,  G01N35/02 ,  G01N37/00 ,  C12M1/36

CPC分类号： C12Q1/6848 ,  B01L3/502 ,  B01L7/52 ,  B01L7/525 ,  B01L2200/026 ,  B01L2200/16 ,  B01L2300/044 ,  B01L2300/0809 ,  B01L2300/0825 ,  B01L2300/0861 ,  B01L2300/087 ,  B01L2300/1822 ,  B01L2400/0487 ,  B01L2400/049 ,  B01L2400/0633 ,  B01L2400/0644 ,  B01L2400/0655 ,  G01N35/026 ,  G01N2035/0436

摘要： An instrument for conducting nucleic acid amplification reactions in a disposable test device. The test device includes a first reaction chamber containing a first nucleic acid amplification reagent (e.g., primers and nucleotides) and a second reaction chamber either containing, or in fluid communication, with a second nucleic acid amplification reagent (e.g., an amplification enzyme such as RT). The instrument includes a support structure receiving the test device. A temperature control system maintains the first reaction chamber at a first elevated temperature but simultaneously maintains the second nucleic acid amplification reagent at a second temperature lower than the first temperature so as to preserve the second nucleic acid amplification reagent. An actuator operates on a fluid conduit in the test device to place the first and second reaction chambers in fluid communication with each other after a reaction has occurred in the first reaction chamber at the first temperature. A pneumatic system is also provided that assists in fluid transfer of a reaction solution from the first chamber to the second chamber.

公开(公告)号：US07214529B2

公开(公告)日：2007-05-08

申请号：US11180806

申请日：2005-07-12

申请人： Bryan W. Kluttz ,  Geoff A. McKinley ,  Fabio Gennari ,  Michel Guy ,  Christopher Cotter ,  Luigi Catanzariti ,  Louis Graziano ,  Bruno Colin ,  Cecile Jaravel ,  Jacques Dachaud

发明人： Bryan W. Kluttz ,  Geoff A. McKinley ,  Fabio Gennari ,  Michel Guy ,  Christopher Cotter ,  Luigi Catanzariti ,  Louis Graziano ,  Bruno Colin ,  Cecile Jaravel ,  Jacques Dachaud

IPC分类号： C12M1/36

CPC分类号： C12Q1/6848 ,  B01L3/502 ,  B01L7/52 ,  B01L7/525 ,  B01L2200/026 ,  B01L2200/16 ,  B01L2300/044 ,  B01L2300/0809 ,  B01L2300/0825 ,  B01L2300/0861 ,  B01L2300/087 ,  B01L2300/1822 ,  B01L2400/0487 ,  B01L2400/049 ,  B01L2400/0633 ,  B01L2400/0644 ,  B01L2400/0655 ,  G01N35/026 ,  G01N2035/0436

摘要： An instrument for conducting nucleic acid amplification reactions in a disposable test device. The test device includes a first reaction chamber containing a first nucleic acid amplification reagent (e.g., primers and nucleotides) and a second reaction chamber either containing, or in fluid communication, with a second nucleic acid amplification reagent (e.g., an amplification enzyme such as RT). The instrument includes a support structure receiving the test device. A temperature control system maintains the first reaction chamber at a first elevated temperature but simultaneously maintains the second nucleic acid amplification reagent at a second temperature lower than the first temperature so as to preserve the second nucleic acid amplification reagent. An actuator operates on a fluid conduit in the test device to place the first and second reaction chambers in fluid communication with each other after a reaction has occurred in the first reaction chamber at the first temperature. A pneumatic system is also provided that assists in fluid transfer of a reaction solution from the first chamber to the second chamber.

摘要翻译： 用于在一次性测试装置中进行核酸扩增反应的仪器。 测试装置包括含有第一核酸扩增试剂（例如引物和核苷酸）的第一反应室和含有或与第二核酸扩增试剂（例如，扩增酶如 RT）。 仪器包括接收测试装置的支撑结构。 温度控制系统将第一反应室保持在第一升高温度，同时将第二核酸扩增试剂保持在低于第一温度的第二温度，以便保留第二核酸扩增试剂。 致动器在测试装置中的流体导管上操作，以在第一温度下在第一反应室中发生反应之后使第一和第二反应室彼此流体连通。 还提供了一种有助于将反应溶液从第一室流体转移到第二室的气动系统。

公开(公告)号：US20050244887A1

公开(公告)日：2005-11-03

申请号：US11180806

申请日：2005-07-12

申请人： Bryan Kluttz ,  Geoff McKinley ,  Fabio Gennari ,  Michel Guy ,  Christopher Cotter ,  Luigi Catanzariti ,  Louis Graziano ,  Bruno Colin ,  Cecile Jaravel ,  Jacques Dachaud

发明人： Bryan Kluttz ,  Geoff McKinley ,  Fabio Gennari ,  Michel Guy ,  Christopher Cotter ,  Luigi Catanzariti ,  Louis Graziano ,  Bruno Colin ,  Cecile Jaravel ,  Jacques Dachaud

IPC分类号： C12N15/09 ,  B01J19/00 ,  B01J19/24 ,  B01L3/00 ,  B01L7/00 ,  C12M20060101 ,  C12M1/00 ,  C12M1/18 ,  C12M1/34 ,  C12M3/00 ,  C12Q1/68 ,  G01N21/00 ,  G01N33/50 ,  G01N35/02 ,  G01N37/00

CPC分类号： C12Q1/6848 ,  B01L3/502 ,  B01L7/52 ,  B01L7/525 ,  B01L2200/026 ,  B01L2200/16 ,  B01L2300/044 ,  B01L2300/0809 ,  B01L2300/0825 ,  B01L2300/0861 ,  B01L2300/087 ,  B01L2300/1822 ,  B01L2400/0487 ,  B01L2400/049 ,  B01L2400/0633 ,  B01L2400/0644 ,  B01L2400/0655 ,  G01N35/026 ,  G01N2035/0436

摘要： An instrument for conducting nucleic acid amplification reactions in a disposable test device. The test device includes a first reaction chamber containing a first nucleic acid amplification reagent (e.g., primers and nucleotides) and a second reaction chamber either containing, or in fluid communication, with a second nucleic acid amplification reagent (e.g., an amplification enzyme such as RT). The instrument includes a support structure receiving the test device. A temperature control system maintains the first reaction chamber at a first elevated temperature but simultaneously maintains the second nucleic acid amplification reagent at a second temperature lower than the first temperature so as to preserve the second nucleic acid amplification reagent. An actuator operates on a fluid conduit in the test device to place the first and second reaction chambers in fluid communication with each other after a reaction has occurred in the first reaction chamber at the first temperature. A pneumatic system is also provided that assists in fluid transfer of a reaction solution from the first chamber to the second chamber.

摘要翻译： 用于在一次性测试装置中进行核酸扩增反应的仪器。 测试装置包括含有第一核酸扩增试剂（例如引物和核苷酸）的第一反应室和含有或与第二核酸扩增试剂（例如，扩增酶如 RT）。 仪器包括接收测试装置的支撑结构。 温度控制系统将第一反应室保持在第一升高温度，同时将第二核酸扩增试剂保持在低于第一温度的第二温度，以便保留第二核酸扩增试剂。 致动器在测试装置中的流体导管上操作，以在第一温度下在第一反应室中发生反应之后使第一和第二反应室彼此流体连通。 还提供了一种有助于将反应溶液从第一室流体转移到第二室的气动系统。

公开(公告)号：US20060078466A1

公开(公告)日：2006-04-13

申请号：US11251758

申请日：2005-10-18

申请人： Bruno Colin ,  Jacques Dachaud ,  Marie Privat ,  Cecile Paris

发明人： Bruno Colin ,  Jacques Dachaud ,  Marie Privat ,  Cecile Paris

IPC分类号： G01N15/06

CPC分类号： G01N35/1097 ,  B01L3/5025

摘要： The invention concerns a device for implementing a test card inside which sequential reactions and fluid transfer operations are performed under the effect of a control means integrated into the card; said card includes at least two sequential reaction systems arranged in parallel, each reaction system consisting of at least two fluid transfer systems arranged in series. The invention also concerns a test card and the method for implementing the device and the card. The device is characterized in that it contains at least one actuator per reaction line, and all the actuators are at a regular distance from one another, with the actuators preferably being equidistant. The invention is particularly applicable for the micromanipulation of fluids in biological applications.

摘要翻译： 本发明涉及一种用于实现测试卡的设备，其中在集成到卡中的控制装置的作用下执行顺序反应和流体传递操作; 所述卡包括平行布置的至少两个连续的反应系统，每个反应系统由串联布置的至少两个流体传输系统组成。 本发明还涉及测试卡和用于实现设备和卡的方法。 该装置的特征在于，其每个反应管线包含至少一个致动器，并且所有致动器彼此间隔一定距离，致动器优选为等距离的。 本发明特别适用于生物应用中流体的显微操作。

公开(公告)号：US07491497B2

公开(公告)日：2009-02-17

申请号：US11251758

申请日：2005-10-18

申请人： Bruno Colin ,  Jacques Dachaud ,  Marie Privat ,  Cécile Paris

发明人： Bruno Colin ,  Jacques Dachaud ,  Marie Privat ,  Cécile Paris

IPC分类号： C12Q1/68 ,  G01N21/00

CPC分类号： G01N35/1097 ,  B01L3/5025

摘要： A device for implementing a test card inside which sequential reactions and fluid transfer operations are performed under the effect of a control integrated into the card; the card includes at least two sequential reaction systems arranged in parallel, each reaction system having at least two fluid transfer systems arranged in series. A test card and the method for implementing the device and the card are also disclosed. The device contains at least one actuator per reaction line, and all the actuators are at a regular distance from one another, with the actuators preferably being equidistant. The invention is particularly applicable for the micromanipulation of fluids in biological applications.

摘要翻译： 一种用于实现测试卡的装置，其中在集成到卡中的控制的作用下执行顺序反应和流体转移操作; 卡片包括平行布置的至少两个顺序反应系统，每个反应系统具有串联布置的至少两个流体转移系统。 还公开了一种测试卡和用于实现设备和卡的方法。 该装置每个反应管线包含至少一个致动器，并且所有致动器彼此间隔一定距离，致动器优选等距。 本发明特别适用于生物应用中流体的显微操作。

公开(公告)号：US06929239B1

公开(公告)日：2005-08-16

申请号：US09786617

申请日：1999-09-08

申请人： Bruno Colin ,  Cécile Paris ,  Jacques Dachaud ,  Pierre Imbaud

发明人： Bruno Colin ,  Cécile Paris ,  Jacques Dachaud ,  Pierre Imbaud

IPC分类号： G01N1/00 ,  B01J19/00 ,  B01L3/00 ,  C12Q1/68 ,  F15C3/00 ,  F16K1/14 ,  F16K1/16 ,  F16K11/00

CPC分类号： F16K99/0001 ,  B01L3/502738 ,  B01L2300/0819 ,  B01L2300/0887 ,  B01L2300/123 ,  B01L2400/0605 ,  B01L2400/0638 ,  F16K99/0015 ,  F16K99/0023 ,  F16K2099/0071

摘要： The invention concerns a device for performing a reaction or at least two reactions in parallel or in series, and for transferring a fluid flow, consisting of. an upper surface (2) and a lower surface (3) mutually linked by an edge (4); at least a duct (5) wherein a fluid flow (F1) can be generated by means transferring at least one sample (6) to be treated and/or analysed, and at least a valve (8) incorporated in each duct (4) for directing each sample (6) thereby controlling the transfers, reactions and analyses in the device. Each valve (8) consists of two means (9 and 10) integral with the device, the first means (9) being mobile between an inoperative position wherein the fluid transfer (6) is possible and an active position wherein said transfer cannot take place, and the second means (10) being mobile between a position wherein it is not in contact with said first means (9) and an active position wherein it deforms the first means (9) and it is activated by an actuator external to the device.

摘要翻译： 本发明涉及一种用于并行或串联地进行反应或至少两个反应的装置，并用于传送由以下组成的流体流。 由边缘（4）相互连接的上表面（2）和下表面（3）; 至少一个管道（5），其中可以通过传送至少一个待处理和/或分析的样品（6）的装置产生流体流（F 1），以及至少一个结合在每个管道（4）中的阀门 ），用于引导每个样品（6），从而控制装置中的转移，反应和分析。 每个阀（8）由与装置成一体的两个装置（9和10）组成，第一装置（9）可移动在不可操作的位置，其中流体传送（6）是可能的，并且其中所述传送不能发生的活动位置 ，并且所述第二装置（10）在其不与所述第一装置（9）接触的位置和其中使所述第一装置（9）变形并且由所述装置外部的致动器启动的活动位置之间可移动 。

公开(公告)号：US06902706B1

公开(公告)日：2005-06-07

申请号：US10009824

申请日：2000-06-21

申请人： Bruno Colin ,  Jacques Dachaud

发明人： Bruno Colin ,  Jacques Dachaud

IPC分类号： G01N1/00 ,  B01L3/00 ,  F15C5/00 ,  F16K7/12 ,  F16K99/00 ,  G01N35/00 ,  B01L11/00

CPC分类号： F16K99/0046 ,  B01L3/502738 ,  B01L2300/0816 ,  B01L2400/0406 ,  B01L2400/0481 ,  B01L2400/0655 ,  F16K99/0001 ,  F16K99/0015 ,  F16K2099/008 ,  G01N2035/00148 ,  G01N2035/00247

摘要： A valve (2), crossed by at least one channel (3), allowing to direct at least one fluid (F5) displaced by a transfer device within a test sample card (1), the card (1) featuring two faces (4 and 5) connected to one another (4) (5) by an edge (6). A card equipped with a device allowing several cards equipped with such valves to be implemented is made up of a flexible film (7), and/or which can be distorted, part of which is fixed to at least one of the faces (4 and/or 5) of said card (1) on the one hand, and a film compression device (7), which can be deactivated on the other hand. In addition, securing is carried out on at least one of the plane faces (4 and/or 5), for example, by a securing device located at the level of a recession (9) peripheral to the valve (2), such as a groove (9). The valve is particularly applicable in the field of diagnosis.

摘要翻译： 通过至少一个通道（3）交叉的阀（2）允许在测试样品卡（1）内引导由转移装置移位的至少一种流体（F 5），所述卡（1）具有两个面 4和5）通过边缘（6）彼此连接（4）（5）。 配备有允许配备有这种阀的几个卡的装置的卡由柔性膜（7）构成，和/或可以变形，其中一部分被固定到至少一个面（4和 /或5），以及胶片压缩装置（7），另一方面可以被禁用。 此外，例如通过位于阀（2）周边的凹部（9）的水平面处的固定装置，在平面（4和/或5）中的至少一个上进行固定，例如 凹槽（9）。 该阀特别适用于诊断领域。