发明申请
- 专利标题: Alkaline Protease Variants
- 专利标题(中): 碱性蛋白酶变体
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申请号: US13266525申请日: 2010-04-26
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公开(公告)号: US20120058928A1公开(公告)日: 2012-03-08
- 发明人: Masatoshi Tohata , Mitsuyoshi Okuda , Tsuyoshi Sato , Keiji Endo , Katsuhisa Saeki
- 申请人: Masatoshi Tohata , Mitsuyoshi Okuda , Tsuyoshi Sato , Keiji Endo , Katsuhisa Saeki
- 申请人地址: JP Chuo-ku
- 专利权人: Kao Corporation
- 当前专利权人: Kao Corporation
- 当前专利权人地址: JP Chuo-ku
- 优先权: JP2009-110792 20090430; JP2009-110793 20090430; JP2010-093307 20100414; JP2010-093339 20100414
- 国际申请: PCT/JP2010/057838 WO 20100426
- 主分类号: C12N9/50
- IPC分类号: C12N9/50 ; C12N15/63 ; C12P21/02 ; C12N1/19 ; C12N1/15 ; C11D3/386 ; C12N15/57 ; C12N1/21
摘要:
An alkaline protease variant derived from an alkaline protease consisting of an amino acid sequence represented by SEQ ID NO: 2 or consisting of an amino acid sequence having an identity of 90% or more therewith, which variant has mutations wherein one or more amino acid residues at positions selected from (a) position 6, (b) position 15, (c) position 16, (d) position 65, (e) position 66, (f) position 82, (g) position 83, (h) position 204, (i) position 319, and (j) position 337 of the amino acid sequence represented by SEQ ID NO: 2, or at positions corresponding thereto are substituted with the following amino acid residues: (a) or a position corresponding thereto: Typ, Leu, Val, Ile, Met, Tyr, Gln, Lys, Thr, Phe, Arg, Ser, Cys, Ala, or His; (b) or a position corresponding thereto: Glu, Met, Asp, Val, Gln, Arg, Cys, Trp, Ala, or Phe; (c) or a position corresponding thereto: Met, Glu, Arg, Val, Lys, Phe, Tyr, Ile, His, Asp, or Cys; (d) or a position corresponding thereto: Trp; (e) or a position corresponding thereto: His, Trp, Ser, or Leu; (f) or a position corresponding thereto: Ala, Glu, Gln, Ser, Cys, Gly, H is, Lys, Arg, Met, or Asn; (g) or a position corresponding thereto: Ala, Ser, or Cys; (h) or a position corresponding thereto: Glu, Asp, Cys, Val, Thr, Pro, His, Ile, Trp, Ser, Asn, Lys, or Arg; (i) or a position corresponding thereto: Trp, Val, Thr, Leu, Ile, Cys, Glu, Lys, Tyr, Arg, Phe, Gln, Met, Pro, Asp, Asn, H is, or Ser; and (j) or a position corresponding thereto: Arg, Gly, Ser, Lys, Gln, Thr, His, Ala, Cys, or Val.
公开/授权文献
- US08778650B2 Alkaline protease variants 公开/授权日:2014-07-15