Invention Application
US20120015380A1 ANTI-POLYETHYLENE GLYCOL ANTIBODY EXPRESSING CELL QUANTIFY ANY FREE POLYETHYLENE GLYCOL AND POLYETHYLENE GLYCOL-DERIVATIZED MOLECULES
审中-公开
抗聚乙二醇抗体表达细胞定量任意的聚乙烯甘油和聚乙二醇衍生的分子
- Patent Title: ANTI-POLYETHYLENE GLYCOL ANTIBODY EXPRESSING CELL QUANTIFY ANY FREE POLYETHYLENE GLYCOL AND POLYETHYLENE GLYCOL-DERIVATIZED MOLECULES
- Patent Title (中): 抗聚乙二醇抗体表达细胞定量任意的聚乙烯甘油和聚乙二醇衍生的分子
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Application No.: US13032317Application Date: 2011-02-22
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Publication No.: US20120015380A1Publication Date: 2012-01-19
- Inventor: Tian-Lu Cheng , Steven R. Roffler , Kuo-Hsiang Chuang , Ssu-Jung Lu
- Applicant: Tian-Lu Cheng , Steven R. Roffler , Kuo-Hsiang Chuang , Ssu-Jung Lu
- Applicant Address: TW Kaohsiung City
- Assignee: KAOHSIUNG MEDICAL UNIVERSITY
- Current Assignee: KAOHSIUNG MEDICAL UNIVERSITY
- Current Assignee Address: TW Kaohsiung City
- Priority: TW099123699 20100719
- Main IPC: G01N33/98
- IPC: G01N33/98 ; C12N5/071
Abstract:
In this invention, anti-PEG antibodies or anti-methoxyl-PEG (anti-CH3O-PEG) antibodies were expressed on cell surface which can collocate with a biotinylated anti-PEG antibody (AGP4-Biotin) or biotinylated PEG (PEG-Biotin) to develop a cell-based sandwich ELISA or a cell-based competition ELISA, respectively. Both of these two methods could sensitively quantify free PEG and PEG-modified macromolecules (proteins, nanoparticles and liposomes) as sensitive as nano-gram level.
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